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A direct-throwing microbial agent and its application in wet corn soaking

A technology of microbial agents and microbial strains, applied in the field of microbial agents, can solve the problems of difficult transportation, low stability, and short storage period of lactic acid bacteria liquid, so as to improve efficiency, increase yield, and enhance freeze-drying survival rate Effect

Active Publication Date: 2020-04-28
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the lactic acid bacteria liquid has limitations such as short storage period, low stability and difficult transportation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The screening of embodiment 1LAB 0#, LAB 1#

[0034] Take 10mL of corn soaking water (old pulp) and dilute to 10% of the original concentration respectively.-1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 and 10 -7 times, and then take 0.3mL respectively spread on the MC plate, (50 ± 2) ℃ culture 24h. Pick a single colony with a calcium-dissolving circle, streak it on a plate, separate it, and purify it repeatedly. The colonies with large and unique calcium-dissolving circles were isolated and stored on the slant, and they were numbered LAB0# and LAB 1# respectively. The corn soaking water (old pulp) is provided by the corn starch production workshop of Linghua Group Co., Ltd. (Jining, Shandong).

[0035] MC medium formula: soybean peptone 5.0g / L, beef extract powder 3.0g / L, yeast extract powder 3.0g / L, glucose 20.0g / L, lactose 20.0g / L, calcium carbonate 10.0g / L, agar 15.0g / L, neutral red 0.05g / L, pH value at 25°C is 6.0±0.1.

Embodiment 2

[0036] The preparation of embodiment 2 microbial inoculum A

[0037] (1) Preparation of seed liquid: insert thermophilic lactic acid bacteria LAB 0# into MRS liquid medium, and cultivate seed liquid for 24 hours at 50-55°C on a shaker at 100r / min; the formula of MRS liquid medium is One liter of MRS liquid medium contains peptone 10.0g, beef extract 5.0g, yeast extract 4.0g, glucose 20.0g, Tween-80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, triammonium citrate 2.0g , magnesium sulfate 0.2g, manganese sulfate 0.05g, and the balance is distilled water;

[0038] (2) Preparation of bacterium slime: the seed liquid that step (1) obtains is inserted in the fermentation medium by the inoculum size of 5%, after 100r / min shaker culture at 50-55 ℃ for 24 hours, the fermented product is centrifuged ( Centrifugal speed is 5000r / min, centrifugation time 10min), discard the supernatant, collect the thallus, wash with normal saline to obtain the bacterium sludge; where...

Embodiment 3

[0042] The preparation of embodiment 3 microbial inoculum A

[0043] (1) Preparation of seed liquid: insert thermophilic lactic acid bacteria LAB 0# into MRS liquid medium, and cultivate seed liquid for 24 hours at 50-55°C on a shaker at 120r / min; the formula of MRS liquid medium is One liter of MRS liquid medium contains peptone 10.0g, beef extract 5.0g, yeast extract 4.0g, glucose 20.0g, Tween-80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, triammonium citrate 2.0g , magnesium sulfate 0.2g, manganese sulfate 0.05g, and the balance is distilled water;

[0044] (2) Preparation of bacterium slime: the seed liquid that step (1) obtains is inserted in the fermentation medium by the inoculum size of 5%, after 120r / min shaker culture at 50-55 ℃ for 24 hours, the fermented product is centrifuged ( Centrifugal speed is 5000r / min, centrifugation time 15min), discard supernatant, collect thalli, wash with normal saline, promptly obtain bacterium slime; Wherein the ...

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Abstract

The invention relates to a direct-throwing type microbial bacterial agent and its application in wet corn soaking. The direct-throwing microbial bacterial agent is composed of thermophilic lactic acid bacteria LAB 1# and a freeze-drying protective agent; the thermophilic lactic acid bacteria LAB Culture preservation information of 1#: name of preservation unit: General Microbiology Center of China Committee for Microbial Culture Collection; address of preservation unit: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; preservation date: 2018 April 18, 2009; deposit number: CGMCC No.15624; classification name: Staphylococcus succinus.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, and in particular relates to a direct-throwing microbial microbial agent and its application in wet corn soaking. Background technique [0002] The traditional process uses old pulp and new acid (about 0.2% sulfurous acid solution) to soak together in the soaking tank. The complete soaking process includes adding old pulp to the new tank, adding new acid to corn that has been soaked for a long time, and tank to tank There is a regular cycle in between. This circulation process can maintain a certain concentration difference between the corn and the soluble substances in the soaking liquid, and soak the soluble substances in the corn. However, the sulfur dioxide produced in the traditional immersion process is the main component of acid rain, which will cause environmental pollution. At the same time, sulfurous acid will corrode equipment and pipelines and increase production costs. In ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C08B30/04C08B30/02C12R1/225
CPCC08B30/02C08B30/044C12N1/04C12N1/20C12N1/205C12R2001/225
Inventor 刘庆艾史建国马耀宏马恒公维丽蔡雷郑岚杨俊慧
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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