Culture method for improving differentiation from peripheral blood multipotent cells to cartilage cells

Abstract

The invention relates to a culture method for improving differentiation from peripheral blood multipotent cells to cartilage cells, and belongs to the technical field of cytobiology and regenerative medicine. The culture method comprises the following steps: carrying out primary culture on peripheral blood multipotent mesenchymal matrix cells to obtain third-generation cells; co-culturing the third-generation passed-down peripheral blood multipotent mesenchymal matrix cells and human articular chondrocytes in an induction culture medium. According to the culture method for improving the differentiation from the peripheral blood multipotent cells to the cartilage cells, the peripheral blood multipotent stem cells and the chondrocytes are co-cultured and the two types of cells mutually promote proliferation and the differentiation; the number of times of chondrocyte proliferation and passage and the quantity of the chondrocytes can be reduced, and the differentiation is improved; an efficient method is provided for establishing a seed cell source, and a new medical experiment evidence is also provided for enlarging a cell source of cartilage tissue engineering.

Description

technical field [0001] The invention relates to a culture method for improving the differentiation of peripheral blood pluripotent cells into chondrocytes, belonging to the fields of cell biology technology and regenerative medicine. Background technique [0002] Cartilage tissue is a tissue rich in proteoglycans, collagen, and hyaluronic acid. It is composed of chondrocytes and cartilage matrix. It mainly supports and reduces friction in the body. However, cartilage tissue lacks blood vessels, so it is difficult to repair cartilage tissue once it is damaged, even if it is a small cartilage tissue injury, it is difficult to repair naturally. At present, the clinical methods for repairing cartilage tissue damage mainly include cartilage and subchondral bone removal, microfracture and subchondral bone drilling, autologous osteochondral transplantation, allogeneic osteochondral transplantation, cartilage or periosteum transplantation, etc. These methods can repair cartilage ti...

AI Technical Summary

Problems solved by technology

These methods can repair cartilage tissue damage to a certain extent, but they all have obvious disadvantages. For example, microfracture and subchondral bone drilling methods can make stem cells reach the damaged site, but due to different local environments and mechanical stresses, it is difficult for stem cells to recover. Differentiate into chondrocytes; autologous osteochondral transplantation is safe and effective, but it will bring new trauma to patients; allogeneic osteochondral transplantation avoids new trauma caused by autologous osteochondral transplantation, but there are donor source restrictions, immune Risk of rejection and disease transmission, etc.

Current studies have found that the use of peripheral blood pluripotent cells to induce and culture chondrocytes is a very promising technology, but research and development have found that the isolation and culture conditions of peripheral blood pluripotent cells are very complicated, so the formation efficiency of peripheral blood pluripotent cells is low.