Fungal hypha growth test method in gramineous plant stalks

A technology for the growth of gramineous plants and hyphae, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, and can solve the problems of lack of testing methods

Inactive Publication Date: 2019-01-29
南京胜跃新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the prior art also lacks a test metho...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Simulated botrytis cinerea causes botrytis cinerea on wheat stalks:

[0020] (1) Take a glass tube with a diameter of 20mm, and open a vent hole every 4mm on the side wall of the glass tube, and control the diameter of the vent hole at 0.2mm;

[0021] (2) Seal the bottom of the glass tube with a filter screen, the filter hole diameter of which is 0.25 mm;

[0022] (3) Pour the glass beads with a diameter of 0.4mm into the glass tube;

[0023] (4) Botrytis cinerea is inoculated on the filter screen at the bottom of the glass tube, then the bottom of the glass tube is put into a petri dish, and the culture solution is poured into the petri dish;

[0024] (5) Put the glass tube together with the culture dish into the culture room for 20 days;

[0025] (6) Observing and recording the growth of Botrytis cinerea mycelium in the glass tube to obtain the growth pattern of Botrytis cinerea mycelium in the wheat stalk.

Embodiment 2

[0026] Embodiment 2: Simulating the fungus to cause the fungus on the bentgrass stalks:

[0027] (1) Take a glass tube with a diameter of 10mm, and open a vent hole every 3mm on the side wall of the glass tube, and control the diameter of the vent hole at 0.1mm;

[0028] (2) Seal the bottom of the glass tube with a filter screen, the filter hole diameter of which is 0.2mm;

[0029] (3) Pour the glass beads with a diameter of 0.4mm into the glass tube;

[0030] (4) Inoculate the mycelia of Mycelia syringae on the filter screen at the bottom of the glass tube, then put the bottom of the glass tube into a petri dish, and pour culture solution into the petri dish;

[0031] (5) Put the glass tube together with the culture dish into the culture room for 14 days;

[0032] (6) Observing and recording the growth of Mycelia mycelia in the glass tube to obtain the growth pattern of Mycelia mycelia in the bentgrass stalk.

Embodiment 3

[0033] Embodiment 3: Simulate the syringa fungus and cause the syringa disease in Echinacea:

[0034] (1) Get a glass tube with a diameter of 50mm, and open a vent hole every 5mm on the side wall of the glass tube, and control the diameter of the vent hole at 0.2mm;

[0035] (2) Seal the bottom of the glass tube with a filter screen, the filter hole diameter of which is 0.3 mm;

[0036] (3) Pour the glass beads with a diameter of 0.5 mm into the glass tube;

[0037] (4) Inoculate the mycelium of the fungus Mycelia syringae on the filter screen at the bottom of the glass tube, then put the bottom of the glass tube into a petri dish, and pour the nutrient solution into the petri dish;

[0038] (5) Put the glass tube together with the culture dish into the culture room for 30 days;

[0039] (6) Observing and recording the growth of Mycelia mycelia in the glass tube, and obtaining the growth pattern of Mycelia mycelia in Elymus stalks.

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PUM

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Abstract

The invention discloses a fungal hypha growth test method in gramineous plant stalks. The method comprises the following steps of (1) taking a glass tube with the diameter being 10 to 50mm; forming ventilation holes every 3 to 5mm in the side wall of the glass tube; and controlling the diameter of each ventilation hole to be smaller than 0.2mm; (2) sealing the bottom of the glass tube by a filterscreen, wherein the filter hole diameter of the filter screen is 0.2 to 0.3mm; (3) pouring glass beads with the diameter being 0.4 to 0.5mm into the glass tube; (4) inoculating fungal hyphae onto thefilter screen at the bottom of the glass tube; then putting the bottom of the glass tube into a culture vessel; and pouring a culture solution into the culture vessel; (5) putting the glass tube and the culture vessel into a culture chamber to be cultured for 14 to 30d together; and (6) observing and recording the growth condition of the hyphae in the glass tube to obtain the hypha growth mode. The method is used for studying the fungal hypha growth condition when the gramineous plant stalk fungal disease is caused by fungal pathogenic bacteria.

Description

technical field [0001] The invention relates to a plant stalk simulation test method, in particular to a method for testing fungal mycelium growth in grass plant stalks. Background technique [0002] In scientific research, it is often necessary to simulate the growth of grass plant stems to understand the growth of fungal diseases in grass plant stems. [0003] However, the prior art still lacks a test method for studying the growth of fungal mycelium in grass stems. Contents of the invention [0004] Purpose of the invention: Aiming at the deficiencies and defects of the prior art, the present invention provides a method for studying the growth of fungal hyphae in grass stems when fungal pathogens cause grass fungal diseases. Test Methods. [0005] Technical solution: a method for testing the growth of fungal hyphae in grass stems of the present invention is characterized in that it includes the following steps: [0006] (1) Take a glass tube with a diameter of 10 mm ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCC12Q1/02
Inventor 孙嘉斌孙晓哲魏秀慧
Owner 南京胜跃新材料科技有限公司
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