Preparation method and application of bovine spinal cord peptide

A technology of bovine spinal cord and spinal cord, applied in the field of preparation of bovine spinal cord peptide, can solve the problems of not necessarily good curative effect and great harm to human body, etc.

Active Publication Date: 2018-12-07
JILIN UNIV
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, radiation therapy and surgery are mainly used for syringomyelia, but radiation therapy is very harmfu...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of bovine spinal cord peptide, comprising the following steps

[0033] (1) After the frozen bovine spinal cord is thawed at room temperature, it is broken into 3 cm spinal cord pieces and added with distilled water twice the weight and volume, that is, 100 grams of bovine spinal cord tissue is added with 200 ml of distilled water, and cooked at 100°C for 30 minutes.

[0034] (2) Tissue homogenization: Homogenize the above-mentioned cooked spinal cord tissue, add distilled water according to the mass volume ratio of spinal cord and distilled water at 2:1 during homogenization, that is, add 50 ml of distilled water to 100 grams of bovine spinal cord tissue, and the homogenization time is 10 minute.

[0035] (3) Degreasing: the above-mentioned bovine spinal cord homogenate tissue is degreased by a degreasing agent to obtain a degreasing material. The degreasing agent is petroleum ether, and 200 milliliters of petroleum ether is added to the bovine spin...

Embodiment 2

[0041] (1) After the frozen bovine spinal cord is thawed at room temperature, it is broken into 3 cm spinal cord pieces and added with distilled water twice the weight and volume, that is, 100 grams of bovine spinal cord tissue is added with 200 ml of distilled water, and cooked at 100°C for 30 minutes.

[0042] (2) Tissue homogenization: Homogenize the above-mentioned cooked spinal cord tissue, add distilled water according to the mass volume ratio of spinal cord and distilled water at 2:1 during homogenization, that is, add 50 ml of distilled water to 100 grams of bovine spinal cord tissue, and the homogenization time is 10 minute.

[0043] (3) Degreasing: the above-mentioned bovine spinal cord homogenate tissue is degreased by a degreasing agent to obtain a degreasing material. The degreasing agent is petroleum ether, and 200 milliliters of petroleum ether is added to the bovine spinal cord homogenate tissue of every 100 grams, set every 4 hours Replace the degreasing agent...

Embodiment 3

[0049] (1) After thawing the frozen bovine spinal cord at room temperature, break it into 3 cm spinal cord pieces and add 2 times the mass volume of distilled water, that is, 100 grams of bovine spinal cord tissue plus 200 ml of distilled water, cook at 100 ° C for 30 minutes

[0050] (2) Tissue homogenization: Homogenize the above-mentioned cooked spinal cord tissue, add distilled water according to the mass volume ratio of spinal cord and distilled water at 2:1 during homogenization, that is, add 50 ml of distilled water to 100 grams of bovine spinal cord tissue, and the homogenization time is 10 minute

[0051] (3) Degreasing: the above-mentioned bovine spinal cord homogenate tissue is degreased by a degreasing agent to obtain a degreasing material. The degreasing agent is petroleum ether, and 200 milliliters of petroleum ether is added to the bovine spinal cord homogenate tissue of every 100 grams, set every 4 hours Replace the degreasing agent once, a total of 6 times, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of bovine spinal cord peptide. The preparation method comprises the steps of unfreezing frozen bovine spinal cord at a room temperature, and carrying out high-temperature cooking, tissue homogenization, degreasing, enzymolysis, ultrafiltration, freeze drying or spray drying, so as to obtain bovine spinal cord peptide powder. Spinal cord is a part of a central nervous system of human or a vertebrate, the upper end of the spinal cord is connected with a medulla, paired nerves grow on two sides of the spinal cord, and an H-shaped grey matter region isformed in the spinal cord; and the spinal cord is a passage between the nerves and the brain and a low-level pivot for the many simple reflection activities, so that the spinal cord is a very specialtissue organ of the vertebrate and necessarily contains special functional factors according to a traditional Chinese medicine theory of reinforcing organs with organs. An analytical detection resultshows that fresh spinal cord contains nutrient substances, macroelements, microelements, fatty acids and various amino acids required by a human body, so that the spinal cord has a very high nutritional value. Prepared bovine spinal cord nutritional whole powder and prepared spinal cord polypeptide powder can be used for developing common foods and health products or can be applied to the medicinefield.

Description

technical field [0001] The invention relates to a preparation method and application of bovine spinal cord peptide, belonging to the field of biotechnology. It is a polypeptide prepared by hydrolysis using bovine spinal cord as a raw material, and has a good application prospect in the fields of food, health products and pharmaceuticals Background technique [0002] With the development of economic level, transportation industry and construction industry, the incidence of spinal cord injury is increasing year by year. Spinal cord injury is a serious trauma to the central nervous system, which can cause severe disability and partial neurological dysfunction, and spinal cord injury is the most serious complication of spinal cord injury, often leading to severe dysfunction of the limbs below the injured segment At present, the treatment for spinal cord injury mainly includes: physical therapy, surgical treatment and western medicine treatment. The effect of physical therapy i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P21/06C07K2/00C07K1/34A23L33/18A23L29/00A61K38/01A61P19/00
CPCA23V2002/00A61K38/00A23L29/045A23L33/18A61P19/00C07K2/00C12P21/06A23V2200/30A23V2250/55
Inventor 叶海青柳慧琴张铁华
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap