Method of converting phlorizin into phloretin
A technology of phloridzin and phloretin, applied in the biological field, can solve the problems of difficult industrialized large-scale production, poor conversion specificity, high cost, etc., achieve good dispersion effect and improve the conversion rate
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Embodiment 1
[0036] Add water into the reaction tank, use hydrochloric acid to adjust the pH value to 3.5, and add the dry powder containing 80% mass fraction of phloridin into the reaction tank according to the ratio of phloridin-containing raw material to water mass ratio of 1:10. Heating to 45°C, adding the mixed enzyme of cellulose compound enzyme and pectin compound enzyme, the mass ratio of mixed enzyme and phloridin raw material is 1:10, and the mass ratio of cellulose compound enzyme and pectin compound enzyme is 1:1 , 45°C for 10 hours for enzymatic hydrolysis; every 0.5 hours, nitrogen gas is continuously introduced from the bottom of the solution for 1 minute to obtain an enzymatic hydrolysis feed solution; the obtained enzymatic hydrolysis feed liquid is heated to 75°C and inactivated for 10 minutes; a cloth bag centrifuge is used Solid-liquid separation is carried out to obtain wet crystals of phloretin, and dry powdered phloretin products are obtained after drying. The conver...
Embodiment 2
[0038] Add a concentration of 0.5% ethanol aqueous solution to the reaction tank, use hydrochloric acid to adjust the pH value to 5.5, and make the dry powder containing 92.5% mass fraction phlorizin according to the ratio of phloridin-containing raw material to ethanol mass ratio of 1:40 Add to reaction tank. Heating to 55°C, adding the mixed enzyme of cellulose compound enzyme and pectin compound enzyme, the mass ratio of mixed enzyme and phloridin raw material is 1:40, and the mass ratio of cellulose compound enzyme and pectin compound enzyme is 1:100 , 55 ℃ heat preservation enzymolysis for 96 hours; every 2 hours from the bottom of the solution for 5 minutes to continuously pass nitrogen gas to obtain the enzymolysis feed solution; heat the obtained enzymolysis feed solution to 100 ℃, inactivate for 45 minutes; use suction filtration irrigation Solid-liquid separation is carried out to obtain wet crystals of phloretin, and dry powdered phloretin products are obtained afte...
Embodiment 3
[0040] Add a concentration of 5% ethanol aqueous solution to the reaction tank, use hydrochloric acid to adjust the pH value to 4.5, and add the dry powder containing 99% mass fraction of phlorizin according to the ratio of phloridin-containing raw material to ethanol mass ratio of 1:25 in the reaction tank. Heat to 52°C, add the mixed enzyme of cellulose compound enzyme and pectin compound enzyme, the mass ratio of mixed enzyme to phloridin raw material is 1:25, the mass ratio of cellulose compound enzyme and pectin compound enzyme is 1:2 , 52 ℃ heat preservation enzymolysis for 20 hours; every 1 hour from the bottom of the solution into the nitrogen for 3 minutes to obtain the enzymolysis feed solution; the obtained enzymolysis feed solution was heated to 80 ℃, inactivated for 30 minutes; plate and frame press The filter is used for solid-liquid separation to obtain wet crystals of phloretin, which are then dried to obtain dry powder phloretin products. The conversion rate ...
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