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Extraction method of biotin in seabuckthorn and biotin obtained by the method

An extraction method and biotin technology, applied in the field of biotin, can solve the problems of difficult removal of organic solvents, unfavorable human health, and low safety, and achieve the natural and pure source of biotin, non-toxic extraction method, and high safety Effect

Inactive Publication Date: 2020-03-06
QINGHAI TSINGHUA BIOTRY BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction rate of the above enzymatic extraction method is low, and the organic solvent-assisted extraction method has low safety, and the organic solvent is not easy to completely remove, and there is a risk of adverse human health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for extracting biotin in seabuckthorn, the extraction method comprising: firstly using cellulase whose weight is 0.5% of seabuckthorn to enzymatically hydrolyze seabuckthorn, and at the same time ultrasonically break the seabuckthorn wall, and use an ultrasonic wall breaker for ultrasonic breaking , and then the samples after enzymatic hydrolysis and ultrasonic wall breaking were sequentially filtered, centrifuged and adsorbed to obtain biotin; wherein, the total time of enzymatic hydrolysis and ultrasonic wall breaking was 85min, the centrifugation speed was 2000r / min, and the centrifugation time was 30min. The adsorption adopts AL-2 macroporous adsorption resin.

[0040]The extraction rate of biotin in sea buckthorn by this method is 0.14ppm.

Embodiment 2

[0042] A method for extracting biotin in seabuckthorn, the extraction method comprising: firstly using cellulase whose weight is 3.5% of the weight of seabuckthorn to enzymatically hydrolyze seabuckthorn, and at the same time ultrasonically break the seabuckthorn wall, and use an ultrasonic wall breaker for ultrasonic breaking , and then the samples after enzymatic hydrolysis and ultrasonic wall breaking were sequentially filtered, centrifuged and adsorbed to obtain biotin; wherein, the total time of enzymatic hydrolysis and ultrasonic wall breaking was 66min, the centrifugation speed was 4000r / min, and the centrifugation time was 10min. The adsorption adopts FL-1 macroporous adsorption resin.

[0043] The extraction rate of biotin in seabuckthorn by this method is 0.56ppm.

Embodiment 3

[0045] A method for extracting biotin in seabuckthorn, the extraction method comprising: firstly using cellulase whose weight is 1.5% of seabuckthorn to enzymatically hydrolyze seabuckthorn, and at the same time ultrasonically break the seabuckthorn wall, and use an ultrasonic wall breaker for ultrasonic breaking , and then the samples after enzymatic hydrolysis and ultrasonic wall breaking were sequentially filtered, centrifuged and adsorbed to obtain biotin; wherein, the total time of enzymatic hydrolysis and ultrasonic wall breaking was 100min, the centrifugation speed was 3000r / min, and the centrifugation time was 20min. The adsorption adopts AB-8 macroporous adsorption resin.

[0046] The extraction rate of biotin in seabuckthorn by this method is 0.16ppm.

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Abstract

The invention relates to the field of biotin extraction and in particular provides a method for extracting biotins in sea-buckthorn and biotins obtained by adopting the method. The method for extracting biotins in sea-buckthorn comprises the following steps: performing enzymolysis on the sea-buckthorn with cellulase, performing ultrasonic wall breaking on the sea-buckthorn, and sequentially filtering, centrifuging and adsorbing the samples subjected to enzymolysis and ultrasonic wall breaking, thereby obtaining the biotins. According to the method disclosed by the invention, the sea-buckthornis subjected to enzymolysis with cellulase and then subjected to auxiliary wall breaking by adopting ultrasonic waves, the method disclosed by the invention has high extraction rate compared with a single enzyme extraction method, and has high safety compared with an organic solvent co-extraction method, and impurities in the samples are removed by filtration, centrifugation and adsorption so as to obtain the biotins with high purity.

Description

technical field [0001] The invention relates to the field of biotin extraction, in particular to a method for extracting biotin from seabuckthorn and biotin obtained by the method. Background technique [0002] Seabuckthorn is a deciduous shrub, which is characterized by drought tolerance, wind and sand resistance, and sand-fixing effect. Seabuckthorn is widely planted in Northwest China for desert greening, bringing good economic and sociological benefits to local social and economic development. value. Biotin, also known as vitamin H and coenzyme R, is a water-soluble vitamin and also belongs to the vitamin B family, B7; it is a necessary substance for the synthesis of vitamin C, an indispensable substance for the normal metabolism of fat and protein, and is a kind of maintenance of human body. Nutrients necessary for healthy growth, development and normal body function. [0003] Seabuckthorn contains considerable amounts of vitamins, including targeted components such a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D495/04
CPCC07D495/04
Inventor 龚晨于雪李婷
Owner QINGHAI TSINGHUA BIOTRY BIO TECH
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