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Method for preparing tryptophan, fermentation medium used for the same, and special nutrient element for tryptophan fermentation

A technology of fermentation medium and tryptophan, which is applied in the field of fermentation engineering and enzyme engineering biology, can solve the problems of affecting the metabolic process of tryptophan-producing bacteria, affecting the extraction process of tryptophan, and low utilization rate, so as to improve the color Improve the industrial level of amino acid fermentation, improve the level of fermentation technology, and increase the effect of production capacity

Pending Publication Date: 2019-01-11
江苏澳创生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, the substances used as organic nitrogen sources for tryptophan fermentation include yeast extract, corn steep liquor, wort juice, bean cake hydrolyzate, etc. These organic nitrogen sources are slowly utilized in the process of tryptophan fermentation and cultivation, and the utilization rate is not high, and a lot of them are produced. Impurities, affect the metabolic process of tryptophan-producing bacteria, and incompletely utilized residues affect the post-extraction process of tryptophan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: A method for preparing tryptophan and the fermentation medium and nutrients used for tryptophan fermentation

[0023] Add 500mL of purified water into the beaker, accurately weigh 20g of defatted silkworm chrysalis protein, 20g of yeast powder, and 10g of peptone, put them into the beaker, stir evenly, heat indirectly to 90°C for 15 minutes, and quickly cool down to 55°C. Add 1.6g of compound protease, 1.2g of alkaline protease, and 0.8g of flavor protease, and keep the temperature at 55±1°C for enzymolysis. After 6 hours of enzymatic hydrolysis, heat to 90°C for 10 minutes to inactivate the enzyme. The reaction solution was passed through a microfiltration filter for solid-liquid separation. The microfiltrate was filtered through a 2000 Da laboratory ultrafiltration membrane. Concentrate the ultrafiltrate with a rotary evaporator, and stop concentrating after concentrating to one third of its volume. The concentrated solution was dried by spray drying sma...

Embodiment 2

[0025] Example 2: A preparation method of tryptophan and the fermentation medium used therein and special nutrients for tryptophan fermentation

[0026] Add 200L of purified water to the 2000L enzymatic hydrolysis tank, accurately weigh 8kg of defatted silkworm chrysalis protein, 5kg of yeast powder, and 2kg of peptone into the enzymatic hydrolysis tank, stir evenly, heat indirectly to 90°C for 15 minutes, and quickly cool down to 55°C. Compound protease 0.6kg, alkaline protease 0.6kg, flavor protease 0.3g, keep the temperature at 55±1℃ for enzymolysis. After 8 hours of enzymatic hydrolysis, heat to 100°C for 10 minutes to inactivate the enzyme. The reaction solution was passed through a microfiltration filter for solid-liquid separation. The microfiltrate was filtered through 1000 Da ultrafiltration. Concentrate the ultrafiltrate with vacuum evaporation and concentration equipment, and stop concentrating after concentrating to one-third of the volume. Use a spray dryer to ...

Embodiment 3

[0029] Example 3: A preparation method of tryptophan and the fermentation medium used therein and special nutrients for tryptophan fermentation

[0030] A kind of nutrient element specially used for tryptophan fermentation. Mix defatted silkworm chrysalis protein, yeast powder and peptone to obtain a mixture, then add purified water to the mixture to prepare a feed liquid with a mass fraction of 5%, stir evenly and then heat to 80°C, then cooled to 40°C, and then added 1% protease by weight of the mixture for enzymolysis for 2 hours, then heated the enzymolysis solution to 90°C to inactivate the enzyme, and filtered the enzymolysis solution to obtain the filtrate. The filtrate is concentrated and dried to obtain the nutrient element specially used for tryptophan fermentation.

[0031] A preferred embodiment is: the mixture contains the following materials in mass percentages: 40% defatted silkworm chrysalis protein, 40% yeast powder, and 20% peptone.

[0032] A preferred embo...

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Abstract

The invention provides a method for preparing tryptophan, a fermentation medium used for the same, and a nutrient special for tryptophan fermentation. The method for preparing tryptophan includes thesteps: obtaining a mixture by mixing the defatted silkworm pupa protein, yeast powder and peptone, adding 5 to 20% of purified water into the mixture to prepare a feed liquid, stirring uniformly, heating the mixture to 80 to 100 DEG C, then cooling to 40-60 DEG C, adding 1-10% protease of the mixture to carry out enzymatic hydrolysis for 2-15h, heating the enzymatic hydrolysate to 90-120 DEG C tokill the enzyme, filtering the enzyme hydrolysate to obtain filtrate, concentrating and drying the filtrate to obtain tryptophan fermentation special nutrient element, wherein the tryptophan fermentation special nutrient element is obtained. As the special nutrient element for tryptophan fermentation is use in tryptophan fermentation, the acid production and conversion rate of tryptophan can be greatly improved, and the technical level of tryptophan fermentation can be improve.

Description

technical field [0001] The invention relates to a preparation method of tryptophan, a fermentation medium used therein and a nutrient element specially used for tryptophan fermentation, and belongs to the fields of fermentation engineering and enzyme engineering biotechnology. Background technique [0002] Tryptophan is one of the eight essential amino acids in human and animal life activities, and exists in organisms in a free or combined state. It plays a very important role in the growth, development and metabolism of humans and animals. It is called the second essential amino acid and is widely used in many aspects such as medicine, food and feed. The production of tryptophan mainly relied on chemical synthesis and protein hydrolysis at the beginning, but with the continuous deepening of research on the production of tryptophan by microbial fermentation, this method has become dominant. As the market demand for tryptophan continues to increase, the technology of produci...

Claims

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Application Information

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IPC IPC(8): C12P13/22
CPCC12P13/22
Inventor 周旭波王健方培新王华萱林青平曾建华刘帅
Owner 江苏澳创生物科技有限公司
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