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Preparation method of eucommia ulmoides leaf extract

A technology of eucommia leaves and extracts, which is applied in the field of preparation of eucommia leaves extracts, can solve the problems of low purity, waste of effective substances, use restrictions, etc., and achieve the effect of wide application range and high purity

Inactive Publication Date: 2018-12-14
维康腾达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction means of the prior art, the extraction rate of extracting chlorogenic acid and flavonoids is not high, resulting in waste of effective substances, and the purity is not high, so it is limited in use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of eucommia leaf extract, comprising the following steps:

[0022] (1) Raw material processing: Weigh clean and dry eucommia leaves, pulverize them in a closed Chinese herbal medicine grinder (FW135 type), pass through a 40-80 mesh screen in a vibrating sieving machine, and collect eucommia leaf powder for future use;

[0023] (2) Pretreatment: Weigh eucommia leaf powder, add 40%-60% mass fraction of ethanol solution, the ratio of liquid to material is (10-16) ml: 1g, and carry out infiltration pretreatment, and the temperature in the water bath is 40-50 Add cellulase at ℃, stir for 5-10 minutes, then raise the temperature to 50-60℃, add pectinase, stir for 5-10 minutes;

[0024] (3) Extraction: Reflux extraction of the pretreated liquid-solid mixture, the extraction temperature is 60-95°C, the stirring speed is 600-1000r / min, the extraction time is 0.5-2h, and the number of extractions is 2-4 times. After standing for several hours, take the super...

Embodiment 2

[0036] A preparation method of eucommia leaf extract, comprising the following steps:

[0037] (1) Raw material processing: Weigh clean and dry Eucommia leaves, pulverize them in a closed Chinese herbal medicine grinder (FW135 type), pass through a 40-mesh sieve in a vibrating sieving machine, and collect Eucommia leaf powder for future use;

[0038] (2) Pretreatment: Weigh eucommia leaf powder, add 60% ethanol solution with a mass fraction of 10ml:1g, carry out infiltration pretreatment, add cellulase in a water bath at a temperature of 50°C, and stir for 5 minutes , then warming up to 60°C, adding pectinase, and stirring for 5 minutes;

[0039] (3) Extraction: Reflux extraction of the pretreated liquid-solid mixture, the extraction temperature is 60°C, the stirring speed is 1000r / min, the extraction time is 0.5h, and the number of extractions is 2 times. After standing for several hours, take the upper layer supernatant, and combined several times the upper layer extract; ...

Embodiment 3

[0051] A preparation method of eucommia leaf extract, comprising the following steps:

[0052] (1) Raw material processing: Weigh clean and dry eucommia leaves, pulverize them in a closed Chinese herbal medicine grinder (FW135 type), pass through an 80-mesh sieve in a vibrating sieving machine, and collect eucommia leaf powder for future use;

[0053] (2) Pretreatment: Weigh eucommia leaf powder, add 40% ethanol solution with a mass fraction of 16ml:1g, carry out infiltration pretreatment, add cellulase in a water bath at a temperature of 40°C, and stir for 10 minutes , then warming up to 50°C, adding pectinase, and stirring for 10 minutes;

[0054] (3) Extraction: Reflux extraction of the pretreated liquid-solid mixture, the extraction temperature is 95°C, the stirring speed is 600r / min, the extraction time is 2h, and the number of extractions is 4 times. After standing for several hours, take the supernatant solution, and combined several times of the upper layer extract; ...

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Abstract

The invention provides a preparation method of a eucommia ulmoides leaf extract. The method comprises the following steps: (1) raw material processing; (2) pretreatment; (3) extraction; (4) impurity removal; (5) separation and purification: 1) macroporous resin adsorption desorption; 2) ultrafiltration membrane filtration; and 3) nanofiltration membrane concentration; (6) concentration and drying;The method does not use toxic and harmful solvent for extraction, and increases an use range of the eucommia ulmoides leaf extract, and the method improves the purity of the product and increases theyield through the steps of resin adsorption desorption and membrane separation and filtration.

Description

technical field [0001] The invention belongs to the field of plant natural product extraction, and in particular relates to a preparation method of Eucommia ulmoides leaf extract. Background technique [0002] Eucommia, also known as Bakelite, is a plant of Eucommiaceae, which is rich in chlorogenic acid and flavonoids. Chlorogenic acid, also known as coffee tannin, is a chemical substance extracted from plants. Chlorogenic acid is an important biologically active substance that can inhibit bacteria, viruses, increase white blood cells, protect liver and gallbladder, prevent tumors, lower blood pressure, Blood lipids, scavenging free radicals and stimulating the central nervous system; flavonoids also play an important role in antibacterial and disease prevention. The extraction method of the prior art has a low extraction rate of chlorogenic acid and flavonoids, resulting in waste of effective substances, and the purity is not high, so the use is limited. Contents of the...

Claims

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Application Information

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IPC IPC(8): A61K36/46A61K8/9789A61Q19/00A61K127/00
CPCA61K8/9789A61K36/46A61K2236/19A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61K2800/10A61Q19/00
Inventor 周维辉刘升军文欣伟贾合银齐百涛周维智韩晓利陈发刚
Owner 维康腾达生物科技有限公司
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