Gallbladder cancer diagnostic and prognostic marker pim1

A technique for gallbladder cancer and prognosis, applied in the field of biomedicine, can solve the problems of unclear expression level and functional role

Active Publication Date: 2021-08-27
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the expression level and functional role of PIM1 in gallbladder carcinoma are unclear, and more studies are needed

Method used

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  • Gallbladder cancer diagnostic and prognostic marker pim1
  • Gallbladder cancer diagnostic and prognostic marker pim1
  • Gallbladder cancer diagnostic and prognostic marker pim1

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention is explained below through several typical experiments, but the present invention is not limited thereto.

[0023] 1. Western blot detection of PIM1 protein expression in gallbladder carcinoma tissues and cells

[0024] Experiment 1.1:

[0025] Nine cases of gallbladder cancer tissues preserved in liquid nitrogen were added to the tissue protein lysate, thoroughly ground on ice with an electric homogenizer, placed on an ice shaker for 1 h, centrifuged at 10,000 r / min at 4°C for 15 min, and the supernatant was taken. The protein concentration was detected with a BCA microplate reader, and the protein was adjusted and added to the buffer and boiled at 100°C for 5 minutes. Protein samples were added to prefabricated 12% polyacrylamide gel swimming lanes, and electrophoresis was performed at 110V for 90 minutes. After the protein electrophoresis is completed, it is transferred to the nitrocellulose membrane, and the membrane is placed in PBST solutio...

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PUM

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Abstract

The invention discloses gallbladder cancer diagnosis and prognosis judgment marker PIM1, and also discloses a gallbladder cancer diagnosis and prognosis judgment kit, which can be used for diagnosis, prediction, detection or screening of human gallbladder cancer cell proliferation, gallbladder cancer clinical stage or gallbladder cancer cancer patient prognosis. The invention also discloses the use of the PIM1 protein inhibitor in the preparation of anti-gallbladder cancer drugs. The present invention shows that PIM1 has very important significance in clinical practice, and may become a new generation of tumor biodiagnostic markers and molecular targets for tumor treatment, and opens up the research of new targets for gallbladder cancer mainly based on PIM1. Open up a new way for the development of new anti-tumor drugs.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to gallbladder cancer markers. Background technique [0002] Gallbladder cancer (GBC for short) is the most common form of malignant biliary tract tumors, accounting for 80%-95% of malignant biliary tract tumors. In the past few decades, no substantial progress has been made in the treatment of gallbladder cancer, and due to the lack of typical symptoms, the early diagnosis rate is only 19.1%, and most patients are already in the middle and late stages when they are clearly diagnosed. So far, surgical resection is still the main treatment for patients with gallbladder cancer, but surgical resection is not suitable for advanced patients. Gallbladder cancer is not sensitive to radiotherapy, chemotherapy and other treatment measures, so the overall prognosis is extremely poor. Foreign epidemiological studies have shown that the 5-year survival rate of gallbladder cancer after surg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50A61K45/00A61P35/00
CPCA61P35/00A61K31/5025A61K31/713A61P35/04G01N33/6854A61K2300/00
Inventor 何玉婷薛晨孙冉冉任志刚史青苗孙姜华肖楠余祖江
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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