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Preparation method of sensor for measuring microbial biochemical oxygen demand

A technology of microbial biochemistry and oxygen demand, applied in the direction of material electrochemical variables, instruments, measuring devices, etc., can solve the problems of time-consuming and labor-consuming, cumbersome operation, poor reproducibility, etc., achieve enhanced current signal, low cost, and easy operation Effect

Active Publication Date: 2020-11-06
ZHEJIANG SCI-TECH UNIV
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  • Description
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Problems solved by technology

This method has the advantages of wide application range and low equipment requirements, but the method is time-consuming, cumbersome to operate and poor in reproducibility

Method used

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  • Preparation method of sensor for measuring microbial biochemical oxygen demand

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Embodiment Construction

[0016] The following are specific examples of the present invention to further describe the technical solutions of the present invention, but the present invention is not limited to these examples.

[0017] A method for preparing a sensor for the determination of microbial biochemical oxygen demand, comprising the following steps:

[0018] 1) Polish the glassy carbon electrode on the suede with 1 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and then ultrasonically wash it with ultrapure water and absolute ethanol for 5 minutes to make the surface of the glassy carbon electrode smooth. mirror surface, and then soaked in absolute ethanol to obtain a pretreated glassy carbon electrode;

[0019] 2) Using deionized water as the dispersion medium, prepare a Ketjen Black dispersion with a concentration of 0.5-2.5 mg / ml, and use a pipette gun to take 5 μl of the above Ketjen Black dispersion and apply it to the glassy carbon electrode obtained in step 1). , dried at ...

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Abstract

The invention provides a preparation method of a sensor used for measuring microorganism biochemical oxygen demand (BOD). The preparation method comprises following steps: the surface of a working electrode is loaded with Ketjen Black through drop coating method, after drying, the working electrode modified with Ketjen Black is loaded with a bacterial cellulose / yeast / menadione mixed solution through drop coating method so as to form a carrier coating layer. The carrier coating layer is capable of fixing yeast onto the working electrode, and preventing shedding of Ketjen Black in test process,and the film with response capacity on organic matters in water body is formed. The microorganism fixed on the surface of the electrode is adopted for oxidation degradation of the organic matters, andmeasuring is realized through electrochemical testing method. According to the preparation method, Ketjen Black is taken as a conductive agent; bacterial cellulose is adopted for coating of yeast andpreventing of Ketjen Black in test process, so that test stability is increased. The microorganism biochemical oxygen demand sensor is simple and convenient in operation, low in cost, and rapid and accurate in testing.

Description

technical field [0001] The invention belongs to the technical field of water body detection, and relates to a method for measuring microorganisms, in particular to a method for preparing a sensor for measuring the biochemical oxygen demand of microorganisms. Background technique [0002] With the rapid development of organic chemical industry and other industries, the content of organic compounds in water body has been increasing, which has caused great harm to the environment and human health. However, it is difficult to quantitatively detect each organic component in water, so biochemical oxygen demand (BOD) is commonly used in modern detection technology to measure the degree of organic pollution in water. At present, the five-day biochemical oxygen demand standard dilution method (BOD5) is commonly used at home and abroad to measure, which is defined as the amount of free oxygen consumed by aerobic microorganisms to oxidize and decompose organic matter in a unit volume o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/333G01N27/26
CPCG01N27/26G01N27/30G01N27/3335
Inventor 蔡玉荣赵陈煜姚菊明陈瑶蔡泽玮
Owner ZHEJIANG SCI-TECH UNIV
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