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Method for separation and purification of D-lactic acid from D-ammonium lactate fermentation broth

A technology for separation, purification and fermentation liquid, applied in the field of biological engineering, can solve the problems of low product quality, long process route, many unit operations, etc., and achieve the effects of high product quality, low operating energy consumption, and no waste liquid pollution.

Inactive Publication Date: 2019-01-15
SINOPEC YANGZI PETROCHEM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defects of long process route, many unit operations, serious pollution of waste liquid, high energy consumption, low recovery rate and low product quality in the existing technology of lactic acid extraction, the invention provides a kind of fermentation liquid derived from D-ammonium lactate Method for separating and purifying D-lactic acid in

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of D-ammonium lactate fermented liquid is as follows:

[0040] (1) Plate culture: Bacillus BS1-5 was inoculated into the plate medium for anaerobic culture, the culture temperature was 30°C, and the culture time was 48 hours;

[0041] (2) Seed culture: Inoculate the bacillus cultured on the plate in step (1) into the seed medium for anaerobic culture, the culture temperature is 30° C., and the culture time is 24 hours;

[0042] (3) Fermentation acid production: inoculate the seed culture solution obtained in step (2) into the fermentation medium to carry out fermentation acid production, the inoculum size is 15%, the fermentation temperature is 30 ° C, and nitrogen is fed to maintain its anaerobic environment. The neutralizer controls the pH of the fermentation system at 6.0.

[0043] The composition of the above-mentioned plate medium is (g / L): 10 glucose, 1 yeast extract, 1 anhydrous sodium acetate, 0.1 anhydrous magnesium sulfate, 1 potassium dihydro...

Embodiment 2

[0048] (1) Plate culture: Inoculate Bacillus BS1-5 into plate medium for anaerobic culture, culture temperature is 45°C, culture time is 20h;

[0049] (2) Seed culture: Inoculate the bacillus cultured on the plate in step (1) into the seed medium for anaerobic culture, the culture temperature is 45° C., and the culture time is 12 hours;

[0050] (3) Fermentation acid production: inoculate the seed culture liquid obtained in step (2) into the fermentation medium to carry out fermentation acid production, the inoculum size is 3%, the fermentation temperature is 45°C, feed nitrogen to maintain its anaerobic environment, adopt The neutralizer controls the pH of the fermentation system at 7.0.

[0051] The composition of the above-mentioned plate medium is (g / L): 30 glucose, 3 yeast extract, 4 anhydrous sodium acetate, 0.4 anhydrous magnesium sulfate, 3 potassium dihydrogen phosphate, and 25 agar.

[0052] The above-mentioned seed medium composition is (g / L): glucose 40, yeast ext...

Embodiment 3

[0056] (1) Plate culture: inoculate Bacillus BS1-5 into plate medium for anaerobic culture, culture temperature is 37°C, culture time is 24h;

[0057] (2) Seed culture: Inoculate the bacillus cultured on the plate in step (1) into the seed medium for anaerobic culture, the culture temperature is 37°C, and the culture time is 20h;

[0058] (3) Acid production by fermentation: Inoculate the seed culture solution obtained in step (2) into the fermentation medium to produce acid by fermentation, the inoculum size is 10%, the fermentation temperature is 37° C., and nitrogen is fed to maintain its anaerobic environment. The neutralizer controls the pH of the fermentation system at 6.5.

[0059] The composition of the above-mentioned plate medium is (g / L): 20 glucose, 2 yeast extract, 2 anhydrous sodium acetate, 0.3 anhydrous magnesium sulfate, 2 potassium dihydrogen phosphate, and 20 agar.

[0060] The composition of the above seed medium is (g / L): 20 glucose, 2 yeast extract, 2 pe...

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Abstract

The invention discloses a method for separation and purification of D-lactic acid from D-ammonium lactate fermentation broth. The method includes the following steps in order: 1) conducting solid-liquid separation and removal of protein and pigment on D-ammonium lactate fermentation broth to obtain clarified fermentation broth; 2) concentrating the clarified fermentation broth obtained in step 1);3) conducting acidification and crystallization operations on the concentrated fermentation broth obtained in step 2); 4) filtering the liquid-solid mixture obtained in step 3) to obtain the by-product ammonium sulfate; 5) subjecting the crystalized fermentation broth containing D-lactic acid to ion exchange; 6) carrying out nanofiltration decolorization on the fermentation broth subjected to ionexchange; and 7) concentrating the fermentation broth subjected to nanofiltration so as to obtain D-lactic acid. The D-lactic acid extraction method has the characteristics of simplicity, easy operation, no waste liquid pollution, low energy consumption, high recovery and high product quality.

Description

technical field [0001] The invention relates to a method for separating and purifying D-lactic acid from D-ammonium lactate fermentation liquid, belonging to the technical field of bioengineering. Background technique [0002] Lactic acid is one of the three major organic acids in the world, it can be widely used in food, medicine, chemical industry and agriculture and other fields. The special D-lactic acid can be widely used in the fields of medicine, chemical industry and agriculture. Polylactic acid synthesized with D-lactic acid as a monomer has become the most promising green environmental protection material in the 21st century due to its good biodegradability and other excellent use characteristics (such as transparency, thermoplasticity, product safety, etc.). In addition, herbicides such as "Puma" and "Weiba", which are made of high-purity D-lactic acid, are new types of low-toxicity pesticides, which can effectively kill weeds and insects and are economical and r...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C59/08C12P7/56C12R1/07
CPCC07C51/42C12P7/56C07C59/08Y02P20/59
Inventor 方晓江刘经伟李泽壮杨爱武
Owner SINOPEC YANGZI PETROCHEM
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