Nano fluorescent probe and its preparation method and application

A nano-fluorescent probe and nano-gold technology, applied in the field of nano-fluorescent probes and their preparation, can solve the problems of increased detection cost, waste of resources and the like

Active Publication Date: 2021-03-23
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this type of detection method is usually one-off, that is, it can only be used for one detection, resulting in waste of resources and increased detection costs.

Method used

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  • Nano fluorescent probe and its preparation method and application
  • Nano fluorescent probe and its preparation method and application
  • Nano fluorescent probe and its preparation method and application

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preparation example Construction

[0036] The embodiment of the present invention also provides a method for preparing the above-mentioned fluorescent probe, comprising the following steps:

[0037] Preparation of matrix fibers: In the presence of one-dimensional organic templates, surface sulfhydryl-modified SiO was prepared through molecular recognition and catalytic induction of organic templates 2 Nanofibers as matrix fibers;

[0038] Synthesis of nano-gold solution: use glutathione as a reducing agent to reduce chloroauric acid, and synthesize glutathione-protected nano-gold particle solution;

[0039] Modification of gold nanoparticles: disperse the matrix fiber in water, mix with the gold nanoparticle solution, and fully stir the reaction, so that the gold nanoparticles are anchored on the SiO through coupling with thiol groups. 2 surface, after centrifugation, washing and drying, one-dimensional SiO 2 / Au nanocomposites;

[0040] Modified Hg 2+ ions: in the 1D SiO 2 Add mercury ion solution to the ...

Embodiment 1

[0057] A preparation method for a one-dimensional nanometer fluorescent probe, comprising the following steps:

[0058] Preparation of matrix fibers: dissolving amphiphilic short peptide molecules in water at a concentration of 16, ultrasonically dispersing, adjusting the pH value to 7, and standing at room temperature to obtain a one-dimensional amphiphilic short peptide molecular self-assembly aqueous solution. The short peptide is x m Y n type short peptide, X is isoleucine, Y is lysine, m=3, n=1; add 2 ml of ethanol and 0.3 mmol of orthosilicon to the aqueous solution of one-dimensional amphiphilic short peptide molecular self-assembly Acetate methyl ester, adjust the pH value to 8, vortex to disperse evenly, let it stand for 2 days for mineralization reaction, and complete the first mineralization; add 0.03 mmol of mercaptopropyl tri Ethoxysilane, after mixing evenly, let it stand for 2 days for mineralization reaction to complete the second mineralization; after comple...

Embodiment 2

[0068] A preparation method for a one-dimensional nanometer fluorescent probe, comprising the following steps:

[0069] (1) Preparation of matrix fibers: dissolving amphiphilic short peptide molecules in water at a concentration of 8 mM, ultrasonically dispersing, adjusting the pH value to 6, and standing at room temperature to obtain a one-dimensional amphiphilic short peptide molecular self-assembly aqueous solution. Short peptide is X m Y n type short peptide, X is isoleucine, Y is lysine, m=3, n=1; add 1mL of methanol and 0.4mmol of orthosilicic acid to the aqueous solution of one-dimensional amphiphilic short peptide molecular self-assembly Methyl ester, adjust the pH value to 9, vortex to disperse evenly, let it stand for 1 day for mineralization reaction, and complete the first mineralization; add 0.02 mmol of mercaptotriethoxy to the material obtained from the first mineralization Silane, after mixing evenly, let it stand for 3 days for mineralization reaction to com...

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Abstract

The invention provides a nano-fluorescent probe as well as a preparation method and application thereof. The nano-fluorescent probe is a composite nanomaterial, a surface sulfydryl modified SiO2 nanofiber is taken as a matrix fiber, gold nanoparticles and Hg<2+> ions are modified on the surface of the matrix fiber, the gold nanoparticles are connected to the matrix fiber through sulfydryl, and Hg<2+> ions are attached to the nanomaterial through metal bonds formed by the Hg<2+> ions and Au<+>; and the particle size of the gold nanoparticles is less than 3 nm, the radial size of the nano-fluorescent probe is in nanoscale, and the axial size of the nano-fluorescent probe is in micron-scale. The probe can be used for qualitative and quantitative detection of glutathione and is simple to operate, high in sensitivity, recyclable and higher in economic value, and the preparation method has the advantages of being simple, energy-saving and environment-friendly.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probe sensing in analytical chemistry, and in particular relates to a nano fluorescent probe and its preparation method and application. Background technique [0002] Glutathione (GSH) exists in all normal biological cells and can protect the sulfhydryl groups of proteins in organisms to maintain various biological functions of proteins. At the same time, GSH participates in many physiological processes including biomass metabolism, intracellular signal transduction and gene regulation. In particular, the consumption of GSH is closely related to diseases such as diabetes, cardiovascular disease, and nerve tissue degeneration. GSH is a biomarker molecule, and the accurate measurement of trace GSH can provide assistance for the diagnosis and treatment of related diseases. [0003] At present, the detection methods of glutathione mainly include liquid chromatography, capillary electrophoresis, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 王生杰修阳崔丙顺夏永清曹美文
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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