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Method of promoting phytoremediation of soil nickel pollution by utilizing pseudomonas vesicularis

A Pseudomonas vesicularis and phytoremediation technology, applied in the biological field, can solve problems such as poor economic benefits, achieve the effect of reducing stress and increasing economic benefits

Inactive Publication Date: 2019-01-01
HUNAN XISI ECOLOGY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a method of utilizing Pseudomonas vesicularis to promote phytoremediation of nickel pollution in soil, so as to overcome the problem of poor economic benefit of existing enrichment plants

Method used

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  • Method of promoting phytoremediation of soil nickel pollution by utilizing pseudomonas vesicularis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Put 30-50ml of liquid medium into a shaker flask for activation; take the activated Pseudomonas vesicularis and put it into the liquid medium for fermentation to make a bacterial liquid, put it into a spectrophotometer, and measure the OD600 concentration to be 0.8 -1.5 fermentation is complete. Each liter of liquid medium includes: 15-18g of gelatin peptone, 10-13g of tryptone, 10-13g of potassium sulfate, 1-2g of magnesium chloride, 15-20g of agar, 100-120ml of glycerol, and water.

[0050] Prepare the bacterial liquid at 0.5mL-2mL / m 2 After spraying in the soil to be repaired, turn over the ground to mix the bacterial solution with the surface soil. The ridges are ridged with a distance of 1.2 meters, a ridge width of 1 meter, and a ridge height of 0.3 meters.

[0051] Open a planting groove with a depth of 5-10cm and a width of 10-15cm on the raised field ridge, and spray 0.5mL-2mL / m in the planting groove 2 Spraying the bacterium solution; soaking the roots of t...

Embodiment 2

[0055] Put 30-50ml of liquid medium into a shaker flask for activation; take the activated Pseudomonas vesicularis and put it into the liquid medium for fermentation to make a bacterial liquid, put it into a spectrophotometer, and measure the OD600 concentration to be 0.8 -1.5 fermentation is complete. Each liter of liquid medium includes: 15-18g of gelatin peptone, 10-13g of tryptone, 10-13g of potassium sulfate, 1-2g of magnesium chloride, 15-20g of agar, 100-120ml of glycerin, and sterile water.

[0056] Prepare the bacterial liquid at 0.5mL-2mL / m 2 After spraying in the soil to be repaired, turn over the ground to mix the bacterial solution with the surface soil. The ridges are ridged with a distance of 1.2 meters, a ridge width of 1 meter, and a ridge height of 0.3 meters.

[0057] Open a planting groove with a depth of 5-10cm and a width of 10-15cm on the raised field ridge, and spray 0.5mL-2mL / m in the planting groove 2 Spraying the bacterium solution; soaking the ro...

Embodiment 3

[0061] Put 30-50ml of liquid medium into a shaker flask for activation; take the activated Pseudomonas vesicularis and put it into the liquid medium for fermentation to make a bacterial liquid, put it into a spectrophotometer, and measure the OD600 concentration to be 0.8 -1.5 fermentation is complete. Each liter of liquid medium includes: 15-18g of gelatin peptone, 10-13g of tryptone, 10-13g of potassium sulfate, 1-2g of magnesium chloride, 15-20g of agar, 100-120ml of glycerol, and water.

[0062] Prepare the bacterial liquid at 0.5mL-2mL / m 2 After spraying in the soil to be repaired, turn over the ground to mix the bacterial solution with the surface soil. The ridges are ridged with a distance of 1.2 meters, a ridge width of 1 meter, and a ridge height of 0.3 meters.

[0063] Open a planting groove with a depth of 5-10cm and a width of 10-15cm on the raised field ridge, and spray 0.5mL-2mL / m in the planting groove 2 Spraying the bacterium solution; soaking the roots of t...

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Abstract

The invention discloses a method of promoting phytoremediation of soil nickel pollutionby utilizing pseudomonas vesicularis, wherein the method of promoting soil nickel pollution with phytoremediationby utilizing the pseudomonas vesicularis comprises the following steps of performing fermentation on a pseudomonas vesicularis strain to prepare a bacterium liquid, wherein the OD600 concentration ofthe bacterium liquid is 0.8-1.5; spraying the bacterium liquid in soils to be repaired, and performing ploughing and ridging; forming a groove on a ridged ribbing, spraying the bacterium liquid, andplanting tobacco seedlings; according to conventional management, after periodically harvesting tobacco plants, performing ploughing again, spraying the bacterium liquid, and planting sweet alyssum seeds; and according to conventional management, periodically treating sweet alyssum. The method provided by the invention can repair nickel polluted soils and is high in planting economic benefit at the same time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for promoting phytoremediation of soil nickel pollution by using Pseudomonas vesicularis. Background technique [0002] Wastewater, waste gas, and waste residues discharged during industrial production, and chemical fertilizers, pesticides, and herbicides used in agricultural production may cause soil heavy metal pollution. When the concentration of heavy metals in the soil reaches a certain value, the planted plants absorb heavy metals, and people eat them directly or indirectly, resulting in the accumulation of heavy metals in the body. When the concentration of heavy metals is too high, plants will be stressed by heavy metals and it is difficult to survive. [0003] In the prior art, enrichment plants are used to enrich the heavy metals in the soil in the plants to reduce the heavy metal content in the soil, but the enrichment plants are usually weeds, etc., and the plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10B09C1/105B09C2101/00
Inventor 张卫刘加力刘拥军黄丹
Owner HUNAN XISI ECOLOGY TECH
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