Hydrogen peroxide biosensor based on sulfonated carbon nanotube composited with horseradish peroxidase, preparation and application thereof
A composite technology of horseradish peroxidase and nanotubes, which is applied in the direction of instruments, scientific instruments, and electrochemical variables of materials, can solve the problems of unfavorable non-pollution detection of the system to be tested, unfavorable hydrogen peroxide sensors, etc., and achieve good application Foreground, improvement of hydrophilicity, effect of good electron transport
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Embodiment 1
[0044] A method for preparing a hydrogen peroxide biosensor based on sulfonated carbon nanotube composite horseradish peroxidase, comprising the following steps:
[0045] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;
[0046] (2) Place 100 mg of acidified carbon nanotubes (acidified carbon nanotubes) in 100 mL of distilled water for ultrasonic dispersion for 30 minutes to obtain a suspension of 1 mg / mL acidified carbon nanotubes; dissolve 72 mg of sodium nitrite in 40 mL of distilled water, Cool to 0-5°C to obtain an aqueous solution of sodium nitrite; then add 184mg of p-aminobenzenesulfonic acid and 2g of hydrochloric aci...
Embodiment 2
[0052] A method for preparing a hydrogen peroxide biosensor based on sulfonated carbon nanotube composite horseradish peroxidase, comprising the following steps:
[0053] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;
[0054] (2) Put 100 mg of acidified carbon nanotubes into 100 mL of distilled water for 30 minutes to obtain a suspension of 1 mg / mL acidified carbon nanotubes; dissolve 72 mg of sodium nitrite in 40 mL of distilled water and cool to 0-5°C , then 184mg p-aminobenzenesulfonic acid and 2g hydrochloric acid aqueous solution (1mol / L) were added into the sodium nitrite aqueous solution and mixed evenly, and ice-bat...
Embodiment 3
[0063] A method for preparing a hydrogen peroxide biosensor based on sulfonated carbon nanotube composite horseradish peroxidase, comprising the following steps:
[0064] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder is polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, taken out, rinsed with distilled water, and dried at room temperature to obtain a pretreated glassy carbon electrode;
[0065] (2) Put 100 mg of acidified carbon nanotubes into 100 mL of distilled water for 30 minutes to obtain a suspension of 1 mg / mL acidified carbon nanotubes; dissolve 72 mg of sodium nitrite in 40 mL of distilled water and cool to 0-5°C , followed by adding 184mg p-aminobenzenesulfonic acid and 2g hydrochloric acid aqueous solution (1mol / L) into the sodium nitrite aqueous solution and uniformly mixing, ice ...
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