Method for using castanea mollissima flower water extract to improve animal semen quality

A technology of water extract and chestnut flower, applied in the field of improving the quality of animal semen, to achieve the effects of promoting sperm production, expanding the scope of utilization, and improving the quality of semen

Active Publication Date: 2019-02-01
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that there are many active ingredients in chestnut flowers, which have anti-oxidation, anti-aging, anti-inflammatory and other effects. However, it is unknown whether the active ingredients in chestnut flowers also have the effect of improving semen quality, and there is no literature on the use of chestnut flowers. Method of water extract to improve semen quality

Method used

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  • Method for using castanea mollissima flower water extract to improve animal semen quality
  • Method for using castanea mollissima flower water extract to improve animal semen quality
  • Method for using castanea mollissima flower water extract to improve animal semen quality

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: preparation Chinese chestnut flower water extract

[0029] 1) Pick fresh chestnut male flowers in full flowering stage and immediately place them in ultrapure water with a solid-to-liquid ratio of 35g / L. Use a homogenizer to fully homogenize chestnut flowers, and then ultrasonically extract them in an ultrasonic instrument with a power of 780W at 45°C for 1 hour. Subsequently, it was centrifuged at 12000r / min for 50min, the aqueous layer solution was collected, and the obtained aqueous layer solution was freeze-dried to obtain a light yellow powder;

[0030] 2) The light yellow powder obtained in step 1) is dissolved in ultrapure water again, and the ultra-microfiltration membrane with a pore size of 0.25 μm is used for suction filtration, and the aqueous solution obtained by suction filtration is freeze-dried again to obtain a solid powder of chestnut flower water extract ;

[0031] 3) The solid powder of chestnut flower water extract obtained in step 2)...

Embodiment 2

[0032] Embodiment 2: Mouse feeding test

[0033] 240 3-week-old mice (with uniform body weight) of the healthy ICR strain were randomly assigned to 8 groups (30 / group), and the mice were eating and drinking normally, and were raised in a unified SPF rat room . Take a certain amount of chestnut flower water extract powder obtained in step 3) of Example 1 every day according to the design in Table 1, dissolve it in purified water, and prepare the required chestnut flower water extract solution. Each mouse was fed with the water extract solution of chestnut flower matching its body weight by intragastric administration on time every day. Each mouse was gavaged with 0.1 mL water extract solution of chestnut flower. After 5 weeks of gavage, the semen of the mice was collected, and the sperm motility and density were analyzed with a semen quality analyzer. The test results are shown in Table 1:

[0034] Table 1 Effect of water extract of Chinese chestnut flower supplementation o...

Embodiment 3

[0037] Embodiment 3: Pig feeding test

[0038] In the cooperative experimental base of our school, 70 healthy weaned (35-day-old) boars were selected and randomly divided into 7 groups (10 heads / group). All pigs are in a large breeding room, eating and drinking normally, and are under unified management. Take a certain amount of chestnut flower water extract powder obtained in step 3) of embodiment 1 according to the design in Table 2 every day, and fully mix the weighed animal concentrate and chestnut flower water extract powder. Feed the prepared feed concentrate containing chestnut flower water extract to pigs on time every day. Feed until 6 months old, collect the semen of the pigs, and use the semen quality analyzer to analyze the sperm motility and density.

[0039] Table 2 Effects of pig supplementation with chestnut flower water extract on semen quality

[0040] Daily Supplement Dose of Chestnut Flower Water Extract

[0041] Experiments have found that the...

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Abstract

The present invention relates to a method for using a castanea mollissima flower water extract to improve animal semen quality. The castanea mollissima flower water extract is supplemented by a mannerof conducting gavage or adding into feeds every day after weaning of male mammals; an ingesting dosage for the small-sized male mammals is 0.01-20 mg / kg body weight; and an ingestion dosage for the middle-sized male mammals is 0.1-20 mg / kg feeds. The castanea mollissima flower water extract is freeze-dried powder obtained by further ultrafiltration after ultrasonic extraction. The method for using ultrasonic to extract castanea mollissima flower water extract and conducting the further ultrafiltration is provided for the first time; and the castanea mollissima flower water extract supplementsthe feeds for the male mammals and can significantly improve the semen quality of the animals. Supplementary feeding timing starts after the weaning of the male mammals. A technical scheme is to enable the male mammals to better establish a reproductive system, fully exploit genetic potential of the male animals, and expand use scope of the castanea mollissima flower water extract.

Description

technical field [0001] The invention relates to the field of animal reproductive physiology and reproduction technology, in particular to a method for improving the quality of animal semen by using chestnut flower water extract. Background technique [0002] In recent years, due to the excessive or incorrect application of pesticides and fertilizers, environmental pollution, climate change and other reasons, the sperm motility of male animals has decreased, the concentration of sperm has decreased, and the rate of sperm deformity has increased, resulting in a substantial increase in the rate of male infertility. This is a worldwide problem that has attracted widespread attention from all over the world. However, there is no good method to improve semen quality to treat male infertility. Therefore, scientists are looking for effective drugs or methods to improve the semen quality of male animals. [0003] Chestnut (Castanea mollissima BL.), also known as: chestnut, chestnut...

Claims

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Application Information

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IPC IPC(8): A23K10/30
CPCA23K10/30
Inventor 赵勇沈伟孙中义李兰于帅
Owner QINGDAO AGRI UNIV
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