Method for culturing bifidobacterium in common incubator, special culture medium and corresponding count method

Abstract

The invention provides a method for culturing bifidobacterium in a common incubator, a special culture medium and a corresponding count method. The special culture medium comprises an MRS-lactose solid medium and a pectin solid medium, wherein the MRS-lactose solid medium is laid on a panel bottom layer and mixed with a bacterial solution, the pectin solid medium is laid on the MRS-lactose solid medium, the panel is then inverted, and placed in the thermostatic incubator with the temperature of 37 DEG C for bacterial culturing. The special culture medium is laid by two layers, which makes theculturing of bifidobacterium in the common thermostatic incubator possible; the method for culturing bifidobacterium in a common incubator can be implemented through the common thermostatic incubator,without anaerobic incubators, anaerobic culture tanks, hungate roll-tubes and the like, and thus, investment input for equipment and medicament is low, operation is simple, and even general laboratory technicians can complete the operation after simple training.

Description

technical field [0001] The invention relates to a method for cultivating bacteria, a special culture medium and a corresponding counting method, in particular to a method for cultivating bifidobacteria in an ordinary incubator, a special culture medium and a corresponding counting method, belonging to the technical field of microorganisms . Background technique [0002] Bifidobacterium (bifidobacterium) is an important physiological bacteria in the intestinal tract of humans and animals, and its existence directly affects the health of the digestive tract. Bifidobacteria, as the main native bacteria, directly participate in the host's digestion, nutrition, metabolism, absorption, immunity and anti-infection processes. [0003] With the continuous deepening of the research on the biological characteristics and microecological significance of bifidobacteria, a large number of microecological preparations of bifidobacteria have emerged. The probiotic preparations prepared usin...

AI Technical Summary

Problems solved by technology

[0006] The use of anaerobic culture technology to cultivate bifidobacteria not only requires a lot of investment in equipment and chemicals, but also is cumbersome and requires operators to have high operational skills.

Due to the different equipment and personnel skills, it is difficult to guarantee the stability of the training batch

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