PCR laboratory with environmental barrier
A laboratory and barrier technology, applied in the field of PCR laboratory, can solve problems such as incorrect direction of gas flow pressure gradient, false positive test results, contaminated reagents and samples, etc., to achieve true test results, control positive false positives, and prevent gas mutual penetration leaky effect
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Embodiment 1
[0059] Such as Figure 1-3 The shown PCR laboratory with environmental barriers includes a reagent preparation room 1, a sample preparation room 2, an amplification room 3 and a product analysis room 4 arranged sequentially from left to right, wherein:
[0060] A first transfer window 12 is provided on the wall between the reagent preparation chamber 1 and the sample preparation chamber 2, and a second transfer window 23 is provided on the wall between the sample preparation chamber 2 and the amplification chamber 3. A third transfer window 34 is provided on the wall between the amplification chamber 3 and the product analysis chamber 4;
[0061]A first buffer chamber 5 is provided on the front side of the reagent preparation chamber 1, a second buffer chamber 6 is provided on the front side of the sample preparation chamber 2, and a third buffer chamber 6 is provided on the front side of the amplification chamber 3. buffer room 7;
[0062] On the front side of the first buf...
Embodiment 2
[0078] A PCR laboratory with an environmental barrier, the rest are the same as in Example 1, the air pressure P1 in the reagent preparation chamber 1=the air pressure P5+5Pa in the first buffer chamber 5;
[0079] Air pressure P5+5Pa in the first buffer chamber 5=air pressure P8+5Pa in the dedicated corridor 8 in the positive pressure zone;
[0080] Air pressure P8 in the special corridor 8 of the positive pressure area = outdoor normal temperature pressure P 0 +5Pa;
[0081] Air pressure P9 in the dedicated corridor 9 in the negative pressure area = outdoor normal temperature pressure P 0 -5Pa;
[0082] Air pressure P7 in the third buffer chamber 7 = air pressure P9-5Pa in the dedicated corridor 9 in the negative pressure zone;
[0083] Air pressure P3 in the amplification chamber 3 = air pressure P7-5Pa in the third buffer chamber 7 .
Embodiment 3
[0085] A kind of PCR laboratory with environmental barrier, all the other are the same as embodiment 2, and described reagent preparation room entrance and exit door 15 is offered toward the direction of reagent preparation room 1;
[0086] The entrance and exit door 26 of the sample preparation chamber is opened toward the direction of the sample preparation chamber 2;
[0087] The entrance and exit door 37 of the amplification chamber is opened towards the direction of the third buffer chamber 7;
[0088] The entrance and exit door 49 of the product analysis room is opened in the direction of the dedicated corridor 9 in the negative pressure area;
[0089] The first buffer door 58 is opened towards the direction of the first buffer chamber 5;
[0090] The second buffer door 68 is opened towards the direction of the second buffer chamber 6;
[0091] The third buffer door 79 is opened in the direction of the dedicated corridor 9 in the negative pressure area;
[0092] The p...
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