Capparis masaikal Levl component having sweet taste regulating effect and preparation method and application thereof
A technology for adjusting sweetness and cape nut, which is applied in the fields of non-sugar sweetener-containing food ingredients, organic chemistry, food science, etc., and can solve the problems of bad taste, carcinogenicity, and high concentration
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Embodiment 1
[0021] The preparation method of the composition with regulating sweetness effect in caper nut, comprises the following steps:
[0022] (1) Dried caper nut fruit, peeled off the husk and seed coat, and crushed to obtain seed kernel powder;
[0023] (2) Extract the seed kernel powder with 70% ethanol-water solution by volume for 24 hours, the ratio of solid to liquid is 1g:20mL, extract by cold soaking, combine the extracts, filter, concentrate under reduced pressure to recover ethanol, and obtain dry leaching of caper nuts cream extract;
[0024] (3) Dissolve the extract of betel nut dry extract in distilled water and extract with petroleum ether to obtain ether phase and water phase;
[0025] (4) Concentrate the water phase and use a gel column to refine and remove impurities. The eluent is a mixture of water and ethanol with a volume ratio of 3:1. The obtained eluent is purified by macroporous resin, and isocratic elution. The stripping agent is a mixture of water and etha...
Embodiment 2
[0028] The preparation method of the composition with regulating sweetness effect in caper nut, comprises the following steps:
[0029] (1) Dried caper nut fruit, peeled off the husk and seed coat, and crushed to obtain seed kernel powder;
[0030] (2) Extract the seed kernel powder with 80% ethanol-water solution by volume for 36 hours, the ratio of solid to liquid is 1g:30mL, extract by cold soaking, combine the extracts, filter, concentrate under reduced pressure to recover ethanol, and obtain the dry leaching of betel nut cream extract;
[0031] (3) Dissolve the extract of betel nut dry extract in distilled water and extract with petroleum ether to obtain ether phase and water phase;
[0032] (4) Concentrate the water phase and use a gel column to refine and remove impurities. The eluent is a mixture of water and ethanol with a volume ratio of 6:1. The stripping agent is a mixture of water and ethanol with a volume ratio of 10:1, and the eluate is dried to obtain the swe...
Embodiment 3
[0035] The preparation method of the composition with regulating sweetness effect in caper nut, comprises the following steps:
[0036] (1) Dried caper nut fruit, peeled off the husk and seed coat, and crushed to obtain seed kernel powder;
[0037] (2) Extract the seed kernel powder with 90% ethanol-water solution by volume for 48 hours, the ratio of solid to liquid is 1g:40mL, extract by cold soaking, combine the extracts, filter, concentrate under reduced pressure to recover ethanol, and obtain the dry immersion of caper nut cream extract;
[0038] (3) Dissolve the extract of betel nut dry extract in distilled water and extract with petroleum ether to obtain ether phase and water phase;
[0039] (4) Concentrate the water phase and use a gel column to refine and remove impurities. The eluent is a mixture of water and ethanol with a volume ratio of 10:1. The deagent is a mixture of water and ethanol with a volume ratio of 20:1, and the eluent is dried to obtain the sweetness...
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