Biosensor based on immobilized phellinus igniarius thallus

A technology of biosensor and Phellinus linteus is applied in the field of bioengineering to achieve the effects of real-time detection, improved speed and sensitivity, and simple operation.

Active Publication Date: 2019-02-01
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although there are many Phellinus fungi that secrete laccase, they really have a high yield of laccase, and can directly use the laccase in the fermentation broth or the Phellinus thallus that catalyzes the reaction with laccase without concentration and crude extraction. rare

Method used

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  • Biosensor based on immobilized phellinus igniarius thallus
  • Biosensor based on immobilized phellinus igniarius thallus
  • Biosensor based on immobilized phellinus igniarius thallus

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Preparation of Phellinus Phellinus Membrane 8

[0061] The present invention isolates a new type of Phellinus thallus, which is preserved in the General Microbiology Center of China Microbial Strain Preservation Management Committee. The address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. On August 3, 2018, it was classified and named Inonotus baumii.

[0062] Aseptically transfer the Phellinus thallus on the original slant to fresh PDA medium, and cultivate it at a constant temperature at 28°C until the mycelium is overgrown to activate the Phellinus thallus, and store it at 4°C for future use.

[0063] Put the seed culture medium into the triangular flask, insert the activated Phellinus thallus into the seed culture medium according to the aseptic requirements, cultivate it for 3 days at 28°C and 180r / min, observe the growth of the mycelia, and obtain the Phellinus spp. body seed liquid;

[0064] Wherein the PDA medium comprises potato 200g / L, gl...

Embodiment 2

[0073] Such as figure 2 As shown, the present embodiment provides a biosensor based on immobilized Phellinus thalli including gold electrode 10, porous layer 9, Phellinus bacterium film 8, microporous filter membrane 1, rubber O-ring 7 and elastic electrode card Cover 6; Porous layer 9, Phellinus bacterium membrane 8 and microporous filter membrane 1 are sequentially arranged on the surface of one end of gold electrode 10 from the inside to the outside; One end of the microporous filter membrane 1 is provided, and the outer edge of the microporous filter membrane 1 covering the Phellinus bacterium film 8 is pressed and fixed on the surface of the gold electrode 10 through the end of the elastic electrode ferrule 6. The ring 7 is embedded in the elastic electrode ferrule 6 and arranged outside the microporous membrane 8 to fasten the microporous membrane 1 again.

[0074] Wherein, a kind of preparation method of the biosensor based on immobilized Phellinus thallus is as follo...

Embodiment 3

[0078] A method for detecting chlorophenols in waste water with a biosensor based on immobilized Phellinus thallus, the present embodiment detects through a three-electrode system, wherein the three-electrode system includes an Ag / AgCl electrode as a reference electrode, and a platinum disk The electrode is the counter electrode, and the biosensor is used as the working electrode, and the chlorophenols in the wastewater are determined by cyclic voltammetry; specifically, the following steps are included:

[0079] (1) Qualitatively detect and calibrate 2,4-dichlorophenol and 2,6-dichlorophenol through a three-electrode system to determine the oxidation peak potentials of 2,4-dichlorophenol and 2,6-dichlorophenol and according to the changes of 2,4-dichlorophenol and 2,6-dichlorophenol concentrations and currents, respectively establish their linear regression equations; wherein the three-electrode system includes the Ag / AgCl electrode as the reference electrode, and the platinum...

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Abstract

The invention relates to a phellinus igniarius thallus, which capable of directly growing on the surface of a hydrophilic microporous membrane of a saturated adsorption medium to form a phellinus igniarius film; the phellinus igniarius film forms a biosensor based on immobilized phellinus igniarius thallus; the biosensor includes a gold electrode, a porous layer, the phellinus igniarius film, a microporous membrane, a rubber O-shaped ring, and an elastic electrode sleeve; the porous layer, the phellinus igniarius film and the microporous membrane are arranged on the one end surface of the goldelectrode from the inside to the outside; the elastic electrode sleeve is a hollow cylindrical structure; the elastic electrode sleeve is fixed on the outside of the gold electrode, and the outer edge of the microporous membrane covering the phellinus igniarius film is pressed on the surface of the gold electrode; the rubber O-shaped ring is embedded in the elastic electrode sleeve and disposed outside the microporous membrane, and the microporous membrane is fastened again. The biosensor based on immobilized phellinus igniarius thallus is used for detecting chlorophenol substances in wastewater so as to improve the anti-interference ability of the chlorophenol determination and reduce the detection cost.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a biosensor based on immobilized Phellinus thallus. Background technique [0002] Chlorophenols (CPs) are a class of xenobiotics with high toxicity and serious pollution, which widely exist in industrial production such as pharmaceuticals, printing and dyeing, textiles, petrochemicals, and pesticides. After entering the water body, it is easy to enrich and accumulate in the organism, resulting in strong toxicity. 2,4-dichlorophenol and 2,6-dichlorophenol, as the main chlorophenol compounds, are important pollutants of water pollution, causing great harm to the natural environment, and have been listed as environmental priority by many countries One of the monitored pollutants. Therefore, it is of great practical significance to monitor and control 2,4-dichlorophenol and 2,6-dichlorophenol in wastewater to prevent them from harming humans. [0003] At present, for the determinatio...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H15/00G01N27/48C12R1/645
CPCA01H15/00C12N1/14C12N1/145C12R2001/645G01N27/48
Inventor 王丙莲马耀宏孟庆军杨艳公维丽郑岚杨俊慧刘庆艾
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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