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Microbial quorum sensing signal molecular quenching strain and application thereof in biological control of diseases

A quorum sensing signal, microorganism technology, applied in microorganism-based methods, microorganisms, microorganisms, etc., to achieve good degradation activity, good adaptation, and significant effects

Active Publication Date: 2019-01-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the deficiencies of existing microbial quorum sensing signal molecule quenching bacteria, and provide a new high-efficiency microbial quorum sensing signal molecule DSF / AHLs quenching bacteria, namely Acinetobacter ( Acinetobacter seifertii ) F15Y, this strain has a significant degradation effect on various quorum sensing signal molecules including DSF, OHHL and OdDHL, and also has the characteristics of fast growth, simple cultivation method, strong adaptability, and not easy to mutate, which has great promotion Application Potential

Method used

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  • Microbial quorum sensing signal molecular quenching strain and application thereof in biological control of diseases
  • Microbial quorum sensing signal molecular quenching strain and application thereof in biological control of diseases
  • Microbial quorum sensing signal molecular quenching strain and application thereof in biological control of diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Isolation and identification of Acinetobacter strain F15Y

[0050] 1. Isolation and screening of strain F15Y

[0051] (1) Soil sample collection: The soil collected from sweet potato fields cultivated for many years was used as the microbial source.

[0052] The soil samples were collected on March 16, 2017 from the sweet potato field soil cultivated all year round by Nanhai District and Shunlu Gangchang, Foshan City, Guangdong Province. The soil from the surface to the depth of 5 cm was sampled, bagged, and preserved as a microbial source for strain isolation .

[0053] (2) Enrichment culture of strains: Prepare MSM medium, put 50 mL of MSM medium into a 250 mL Erlenmeyer flask for sterilization, add DSF mother solution under aseptic conditions after cooling (the concentration of the mother solution is 100mM, methanol is the solvent ), so that the final mass concentration of DSF was 0.01 mM. At the same time, 5 g of soil samples were added, cultured on a s...

Embodiment 2

[0069] Example 2 Antibiotic susceptibility analysis of bacterial strain F15Y

[0070] In order to better study the biocontrol potential of the strain F15Y obtained in Example 1, we studied the antibiotic sensitivity of the strain F15Y. As shown in Figure 4, the resistance of the strain F15Y to carbenicillin (CARB) reached 400 μg·mL -1 or above, the resistance to ampicillin (AMP) reaches 300 μg·mL -1 or above, the resistance to gentamicin (GEN), chloramphenicol (CM), kanamycin (KAN), streptomycin (STR), neomycin sulfate (NEO) reaches 50 μg·mL -1 .

[0071] This result is helpful for the selection of appropriate antibiotics as a reference for the prevention and treatment of plant diseases in subsequent studies.

Embodiment 3

[0072] Example 3 Determination of the growth and degradation DSF relationship curve of strain F15Y

[0073] 1. Pick a single colony of strain F15Y and inoculate it in LB medium to pre-culture to the logarithmic phase. After the obtained bacterial solution is centrifuged at 4000rpm for 5 minutes, the supernatant is discarded, and the bacterial cells are washed with 0.9% sterile normal saline and resuspended. , as a seed suspension, and then inoculated into 50 mL MSM basal medium at an inoculation volume of 1:100, and added DSF mother solution to make the final concentration 2 mM, cultivated at 30°C, 200 rpm for 24 h, and took samples regularly. Collect samples at different time points and measure OD by spectrophotometer 600 The value indicates the growth of strain F15Y, and the residual amount of DSF determined by HPLC indicates the degradation of DSF by strain F15Y.

[0074] 2. The HPLC test results are shown in Figure 5 (Figure A is the control picture of the uninoculated s...

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Abstract

The invention discloses a microorganism quorum sensing signal molecule quenching strain and an application thereof in biological control of diseases. The quenching strain is acinetobacter F15Y, whichwas deposited in Guangdong Microbial Culture Collection Center on August 28, 2018 with the deposit number of GDMCC No:60440. The strain has high degradation activity of DSF and AHLs, high degradationefficiency, remarkable effect and stable degradation performance. It has a good control effect on pathogens and related plant diseases which depend on DSF or AHLs. The strain F15Y was isolated from the soil of sweet potato field which was cultivated all the year round. It could adapt to the environment and was friendly to the environment. Therefore, the strain F15Y has great popularization and application potential in the prevention and control of plant pathogenic fungi which rely on DSF or AHLs to cause diseases, and the invention can reduce the problems of antibiotic abuse and pesticide residue pollution, and provides a new idea for biological control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of disease biological control. More specifically, it relates to a microbial quorum sensing signal molecule quenching bacterium and its application in biological disease control. Background technique [0002] my country is a big country in the production and use of pesticides and fertilizers. Non-standard use of pesticides has led to prominent ecological and environmental problems, causing many plant pathogens to develop resistance. Therefore, people increase the amount of pesticides used to prevent and control plant diseases. Such a vicious circle will cause environmental pollution and crops. Pesticide residues pose a potentially huge threat to human health. [0003] Biological control is a new control method advocated in recent years, and quorum quenching based on quorum sensing is a hot spot in biological control. Quorum sensing (QS) refers to signaling molecules that regulate the expression of specific g...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P1/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 陈少华何杰华林子秋叶田罗青青李昊张炼辉
Owner SOUTH CHINA AGRI UNIV
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