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Moringa oleifera cordyceps militaris and cultivation method and antibacterial spray thereof

A culturing method and a technology for Cordyceps militaris, applied in the field of Cordyceps militaris and its culturing method and bacteriostatic spray field, can solve the problems such as the content of cordycepin is not greatly improved, the pain of patients is increased, the content of cordycepin is low, etc. High content of cordycepin, good bacteriostatic effect and simple steps

Inactive Publication Date: 2019-02-19
蒋涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing antibacterial or antibacterial drugs often have obvious irritation, which greatly increases the suffering of patients.
[0003] Studies have shown that Cordyceps can not only inhibit the growth of bacterial microorganisms, but also promote the healing of wounds, and the medicinal properties of Cordyceps are mild and non-irritating. The higher the content of cordycepin, the better its antibacterial effect, but wild In Cordyceps, the content of cordycepin is low, which greatly restricts its medicinal value, and the content of cordycepin in the existing artificially cultivated Cordyceps has not been greatly improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of cultivation method of Moringa oleifera Cordyceps militaris, it comprises the steps:

[0028] (1) Preparation of nutrient solution: The nutrient solution is made from the following ratio: 25g of glucose, 1g of triamine citrate, 1g of magnesium sulfate, 3g of potassium dihydrogen phosphate, vitamin B 1 10mg, vitamin B 12 10mg, water 1000ml.

[0029] (2) Prepare the medium: mix the dry base and the nutrient solution at a ratio of 1:1.4 and stir evenly to make a medium, in which the dry base includes 10% Moringa oleifera leaves, 80% silkworm chrysalis and 10% quinoa .

[0030] (3) Inoculation: inoculate the strains into the culture medium prepared in step (2), and perform sterilization treatment for 12 hours.

[0031] (4) Sterilization: Put the sterilized culture medium in step (3) into a sterile darkroom for 15 days of sterilizing treatment, and keep the sterilizing temperature at 18°C.

[0032] (5) Illumination: Transfer the culture medium treated with bacte...

Embodiment 2

[0038] A kind of cultivation method of Moringa oleifera Cordyceps militaris, it comprises the steps:

[0039] (1) Preparation of nutrient solution: The nutrient solution is made of the following ratio: 20g of glucose, 1.5g of triamine citrate, 0.8g of magnesium sulfate, 5g of potassium dihydrogen phosphate, vitamin B 1 5mg, vitamin B 12 5mg, water 1000ml.

[0040] (2) Prepare the medium: mix the dry base and the nutrient solution at a ratio of 1:1.3 and stir evenly to make a medium, in which the dry base includes 10% Moringa leaves, 80% silkworm chrysalis and 10% quinoa .

[0041] (3) Inoculation: inoculate the strains into the culture medium prepared in step (2), and perform sterilization treatment for 12 hours.

[0042] (4) Sterilization: Put the sterilized culture medium in step (3) into a sterile darkroom for 15 days of sterility treatment, and keep the temperature of 19°C.

[0043] (5) Illumination: Transfer the culture medium treated with bacteria in step (4) to a li...

Embodiment 3

[0049] A kind of cultivation method of Moringa oleifera Cordyceps militaris, it comprises the steps:

[0050] (1) Preparation of nutrient solution: The nutrient solution is made from the following ratio: 22g of glucose, 1.2g of triamine citrate, 1.2g of magnesium sulfate, 1g of potassium dihydrogen phosphate, vitamin B 1 15mg, vitamin B 12 15mg, water 1000ml.

[0051](2) Prepare the medium: mix the dry base and the nutrient solution at a ratio of 1:1.5 and stir evenly to make a medium, in which the dry base includes 10% Moringa leaves, 80% silkworm chrysalis and 10% quinoa .

[0052] (3) Inoculation: inoculate the strains into the culture medium prepared in step (2), and perform sterilization treatment for 12 hours.

[0053] (4) Sterilization: Put the sterilized culture medium in step (3) into a sterile darkroom for 15 days of sterility treatment, and keep the temperature of 19°C.

[0054] (5) Illumination: Transfer the culture medium treated with bacteria in step (4) to a...

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Abstract

The invention discloses a moringa oleifera cordyceps militaris cultivation method, moringa oleifera cordyceps militaris cultivated according to the method and antibacterial spray prepared from the moringa oleifera cordyceps militaris. The moringa oleifera cordyceps militaris cultivation method is simple in step, and the cultivated moringa oleifera cordyceps militaris is high in cordycepin content.The antibacterial spray prepared from the moringa oleifera cordyceps militaris has excellent antibacterial effects and is effective in promotion of wound healing, mild in efficacy and free of irritation.

Description

technical field [0001] The invention relates to a Moringa oleifera Cordyceps militaris, a cultivation method thereof and an antibacterial spray. Background technique [0002] In modern clinical treatment, if the wound surface is not treated properly after treatment, it is easy to cause wound infection and affect wound healing. Therefore, it is necessary to clean the wound after treatment. Cleaning is to clean the cuticle and subcutaneous fat necrotic tissue of the skin infection, and then apply some antibacterial or antibacterial drugs. However, the existing bacteriostatic or antibacterial drugs often have obvious irritation, which greatly increases the suffering of patients. [0003] Studies have shown that Cordyceps can not only inhibit the growth of bacterial microorganisms, but also promote the healing of wounds, and the medicinal properties of Cordyceps are mild and non-irritating. The higher the content of cordycepin, the better its antibacterial effect, but wild In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/185A61K9/12A61P17/02A61K35/64C12R1/645
CPCA61P17/02A61K9/0014A61K35/63A61K35/64A61K36/068A61K36/185C12N1/14A61K2300/00
Inventor 蒋涛
Owner 蒋涛
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