A kind of rapid propagation method of test-tube seedling of highland barley

A technology for test-tube seedlings and highland barley, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as optimization of basic medium for genotypes without explants, methods to be optimized, etc., and achieves good application prospects. , the callus grows well, and the effect of increasing yield

Active Publication Date: 2021-05-28
AGRI RES INST TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI +1
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AI Technical Summary

Problems solved by technology

[0005] At present, the research on the rapid propagation of barley in vitro mainly focuses on the optimization of hormone combination, but there are no reports on the optimization of explant genotype (variety), basic medium, etc.; based on this, the best hormone in the prior art Combinations may only be applicable in the case of common species and common basal media, methods provided are subject to optimization

Method used

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  • A kind of rapid propagation method of test-tube seedling of highland barley
  • A kind of rapid propagation method of test-tube seedling of highland barley
  • A kind of rapid propagation method of test-tube seedling of highland barley

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036]EXAMPHI SME test tube seedlings

[0037]1 call injury

[0038]After disinfecting the cyan (ZYM0439) seeds, inoculated in the callus induced medium. The heam-induced medium is based on MS medium, and also contains 1 g / l (NH4) 2SO4, 1.25 mg / l CusO4, 0.5 g / L glutamine, 0.5 g / l - pramine, 0.8 g / L Water-free protein, 30 g / L sucrose, 10 g of mannitol, 1.5 mg / l toxic acetonia, 1.5 mg / l 2, 4-D, 0.2 mg / lkt and 3.5 g / l plant gel.

[0039]After 14 days of lighting, the induction of callus was observed.

[0040]2 burst

[0041]The above induction of good callus was inoculated in the heaming medium, which also contains 1 g / l (NH4) 2SO4, 1.25mg / L CusO4, 0.25mg / L CusO4, 0.25mg / L CusO4, 0.25 mg / L CusO4, 0.25mg / L CusO4, 0.25mg / L CusO4, 0.25 mg / L CusO4, 0.25mg / L CusO4, 0.25 mg / L CusO4, 0.25 mg / L CusO4, 0.25mg / L CusO4, 0.55mg / L CusO4, 0.25 mg / L CusO4, 0.25 mg / L CusO4, 0.5 G / L glutamine, 0.5 g / L pramine acid, 0.8 g / L hydrolyzed, 30g / L sucrose, 10g mannit...

experiment example 1

[0116]Effect of Experimental Example 1 Genotype and Basic Medium on Callus of Qingxi

[0117]This experimental case selected a total of 24 blue-race varieties of 9 series of blue-race varieties were induced, and their vaccination was placed on 44 basic medium, placed under light culture, and statistical callus induced after 14 days.

[0118]The varieties are Gan Qing (No. 3, No. 5), respectively; Kang Qing (No. 6); ZYM (0254, 0439, 0762, 0850, 0957); Dongqing (No. 18, 19); Xira (No. 3 , No. 10, No. 19, 22); Beiqing (No. 1, 7); Kunlun (No. 1, No. 8, No. 14, No. 15); Tibetan (25, 52, 148, 320, 311) , 2000, 7000); C1.

[0119]It is possible to induce a variety of callus, as shown in Table 1 to 8, and the title is the title of the medium.

[0120]Table 1 Qingyu 'Kunlun Series' Callus Induction Results

[0121]

[0122]

[0123]Table 2 Qingyu 'Xrat series' callus induced results

[0124]

[0125]Table 3 Qingyu 'Dongqing series' Callus induced results

[0126]

[0127]Table 4 Qingyu 'ZYM Series' Callus Induction Results

[...

experiment example 2

[0145]Experimental example 2 Effect of culture medium hardness on the heaming success

[0146]The hardness of the plant gel regulation induced medium gel adjusted induced medium, and the effect of the culture medium hardness on the heil of the heave was added to the addition of 0, 4, 6, 8 mg / L. Such asfigure 2 As shown, in the figure, P41MSK2T4P is medium MS + 1 g / l (NH4) 2SO4 + 0.2 g / lknO3 + 1.25mg / l CusO4 + 0.5 g / l glutamine + 0.5 g / L-pramination + 0.8g / L Water-free protein + 30 g / L sucrose + 10 g of mannitol + 4 mg / L toxic acetate + 0.2 mg / lkt + 3.5 g / l plant gel. The P4 represents a viper atrium content of 4 mg / L, 1M represents a mannitol content of 10g, and the SK represents 1 g / l (NH4) 2SO4 and 0.2g / lkno3, 2T representative added 0.2mg / LKT, 4P representing plant gel The content is 4g / L; other identifiers are pushed in this class.

[0147]The results show that with the increase of plant gel content, the medium of culture is also increased, and the colo...

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Abstract

The invention provides a method for rapid propagation of test-tube seedlings of highland barley, which uses highland barley seeds as explants to obtain test-tube plantlets through callus induction, callus subculture and callus differentiation. The present invention also provides the formulation of the culture medium used in the aforementioned method. The invention can effectively realize the rapid propagation of the highland barley, and has a good application prospect in the field of cultivation of the highland barley test-tube seedlings.

Description

Technical field[0001]The present invention relates to the field of plant compositions, and more particularly to a high-proof method of cyanic test tube seedlings.Background technique[0002]Qingyu is a unique gray plant in the west of my country. Qingli planting about 3,500 years of history in the Qinghai-Tibet Plateau, extends from material culture to the field of spiritual culture, has formed a rich cultural culture in the Qinghai-Tibet Plateau, and has a rich and rich national characteristic. Qingyu has important medicinal value and nutritional value, and Qingyu is the main raw material of Tibet's first 糌粑. Because of its geographical location and environmental climate, Qingli has always been the main food crop of Tibetan people.[0003]The breeding goal of the Qingxi is mainly concentrated in production, resistance to biological and non-biological stress. However, the traditional genetic breeding method has certain limitations. The growth of plant gene projects in the end of the 20t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/005
Inventor 曾兴权徐齐君原红军王玉林韦泽秀巴桑玉珍于明寨扎桑尼玛扎西
Owner AGRI RES INST TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
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