A kind of solid enzyme type time-temperature indicator and preparation method thereof

An indicator, enzyme-type technology, applied in the field of solid-state enzyme-type time-temperature indicator and its preparation, can solve the problems of easy leakage of liquid TTI, reduce color indicator, difficult to control, etc., achieve low cost, prolong color change, Avoid uncontrollable effects

Active Publication Date: 2021-06-22
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main disadvantages of enzymes are that they are expensive and relatively unstable, even when used in aqueous media
Therefore, Gray devoted himself to the research of solid-state enzyme-based time-temperature indicator, in the title "Development and Application of a Tyrosinase-Based Time-Temperature Indicator (TTI) for Determining the Quality of Turbot Sashimi" ("Journal of Ocean University of China (English Edition)") )", 2017,16(5):847-854) conducted a preliminary study on the time-temperature indicator system based on tyrosinase, based on the principle of the reaction of tyrosinase and tyrosine , to realize the monitoring of food, which not only reduces the addition of color indicators, but also avoids the shortcomings of liquid TTI that is easy to leak and difficult to control, and can directly adhere to the surface of food or packaging materials, but the indicator time is only 60h, while The storage time of most fresh, fruit and vegetable foods at 4°C is 5-6 days, which is not applicable to the instructions of most foods

Method used

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  • A kind of solid enzyme type time-temperature indicator and preparation method thereof
  • A kind of solid enzyme type time-temperature indicator and preparation method thereof
  • A kind of solid enzyme type time-temperature indicator and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1 Preparation of immobilized tyrosinase dispersion

[0043] (1) Prepare the immobilized tyrosinase dispersion by the following steps:

[0044] (a) Dissolve 2 mg of tyrosinase with an enzyme activity of 915 U / mg in 1 mL of buffer solution with pH = 6.8 to prepare tyrosinase solution, wherein the buffer solution is PBS buffer solution, prepared from Prepared from sodium hydrogen phosphate and disodium hydrogen phosphate;

[0045] (b) preparing sodium alginate solution;

[0046] (c) preparing sodium carboxymethylcellulose solution;

[0047] (d) Mix 1 mL of tyrosinase solution, 5 mL of sodium alginate solution, and 2 mL of sodium carboxymethylcellulose solution, and dilute to 10 mL with water;

[0048] (e) Preparation of calcium chloride (CaCl 2 ) solution, and 100 mL was taken, and the mixed solution prepared in step (d) was dropped into it to obtain immobilized tyrosinase beads;

[0049] (f) Wash the immobilized tyrosinase beads three times with water, then a...

Embodiment 2

[0066] Example 2 Solid enzyme time-temperature indicator and preparation method thereof

[0067] Solid enzyme time-temperature indicator (TTI), including hydrogel film Ⅰ and hydrogel film Ⅱ.

[0068] The hydrogel film I is a circular film with a diameter of 6 cm, and the mixture of 10 g of polyvinyl alcohol aqueous solution and 1 mL of tyrosine aqueous solution is injected into a mold with a diameter of 6 cm to prepare a hydrogel film by freezing-thawing method;

[0069]Hydrogel film II is a circular film with a diameter of 1 cm, which is prepared by freezing and thawing a mixture of 3 g of polyvinyl alcohol aqueous solution and 0.1 to 0.6 g of immobilized tyrosinase dispersion into a mold with a diameter of 1 cm. adhesive film;

[0070] Hydrogel film Ⅰ and hydrogel film Ⅱ are concentrically stacked into a two-layer structure, and then the two-layer structure formed by hydrogel film Ⅰ and Ⅱ is encapsulated in a transparent plastic film, and hydrogel film Ⅰ is not covered with...

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Abstract

The invention belongs to the field of intelligent packaging, and discloses a solid enzyme type time-temperature indicator composed of a polyvinyl alcohol hydrogel film I containing tyrosine and a polyvinyl alcohol hydrogel film containing immobilized tyrosinase Ⅱ is formed by stacking in a closed container. The solid enzyme type time-temperature indicator obtained by the invention has a long indicating time and is easy to store. The invention also discloses a preparation method thereof: comprising the following steps: (1) mixing polyvinyl alcohol aqueous solution and tyrosine aqueous solution, and freezing-thawing method to obtain hydrogel film I; (2) mixing polyvinyl alcohol aqueous solution and The immobilized tyrosinase dispersion was mixed, and the hydrogel film II was obtained by the freezing-thawing method; (3) The hydrogel film I and the hydrogel film II were stacked into two layers, and then sealed in an airtight container, A solid-state enzymatic time-temperature indicator is obtained. The preparation method of the invention has simple process, is easy to operate, and the whole process is in solid state, avoiding the disadvantage that the liquid is not easy to store.

Description

technical field [0001] The invention belongs to the field of intelligent packaging, and in particular relates to a solid enzyme time-temperature indicator and a preparation method thereof. Background technique [0002] Food, especially fresh food, during storage, transportation or sales, temperature fluctuations have a great impact on its quality. In order to avoid food deterioration due to temperature fluctuations, it is necessary to monitor the entire life course of the food; time-temperature indication TTI (TTI) reflects the whole or part of the temperature change history of the indicated product through the cumulative effect of time and temperature to indicate the temperature change history and remaining shelf life of the food, thereby providing consumers with effective food quality change information, consumers Whether food has spoiled can be judged from visual information, which helps to ensure the quality and safety of food. [0003] According to the working principl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 陈金周袁园杨会歌刘旭影牛明军
Owner ZHENGZHOU UNIV
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