Single pump micro droplet control system and application thereof

Abstract

The invention discloses a single pump micro droplet control system and application thereof. The system comprises a sample pool; a sample inlet channel, a sample outlet channel, an interconnected cavity, a flow pump and camera equipment, wherein an inlet end of the sample inlet channel is connected with the sample pool; the interconnected cavity comprises a first storage cavity, a second storage cavity, a pressure joint connector and a droplet generating structure; the inlet end of the first storage cavity and the inlet end of the second storage cavity are connected with the outlet end of the sample inlet channel through the pressure joint connector; the outlet end of the first storage cavity and the outlet end of the second storage cavity are communicated with an inlet of the sample outletchannel through the droplet generating structure; the flow pump is connected with an outlet of the sample outlet channel. According to the system disclosed by the invention, by constructing a commonpressure point, two-phase liquid is enabled to generate droplets under the action of a constant relative pressure value, and the uniformity and the stability of the droplets are improved; the volume and the space of the generated droplets are controlled by controlling the geometrical shape of the droplet generating structure and adjusting the speed of the flow pump; by using the single pump to control, the single pump micro droplet control system has the advantages of simple constituent components, small size, and easiness in operation and carrying.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a technical solution for generating micro-droplets under the control of a single pump, which can be widely used in the fields of biochemical analysis and detection such as PCR reaction and double emulsion generation. Background technique [0002] Microfluidics refers to the science and technology involved in systems that use micropipes (tens to hundreds of microns in size) to process or manipulate tiny fluids (nanoliters to microliters in volume). , microelectronics, new materials, emerging interdisciplinary disciplines of biology and biomedical engineering. [0003] Micro-droplets below 1 μL produced by microfluidic technology have important applications in the fields of rapid and high-throughput detection of pollutants, separation and cultivation of biological samples, and observation of the progress of chemical reactions. The continuous flow T-type / cross-type / coaxial fo...

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Problems solved by technology

[0004] However, the continuous flow droplet control system requires a complex and high-precision pressure / flow control system to ensure a relatively accurate flow rate of the water / oil two-phase, and at least two sets of pressure / flow control systems are required to achieve multi-droplet and Formation control of double emulsion structure

The existing micro-droplet control system puts forward high requirements on the number and precision of the pumps. The large weight and volume of the system are not conducive to portability. The volume accuracy and flow accuracy of micro-droplets are not well controlled, which affects the microfluidic control popularization of technology

[0005] Aiming at the shortcomings of the continuous flow droplet control system, such as complex droplet controllability, and poor portability, there is an urgent need for a micro-droplet control system solution with simple system configuration, fewer pumps required, low precision, and precise and controllable droplets.

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