Preparation method of injectable chitosan-oyster shell hydroxyapatite temperature-sensitive hydrogel

Abstract

The invention discloses a preparation method of injectable chitosan-oyster shell hydroxyapatite temperature-sensitive hydrogel. The preparation method comprises: preparing a chitosan acetate solutionand a sodium beta-glycerol phosphate solution, putting 9ml of the chitosan acetate solution into a beaker, putting the beaker into a constant temperature stirring water bath pot, carrying out stirringat a middle rate under the action of a magnetic force for 5min, simultaneously, slowly adding 2.5% of oyster shell nano-hydroxyapatite powder into the solution, carrying out stirring for 0.5h to obtain a hydrogel stock solution, treating the hydrogel stock solution and the sodium beta-glycerol phosphate solution in an ice bath for 15min, adding 1ml of the sodium beta-glycerol phosphate solution treated by the ice bath into the hydrogel stock solution drop by drop, and carrying out stirring in the constant temperature stirring water bath pot at 30 DEG C at a middle stirring rate under the action of a magnetic force for 10min to obtain the injectable chitosan-oyster shell hydroxyapatite temperature-sensitive hydrogel. The hydrogel has the characteristics of injectability, temperature sensitivity, safety and non-toxicity and can be used as a bone repair material.

Description

technical field [0001] The invention relates to a preparation method of an injectable chitosan oyster shell hydroxyapatite temperature-sensitive hydrogel. Background technique [0002] At present, bones will crack, break or break under the influence of diseases, car accidents and sports trauma. Trauma or disease of bone or cartilage tissue is usually accompanied by structural defects, especially in the modern fast-paced life, so The bone repair and bone replacement materials with social benefits in the human body have gradually gained the attention of researchers. At present, there are mainly three methods for the clinical treatment of bone defects, namely allograft bone grafting, autologous bone grafting and the use of metal substitute materials. For allogeneic bone grafts from a wide range of sources, the main defect is that it is prone to immune rejection or disease transmission, and the source of autologous bone grafts without these defects is limited, and will cause ne...

AI Technical Summary

Problems solved by technology

For allogeneic bone grafts from a wide range of sources, the main defect is that it is prone to immune rejection or disease transmission, and the source of autologous bone grafts without these defects is limited, and will cause new local lesions, which cannot be replaced by the body's own tissues. Metal materials that are transplanted into the human body will experience fatigue and fracture.

These methods not only require surgery, but also bring too much pain and even sequelae to patients after transplantation. Therefore, it is of great significance to find bone repair materials that are safe, non-toxic, effective, and easy to transplant.