A kind of pseudorabies live vaccine diluent and its preparation method and application

Abstract

The invention discloses a diluent of live pseudorabies vaccine, which contains microcrystalline cellulose and astragalus polysaccharide; and also discloses a method for preparing the diluent, which includes the following steps: taking microcrystalline cellulose and adding it to a reaction container filled with water for injection , stir, control the temperature, let stand, then add astragalus polysaccharide, stir and mix, sterilize, and obtain. The advantages of the present invention are: it can improve the effect of nasal drop immunity of pseudorabies vaccine, enhance the place-occupancy effect of pseudorabies virus in the cranial nerve and lymphatic system, intramuscular injection of immunization can produce antibodies in advance, prolong the maintenance time of high-level antibodies, and then improve Pig immunity. Effective prevention of latent infection of pseudorabies wild virus provides a guarantee for the prevention and eradication of pseudorabies. The preparation method of the diluent of the present invention has the characteristics of easy production, low cost, convenient use and the like.

Description

technical field [0001] The invention belongs to the technical field of vaccine processing, in particular to a diluent of pseudorabies live vaccine and its preparation method and application. Background technique [0002] my country is the largest breeding country in the world, with about 1 billion (heads) of pigs, cattle, and sheep slaughtered annually. In recent years, due to the ravages of animal infectious diseases, a large number of livestock and poultry deaths have been caused, which has seriously endangered the healthy development of the breeding industry. After the level of large-scale breeding is improved, the epidemic disease is more likely to spread, and the task of animal epidemic prevention and control is very arduous. However, in recent years, immunosuppressive diseases such as porcine ring disease and blue ear disease have been common in farmers all over the country, which has exacerbated the difficulty of preventing and controlling animal diseases. The phenom...

AI Technical Summary

Problems solved by technology

Intranasal immunization of newborn piglets mainly plays an early occupying effect to prevent the invasion of wild viruses. Although it will not be interfered by maternal antibodies, the residence time of PBS buffer or saline as a diluent in the nasal cavity and respiratory tract is too short, and the occupying place Insufficient amount of vaccine virus also affects the effect of nasal drop immunity

In order to solve the above-mentioned problems such as the small number of vaccine viruses occupying the space and the interference of immunosuppressive diseases, low antibody titer, long production time, and short antibody maintenance time after immunization, this patented technology is proposed

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