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Rapid multiplication method for tissue culture of golden camellia

A technology of tissue culture and camellia japonica, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem of inability to quickly and effectively expand the population of camellia japonica, limit the development of Camellia japonica tissue culture seedlings, and slow proliferation of clustered buds, etc. problem, achieve the effect of shortening the disinfection time, avoiding bacterial contamination and promoting proliferation

Inactive Publication Date: 2019-01-18
吕诗林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Camellia can be propagated by traditional sowing, cutting and grafting methods, but none of the above methods can quickly and effectively expand the population of Camellia
In order to save this endangered plant, many researchers have tried to use tissue culture to obtain tissue culture seedlings, and have achieved certain results. However, in the existing tissue culture process of Camellia japonica, there are still slow proliferation of clustered buds and a multiplication coefficient. The low problem limits the development of Camellia japonica tissue culture seedlings

Method used

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  • Rapid multiplication method for tissue culture of golden camellia
  • Rapid multiplication method for tissue culture of golden camellia

Examples

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Embodiment 1

[0020] A tissue culture rapid propagation method for Camellia sinensis, comprising: step 1, cutting off the young stem tips of Camellia sinensis, and after disinfection treatment, to obtain explants; step 2, inducing and culturing the explants through clustered buds , get the clump bud; Step 3, the described clump bud is cultivated through rooting and strong seedlings, obtains Camellia sinensis seedlings;

[0021] Wherein, the clump bud induction culture is specifically as follows: transferring the explants into the clump bud induction medium, culturing under dark conditions for 2 d, and then culturing under the conditions of a light intensity of 1500 lux and a light time of 14 h / d 15d, then add a 1mm high volume concentration of 70% alcohol solution to the cluster bud induction medium, cultivate for 2h under dark conditions, and then add a 2mm high conditioning solution to the cluster bud induction medium, at Cultivated under dark conditions for 2d, and finally cultivated for...

Embodiment 2

[0027] A tissue culture rapid propagation method for Camellia sinensis, comprising: step 1, cutting the young stem tips of Camellia sinensis, and after disinfection treatment, to obtain explants; step 2, inducing and culturing the explants through cluster buds , get the clump bud; Step 3, the described clump bud is cultivated through rooting and strong seedlings, obtain golden flower tea seedlings;

[0028]Wherein, the clump bud induction culture is specifically as follows: transferring the explants into the clump bud induction medium, culturing under dark conditions for 2 d, and then culturing under the conditions of a light intensity of 1600 lux and a light time of 14 h / d 15d, then add a 1mm high volume concentration of 70% alcohol solution to the cluster bud induction medium, cultivate for 2h under dark conditions, and then add a 2mm high conditioning solution to the cluster bud induction medium, at Cultivated under dark conditions for 2 d, and finally cultivated for 15 d u...

Embodiment 3

[0034] A tissue culture rapid propagation method for Camellia sinensis, comprising: step 1, cutting off the young stem tips of Camellia sinensis, and after disinfection treatment, to obtain explants; step 2, inducing and culturing the explants through clustered buds , get the clump bud; Step 3, the described clump bud is cultivated through rooting and strong seedlings, obtains Camellia sinensis seedlings;

[0035] Wherein, the clump bud induction culture is specifically as follows: transferring the explants into the clump bud induction medium, culturing under dark conditions for 2 d, and then culturing under the conditions of a light intensity of 1600 lux and a light time of 14 h / d 15d, then add a 1mm high volume concentration of 70% alcohol solution to the cluster bud induction medium, cultivate for 2h under dark conditions, and then add a 2mm high conditioning solution to the cluster bud induction medium, at Cultivated under dark conditions for 2d, and finally cultivated for...

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Abstract

The invention discloses a rapid multiplication method for the tissue culture of golden camellia, which comprises induction culture of clustered shoots, specifically, in the midway of the induction culture of clustered shoots, under a dark condition, 1mm of alcoholic solution with the volume concentration of 70 percent and 2mm of regulator are sequentially added into clustered shoot induction culture medium, wherein the clustered shoot induction culture medium is 3 / 4 MS plus 3.0-4.0mg / L 6-KT plus 1.5-2.5mg / L ZT plus 1.0-2.0mg / L IBA plus 1.0-2.0mg / L NAA, and the regulator is 3.0-4.0mg / L ZT plus10.0-13.0mg / L GA3. According to the invention, the multiplication coefficient of golden camellia is up to 4.8 to 5.0 times, the abnormal seedling rate is as low as 0.6 percent, the seedling survival rate is up to 94.1 percent, and the average seedling height is 92mm.

Description

technical field [0001] The present invention relates to the field of tissue culture. More specifically, the present invention relates to a tissue culture rapid propagation method of Camellia sinensis. Background technique [0002] Camellia sinensis belongs to the Camellia family and the genus Camellia, and is as famous as the precious "living plant fossils" such as silver fir, cypress, dove tree, etc. It is a plant species in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, and it is called the magical Oriental in foreign countries. Magic tea, known as "plant world giant panda", "tea queen". Camellia oleifera can be propagated by traditional sowing, cutting and grafting methods, but none of the above methods can rapidly and effectively expand the population of Camellia oleifera. In order to save this endangered plant, many researchers have tried to obtain tissue culture seedlings by tissue culture, and have achieved certai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/00A01H4/008
Inventor 吕诗林
Owner 吕诗林
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