Boletus aereus strain and domestication method thereof
A technology of black boletus and strain, which is applied in the field of biological agriculture and achieves the effects of simple and fast processing method, strong strain stability, and stable fruiting and yield.
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Embodiment 1
[0080] The present embodiment provides a black boletus strain, which is prepared by the following domestication method:
[0081] S1, cultivating fruiting bodies based on pomelo tree as host tree under low temperature environment and collecting;
[0082] S2, cutting the junction of the stipe and the cap of the fruiting body to obtain a piece of bacterial flesh tissue with length×width×thickness=0.5cm×0.5cm×0.3cm;
[0083] S3, the culture medium of the bacterial meat tissue block was cultured at 25° C. for 20 days in the dark to obtain the parent species.
[0084] S4, expanding the parent species to obtain liquid strains;
[0085] S5, inoculating the liquid bacteria in the first culture container at 26°C under dark light conditions, and culturing for 30 days;
[0086] S6, covering the mycelium with soil in the culture container, and culturing at 27° C. for 10 days to obtain the soil-covered mycelium that has entered the Cuilei stage.
[0087] S7, inoculating the soil-covered ...
Embodiment 2
[0095] The present embodiment provides a black boletus strain, which is prepared by the following domestication method:
[0096] S1, cultivating and collecting fruiting bodies based on gardenia as a host tree in a low temperature environment;
[0097] S2, cutting the junction of the stipe and the cap of the fruiting body to obtain a piece of bacterial flesh tissue with length×width×thickness=0.5cm×0.5cm×0.3cm;
[0098] S3, the culture medium of the bacterial meat tissue pieces was cultured at 26° C. in the dark for 25 days to obtain the parent species.
[0099] S4, expanding the parent species to obtain liquid strains;
[0100] S5, inoculating the liquid bacteria in the first culture container at 27°C under dark light conditions, and culturing for 30 days;
[0101] S6, covering the mycelium with soil in the culture container, and culturing at 28° C. for 10 days to obtain the soil-covered mycelium that has entered the Cuilei stage.
[0102] S7, inoculating the soil-covered m...
Embodiment 3
[0110] The present embodiment provides a black boletus strain, which is prepared by the following domestication method:
[0111] S1, cultivating and collecting fruiting bodies based on coffee trees as host trees in a low temperature environment;
[0112] S2, cutting the junction of the stipe and the cap of the fruiting body to obtain a piece of bacterial flesh tissue with length×width×thickness=0.5cm×0.5cm×0.3cm;
[0113] S3, the culture medium of the bacterial meat tissue block was cultured at 28° C. in the dark for 30 days to obtain the parent species.
[0114] S4, expanding the parent species to obtain liquid strains;
[0115] S5, inoculating the liquid bacteria in the first culture container at 28°C under dark light conditions, and culturing for 30 days;
[0116] S6, covering the mycelium with soil in the culture container, and culturing at 29° C. for 10 days to obtain the soil-covered mycelium that has entered the Cui Lei stage.
[0117] S7, inoculating the soil-covere...
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