Application of DCMU in stabilizing polyculture growth pH of microalgae or increasing polyculture growth speed of microalgae

A growth rate, microalgae technology, applied in the field of microalgae biology, can solve the problems of reducing the growth rate of microalgae, the pH fluctuation of the medium, and complicated operations, and achieve the effects of promoting the growth rate of microalgae, low concentration, and easy operation.

Active Publication Date: 2022-03-18
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the growth of microalgae using organic matter will cause large fluctuations in the pH of the medium, and thus reduce the growth rate of microalgae
Although methods such as adding pH buffers and artificially adjusting the pH of the fermentation medium can stabilize the pH to a certain extent and increase the growth rate of microalgae, they cannot be applied at the industrial level due to problems such as high cost and cumbersome operation.

Method used

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  • Application of DCMU in stabilizing polyculture growth pH of microalgae or increasing polyculture growth speed of microalgae
  • Application of DCMU in stabilizing polyculture growth pH of microalgae or increasing polyculture growth speed of microalgae
  • Application of DCMU in stabilizing polyculture growth pH of microalgae or increasing polyculture growth speed of microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Take Pseudochlorella kelvinia ( Parachlorella kessleri ) FACHB-4 (purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences) is a microalgae species. First, it was enriched and cultured with BG11 culture medium. 8 ~1×10 9 Pseudochlorella kelvinia seed solution was obtained at the time of individual / mL. The composition of BG11 culture medium is: NaNO 3 1.5 g / L, K 2 HPO 4 40 mg / L, MgSO 4 ·7H 2 O 70 mg / L, CaCl 2 2H 2 O 40 mg / L, citric acid 6 mg / L, ferric ammonium citrate 6 mg / L, EDTA Na 2 1 mg / L, Na 2 CO 3 20 mg / L, H 3 BO 3 2.86 mg / L, MnCl 2 4H 2 O 1.86 mg / L, ZnSO 4 ·7H 2 O 0.22 mg / L, Na 2 MoO 4 2H 2 O 0.39 mg / L, CuSO 4 ·5H 2 O 0.08 mg / L, Co(NO 3 ) 2 ·6H 2 O 0.05 mg / L, pH=7.00.

[0029] (2) Configure the microalgae glucose polyculture medium, the medium is BG11 medium plus 2 g / L glucose, pH=7.00.

[0030] (3) Inoculate the seed solution of Pseudochlorella kejeldahl into the microalgae-glucose mixed culture mediu...

Embodiment 2

[0038] (1) Take Pseudochlorella kelvinia ( Parachlorella kessleri ) FACHB-4 (purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences) is a microalgae species. First, it was enriched and cultured with BG11 culture medium. 8 ~1×10 9 Pseudochlorella kelvinia seed solution was obtained at the time of individual / mL. The composition of BG11 culture medium is: NaNO 3 1.5 g / L, K 2 HPO 4 40 mg / L, MgSO 4 ·7H 2 O 70 mg / L, CaCl 2 2H 2 O 40 mg / L, citric acid 6 mg / L, ferric ammonium citrate 6 mg / L, EDTA Na 2 1 mg / L, Na 2 CO 3 20 mg / L, H 3 BO 3 2.86 mg / L, MnCl 2 4H 2 O 1.86 mg / L, ZnSO 4 ·7H 2 O 0.22 mg / L, Na 2 MoO 4 2H 2 O 0.39 mg / L, CuSO 4 ·5H 2 O 0.08 mg / L, Co(NO 3 ) 2 ·6H 2 O 0.05 mg / L, pH=7.00.

[0039] (2) Configure the microalgae glucose polyculture medium, the medium is BG11 medium plus 2 g / L glucose, pH=7.00.

[0040] (3) Inoculate the seed liquid of Pseudochlorella kesperi into the microalgae-glucose mixed culture medium at...

Embodiment 3

[0048] (1) Take Pseudochlorella kelvinia ( Parachlorella kessleri ) FACHB-4 is a species of microalgae, which is enriched and cultivated with BG11 culture medium first, when the cell density of Pseudochlorella kescheri reaches 1×10 8 ~1×10 9 Pseudochlorella kelvinia seed solution was obtained at the time of individual / mL. The composition of BG11 culture medium is: NaNO 3 (1.5 g / L), K 2 HPO 4 (40 mg / L), MgSO 4 ·7H 2 O (70 mg / L), CaCl 2 2H 2 O (40 mg / L), citric acid (6 mg / L), ferric ammonium citrate (6 mg / L), EDTA Na 2 (1mg / L), Na 2 CO 3 (20 mg / L), H 3 BO 3 (2.86 mg / L), MnCl 2 4H 2 O (1.86 mg / L), ZnSO 4 ·7H 2 O (0.22 mg / L), Na 2 MoO 4 2H 2 O (0.39 mg / L), CuSO 4 ·5H 2 O (0.08 mg / L), Co(NO 3 ) 2 ·6H 2 O (0.05 mg / L), pH=7.00.

[0049] (2) Configure microalgae polyculture medium with different glucose content, the medium is BG11 medium plus 3 g / L, 4 g / L, 5 g / L glucose, pH=7.00.

[0050] (3) Inoculate the seed liquid of Pseudochlorella kesperi into micro...

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Abstract

The invention discloses application of DCMU in stabilizing the polyculture growth pH of microalgae or increasing the polyculture growth speed of the microalgae. According to the invention, the DCMU is found to have the effects of stabilizing the growth pH and promoting the growth speed of microalgae in microalgae polyculture for the first time. The invention provides a method for efficiently increasing the polyculture growth speed of microalgae. The DCMU is very cheap and extremely low in use concentration, and almost no extra industrial cost is generated, so that the method provided by the invention is very suitable for industrial culture of microalgae; the method provided by the invention has the characteristics of simplicity and convenience in operation and high practicability, and is beneficial to large-scale industrial application.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and particularly relates to the application of DCMU in stabilizing the growth pH of microalgae polyculture or increasing the growth rate of microalgae polyculture. Background technique [0002] Microalgae is a general term for a class of single-celled photosynthetic micro organisms. Microalgae have emerged as an attractive alternative to traditional terrestrial plants for the production of biofuels and other high-value products while alleviating pressure on arable land, carbon dioxide emissions, and global warming. However, achieving high productivity of microalgal products remains a major challenge for the commercial application of microalgae. The polyculture fermentation of microalgae refers to a culture mode in which microalgae use organic matter and light to grow at the same time. Due to the advantages of both photoautotrophic growth and heterotrophic growth, polyculture fermentation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 刘芳华谢章彰王欧美汤佳郝钦钦
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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