A molecular marker for esophageal cancer and uses thereof

Abstract

A molecular marker for esophageal cancer is disclosed and is an FXR1 gene and / or an expression product of the FXR1 gene. The FXR1 gene is significantly highly expressed in esophageal cancer tissue, and can be adopted as an esophageal cancer molecular marker for esophageal cancer diagnosis. The highly expressed FXR1 gene is closely related to poor prognosis and distant metastasis of patients with esophageal cancer so that the FXR1 gene can be adopted as a prognosis marker for esophageal cancer and can provide effective information for esophageal cancer distant metastasis, prognosis evaluation and treatment effect monitoring. An expression inhibitor of the FXR1 gene is disclosed by the invention and includes siRNA and / or shRNA, has good jamming effects when being used for suppressing FXR1 gene expression and has a potential for application in clinical genetic treatment. The invention further discloses a cell strain capable of stable knock-down of the FXR1 gene. The cell strain is an esophageal cancer cell capable of stable low expression of the FXR1 gene, thus facilitating further research of functions of the FXR1 gene.

Description

technical field [0001] The invention relates to the field of tumor molecular biology, in particular to a molecular marker and application of esophageal cancer, a primer set for diagnosing esophageal cancer, an expression inhibitor of FXR1 gene and a cell line stably knocking down FXR1 gene. Background technique [0002] Esophageal cancer (Esophageal carcinoma, EC) is one of the most common malignant tumors in humans, and more than 400,000 people die of esophageal cancer every year in the world. China is a country with a high incidence of esophageal cancer and one of the countries with the highest mortality rate. The number of deaths from esophageal cancer accounts for almost half of the total number of deaths from esophageal cancer in the world. According to the latest cancer epidemiological data, the incidence of esophageal cancer in my country ranks third among all kinds of malignant tumors in men, fifth in women, and fourth in both men and women in terms of mortality. Du...

AI Technical Summary

Problems solved by technology

In terms of contrast, X-ray barium meal contrast is currently the most commonly used and basic imaging method for displaying esophageal cancer lesions in clinical practice. It can determine the upper and lower ranges of the tumor. However, this method is prone to missed diagnosis in the early detection of esophageal cancer.

The esophageal net method is simple, practical, and highly accurate for the detection of exfoliated cells. It is the primary method for census in areas with a high incidence of esophageal cancer in my country. However, this method is more painful for patients and difficult to be widely used.

Endoscopic examination can directly observe cancerous tumors, detect esophageal mucosal changes more intuitively, and can take tissue for pathological examination. It is the main diagnostic method for esophageal cancer today. However, endoscopic examination is likely to cause adverse effects such as foreign body sensation and nausea in patients. response, and the examination time is long, the patient is not easy to accept

In terms of imaging, although imaging has been widely used in recent years, such as CT, EUS, PET, etc., these often have certain trauma and limitations, and it is easy to miss diagnosis for early cancer diagnosis, and the price is high. Make its application value greatly reduced

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