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Stevioside extraction method and application

An extraction method and technology of steviol glycosides, which are applied in the field of extraction of active ingredients of plants and steviosides in stevia leaves, can solve the problems of supplementing nutrients and improving oral health, oral health risks, and safety risks at the same time, and achieve Effective in eliminating acne, improving intestinal tract, and promoting micro-ecological balance

Inactive Publication Date: 2019-01-25
广州联淘生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the jelly prepared by this method has unique flavor, moderate elasticity and toughness, and has health care and beauty effects, but the rich raw materials and sugar contained in the jelly are easy to remain after eating, and there is a certain hidden danger to oral health.
[0009] Chinese patent publication CN108210385A discloses a jelly mouthwash with anti-caries, antibacterial and root-fixing effects. The mouthwash contains moisturizing ingredients, solvents, acid-base buffers, anti-caries ingredients, root-strengthening ingredients, flavoring ingredients, and antiseptic ingredients. etc., and the anti-caries composition is a chemically synthesized substance with a complex structure. Although the mouthwash has the effects of anti-caries, antibacterial and root-fixing teeth, the chemically synthesized composition contained in the mouthwash has certain potential safety hazards. , and the mouthwash needs to be used alone, and cannot achieve the dual effects of supplementing nutrients and improving oral health at the same time

Method used

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  • Stevioside extraction method and application
  • Stevioside extraction method and application
  • Stevioside extraction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 01

[0057] Put 50 kg of stevia leaf and dilute ethanol solution of extractant into the extraction tank for extraction. The volume percentage of dilute ethanol solution is 20%. The first extraction uses 50 kg of extractant for 5 hours, and the second extraction operation uses 50 kg of extracting agent, continued for 2 hours, and mixed the extracts of the two extractions as steviol glycoside extracts;

[0058] Add flocculant ferrous sulfate solution and calcium oxide to the extract, flocculate for 1 hour, wherein the mass ratio of the ferrous sulfate solid mass in the ferrous solution to the extract is 0.6:100, the added calcium oxide and ferrous sulfate solid The mass ratio is 0.1:50, and the flocculated extract is filtered to obtain the steviol glycoside filtrate;

[0059] Use an adsorption column to absorb the inulin filtrate, then wash the column with 3mol / L sodium hydroxide solution to pH 8.5, pure water to pH 7.0, and then 1mol / L sulfuric acid solution to pH 6.0. Wash the col...

Embodiment 02

[0063] The difference from Example 01 is that the extractant used in the multi-stage extraction process is a dilute ethanol solution with a volume percentage of 40%. After the flocculant is added in the flocculation link, the flocculation is carried out for 3 hours, and the total glycosides in the finally obtained stevioside powder product The content is 95%.

[0064] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0065] Basic embodiment

[0066] An enzyme jelly, comprising the following components by weight: 8-25 parts of fruit and vegetable enzyme powder, 5-10 parts of fructooligosaccharide, 5 parts of white sugar, 1 part of passion fruit puree, 1-5 parts polydextrose, 4 p...

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Abstract

The invention relates to a stevioside extraction method which sequentially includes the steps: multistage extraction; flocculation filtering; desalination and discoloration; concentration; sterilization; drying. The multistage extraction is characterized in that stevia leaf raw materials are extracted fro 2-3 times by the aid of an extraction agent, prepared extracts are collected and combined andused for next step, the extraction agent is provided with thin ethyl alcohol solution with the volume percent of 10-40%, a flocculating agent used in the flocculating process is provided with ferroussulfate solution and calcium oxide, adsorption of an adsorption column is used in the desalination and discoloration process, and washing and desorption are implemented by the aid of alkali, acid, deionized water and 70% of alcoholic solution after adsorption. By the multistage extraction method, the utilization rate of raw materials is increased, an extraction process is relatively simple, production cost is reduced, impurity removal operations such as desalination and discoloration of extracted crude products are implemented in the preparation process, the purity of a final product is effectively ensured, and subsequent production and use of the finally prepared product can be directly implemented.

Description

technical field [0001] The invention relates to an extraction method, in particular to an extraction method of plant active ingredients, more specifically, to an extraction method of steviol glycosides in stevia leaves. Background technique [0002] Stevia, also known as Stevia sugar, is an extract of Stevia rebaudiana leaves. It does not contain sugar and calories, and has no obvious toxic and side effects. It can inhibit high blood sugar and high blood pressure, and has anti-inflammatory and antidiarrheal properties. Diuretic, assist immune regulation and so on. Steviol glycoside (Stevia) is specifically a kind of sweet terpene glycoside, with white to yellowish color, pleasant taste and no peculiar smell. It is a new sugar source with broad development prospects. At present, it is widely used in industries such as cosmetics, food and medicine. [0003] In the article "Research on the Extraction and Purification Process of Stevioside", the extraction process of steviosid...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H15/256A23L21/10A23L33/125
CPCC07H1/08A23L21/10A23L33/125A23V2002/00C07H15/256A23V2200/3202A23V2200/30A23V2250/28A23V2250/5042A23V2250/032A23V2250/5036A23V2250/262A23V2250/642
Inventor 吴力卡
Owner 广州联淘生物科技有限公司
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