Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A gene mutation site for eliminating low-fertility Hu sheep and application thereof

A technology of mutation site and fecundity, applied in the field of molecular biology

Active Publication Date: 2019-01-15
NANJING AGRICULTURAL UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although scientists have discovered a number of major genes (such as BMP15, BMPR1B, GDF9, Woodlands and B4GALNT2 genes) that control sheep reproductive traits (mainly including lamb size and ovulation number) in foreign sheep breeds, a total of 15 genes have been identified. SNPs loci, but except the FecB locus of BMPR-IB gene exists in Hu sheep, other loci are not related to the high fecundity traits of Hu sheep

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A gene mutation site for eliminating low-fertility Hu sheep and application thereof
  • A gene mutation site for eliminating low-fertility Hu sheep and application thereof
  • A gene mutation site for eliminating low-fertility Hu sheep and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Experimental materials

[0023] Choose healthy Hu sheep ewes with no disease and good body condition, and record the number of lambs born. Ear tissue samples were collected with ear size forceps and stored at -20°C for extraction of genomic DNA.

[0024] 2. Genomic DNA extraction

[0025] Genomic DNA was extracted from ear tissues of Hu sheep by conventional phenol / chloroform extraction method and stored at -20°C.

[0026] 3. Primer design

[0027] According to the sheep genome sequence (GenBank ID: NC_019463.2), use Primer5.0 software to design a pair of primers, the forward primer sequence is: 5'-TGT GTA GGA AGT TGA ACC CCT-3' (SEQ ID NO.1), The reverse primer sequence is: 5'-GAA TAA GGT TGA GAG GGA TGT GT-3' (SEQ ID NO.2). Primers were synthesized by Nanjing Qingke Biotechnology Co., Ltd.

[0028] 4. PCR amplification

[0029] The PCR amplification system is 10 μL, mainly including: 0.5 μL Hu sheep genomic DNA, 0.5 μM forward primer, 0.5 μM reverse primer, 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a gene mutation site for eliminating low-fertility Hu sheep and application thereof, wherein the mutation site is located at 1354 site of the 3'-UTR of the Hu sheep BMPR1B gene, the mutation is G to A, the average litter size per litter of Hu ewes with GA genotype is 0.54 lower than that of Hu ewes with GG genotype, and the transcriptional activity of 3'-UTR of the BMPR1B gene is only half of that of G-type. Animal reproductive trait is a low heritability trait, genetic progress is slow with conventional breeding techniques, As that mutation site lead to obvious reduction of reproductive performance of the breeding Hu sheep ewe, the low-fecundity genotype individuals can be eliminated and the high-fecundity genotype individuals can be retain by adopting a simple genotyping technique to detect the mutation site, and the mutation site has important economic value for speeding up the breeding progress of the high-fecundity Hu sheep population or strain.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a gene mutation site for eliminating low fecundity Hu sheep and its application. Background technique [0002] Hu sheep is a famous sheep breed in my country. It is one of the first 138 national livestock and poultry genetic resource protection breeds by the Ministry of Agriculture, and one of the 42 local sheep breeds or resources included in the Chinese sheep breed catalog. Hu sheep are mainly distributed in the Jiangsu, Zhejiang and Shanghai regions in the Taihu Lake Basin. The central production areas are located in Suzhou City in Jiangsu Province, Huzhou City and Jiaxing City in Zhejiang Province. Hu sheep is also the only sheep breed in southern my country. It has excellent germplasm characteristics such as high-quality lamb skin, estrus in four seasons, early sexual maturity, high multiple births, resistance to humidity and heat, fast early growth, tender and delicious meat, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李齐发杜星姚一龙曹秋瑜
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com