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Detecting kit for authenticating cervical cancer susceptibility

A detection kit and cervical cancer technology, applied in the fields of molecular biology and biomedicine, can solve the problems related to the susceptibility of cervical cancer that have not been reported in the literature, and achieve the effects of easy promotion and application, high specificity and low cost

Active Publication Date: 2019-02-19
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have reported that certain single nucleotide polymorphisms (SNPs) in the hOGG1 coding region are associated with the oncogenicity of various tumors (including esophageal cancer, prostate cancer, and lung cancer), but there is no literature report on the SNP positions in the hOGG1 gene Points associated with cervical cancer susceptibility

Method used

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  • Detecting kit for authenticating cervical cancer susceptibility
  • Detecting kit for authenticating cervical cancer susceptibility
  • Detecting kit for authenticating cervical cancer susceptibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Correlation analysis between hOGG1 gene single nucleotide polymorphism detection and cervical cancer susceptibility

[0031] 1.1 Research object

[0032] Randomly selected 400 cases of cervical squamous cell carcinoma and 400 cases of cervical precancerous lesion CIN III, the selected cases were from June 2004 to December 2008, from the Department of Obstetrics and Gynecology, Zhejiang University School of Medicine. For cases with clear diagnosis, 1200 female volunteers with normal physical examination were selected as normal controls. The inclusion criteria of normal controls were no pathological cytology findings, no endometriosis, no gynecological tumors and no other solid cancers or immune disease.

[0033] All cases and normal control individuals were Han residents. All subjects obtained consent in accordance with the requirements of the Ethics Committee of the Gynecological Hospital of Zhejiang University School of Medicine, and all research methods f...

Embodiment 2

[0053] Example 2. hOGG1 gene single nucleotide polymorphism and cervical cancer susceptibility detection kit

[0054] 1. Based on the results of Example 1, we found that the base variation of 7146C>G in SEQ ID NO.1 (rs1052133) was significantly related to the onset of cervical squamous cell carcinoma and its precancerous lesion CIN III, so we designed A kit for detecting peripheral blood DNA templates of patients based on specific primers of hOGG1 gene single nucleotide polymorphisms.

[0055] 2. Prepare a special kit (100tests). The specific reagent composition is shown in Table 4, as follows:

[0056] Table 4. The components of the hOGG1 gene SNP site (rs1052133) detection kit

[0057] Reagent name (concentration)

Total amount of kit

10×PCR Buffer (Mg 2+ 25mM)

250μL

dNTP Mixture (2.5mM each)

1000μL

Taq DNA polymerase (5U / μL)

20 μL

Upstream primer 1 (SEQ ID NO.2) (100pmol)

100μL

Upstream primer 2 (SEQ ID NO.3)...

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Abstract

The invention discloses a detecting kit for authenticating cervical cancer susceptibility, and belongs to the technical field of molecular biology and biological medicine. The detecting kit comprisestwo pairs of specificity primers designed based on the hOGG1 gene mononucleotide polymorphism, the 7146th site marked by a molecule with the nucleotide sequence shown in SEQ ID NO.1 has mononucleotidepolymorphism: C)G, and the hOGG1 gene mononucleotide polymorphism rs1052133(C)G) is closely related to the cervical cancer susceptibility. By means of the PCR technology, the mononucleotide polymorphism in a hOGG1 gene can be effectively detected. The method is simple and rapid in detection, high in specificity, low in cost, free of special DNA sequencing devices and convenient to apply and popularize in various levels of clinical hospitals and has great practical significance in screening cervical cancer high-risk people, detecting susceptible individuals and early preventing, early diagnosing and individually controlling the complicated disease (cervical cancer).

Description

technical field [0001] The invention relates to the technical fields of molecular biology and biomedicine, in particular to a molecular marker for detecting susceptibility to cervical cancer and its application. Background technique [0002] Cervical cancer is the most common gynecological malignancy. The high-incidence age of carcinoma in situ is 30-35 years old, and that of invasive carcinoma is 45-55 years old. In recent years, the onset tends to be younger. With the widespread application of cervical cytology screening, cervical cancer and precancerous lesions can be detected and treated early, so that the incidence and mortality of cervical cancer have been significantly reduced. [0003] High-risk human papillomavirus (HPV) is the main cause of cervical cancer. However, although about 80% of women may be infected with HPV in their lifetime, only a small part of them develop malignant tumors. According to epidemiological surveys and modern molecular genetic studies, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/156
Inventor 陈怀增叶枫王浛知程琪马俊彦陈晓静
Owner ZHEJIANG UNIV
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