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Fixing treatment method of tumor lymph node specimen in vitro

A technology of isolated specimens and processing methods, which is applied in the field of test sample preparation, can solve the problems affecting the patient's treatment plan, prognosis and outcome, insufficient material collection, long operation time, etc., achieve accurate positive rate of lymph node metastasis, improve detection The effect of producing numbers and conveniently obtaining materials

Inactive Publication Date: 2018-11-30
SHAOXING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the ex vivo tissue specimens of tumor lymph node dissection, thorough lymph node sampling is very important for tumor diagnosis and staging, formulation of treatment plans, and judgment of prognosis. However, neither conventional fixative solution nor existing specimen fixation methods can meet the requirements of rapid fixation while improving the quality of lymph nodes. The speed and detection rate of material collection make pathologists find lymph nodes in the long-term isolated adipose tissue with many disadvantages such as insufficient material collection and long operation time, which will ultimately affect the patient's treatment plan, prognosis and outcome

Method used

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  • Fixing treatment method of tumor lymph node specimen in vitro
  • Fixing treatment method of tumor lymph node specimen in vitro
  • Fixing treatment method of tumor lymph node specimen in vitro

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: different compositions of ointment fixative

[0035] The core functional components of the palmitate fixative solution provided by this application are mainly formaldehyde, lecithin, absolute ethanol and acetone, and in different implementation environments, deoxycholic acid, methanol, PBS solution and any One or more, the specific composition selection method can refer to Table 1.

[0036] Table 1 different compositions of ointment fixative

[0037]

[0038]

[0039] We tested the core components of the ointment fixative (i.e. No. 1 in Table 1) and supplementary components respectively, and the above 16 situations can be obtained. The same components were tested in parallel with the same samples at the same concentration, and the results showed that: The soft lipid fixative of the core component has a good fixation effect on adipose tissue, organ tissue (such as thyroid tissue, breast tissue, lung tissue) or colon tissue, and the lymph nodes are we...

Embodiment 2

[0042] Embodiment 2: the same composition different concentrations of ointment fixed solution

[0043] The present embodiment provides the composition and concentration of the palmitate fixative solution of the whole composition (i.e. including all core constituents and all supplementary constituents), and several representative groups are listed as shown in Table 2:

[0044] Table 2 different concentrations of palmitate fixative

[0045]

[0046]

[0047] The pretreated surgically resected omentum specimens were treated with the omentum fixative solution corresponding to the serial numbers in Table 2 for 4 hours, and then the lymph nodes were collected, dehydrated, embedded, sectioned, stained, immunohistochemical, and observed under a microscope. The results showed that: The above-mentioned concentrations can dissolve the sample to varying degrees, the fat is soft and liquefied, the lymph nodes are effectively exposed, the cell morphology of the lymph nodes is preserve...

Embodiment 3

[0048] Embodiment 3: Digestive juice of different compositions constitutes

[0049] The core functional components of the digestive juice provided by this application are digestive enzymes, and the main components are proteinase K, trypsin and EDTA. Under different implementation environments, one of PBS or Tris-HCl can also be used as a buffer, and a CaCl 2 To stabilize the enzyme activity, the specific composition selection method can be seen in Table 3.

[0050] Table 3 Digestive fluids with different compositions

[0051]

[0052]

[0053] We tested the digestive juice of the core components (ie, No. 1 in Table 3) and the supplementary components respectively, and obtained the above 8 situations. The same components were tested in parallel with the same samples at the same concentration, and the results showed that: the core components The main function of the ingredients is to destroy the fat cell membrane and increase the permeability of the fat cell membrane. H...

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Abstract

The invention relates to a fixing treatment method of tumor lymph node specimen in vitro and belongs to the technical field of detection specimen preparation. The method includes: performing fat softening treatment to an in-vitro specimen in a stationary liquid, performing adipocyte permeability enhancement treatment, and treating the specimen at constant temperature 35-39 DEG C for 2-6 h with a digestion liquid. The fat softening treatment is carried out by soaking the specimen at room temperature in the stationary liquid for 2-24 h. The immobilizing liquid includes formaldehyde, lecithin, anhydrous ethanol and acetone according to the concentrations of 50-150 ml / L of the formaldehyde, 30-70 g / L of the lecithin, 200-300 ml / L of the anhydrous ethanol and 200-300 ml / L of the acetone. The treatment method is applied to treatment on tumor lymph node specimen in vitro, and can increase the detection number of lymph nodes and has accuracy in lymphatic metastasis positive rate.

Description

technical field [0001] The application relates to a method for fixing and processing tumor lymph node isolated specimens, and belongs to the technical field of test sample preparation. Background technique [0002] Existing tumor lymph node dissected tissue specimens in vitro are usually soaked in formalin fixative solution, mainly because formaldehyde can preserve histomorphological and structural characteristics for a long time and has good economic utility at the same time. Although this type of specimen processing method can well fix the shape of the tissue, it also hardens the specimen containing adipose tissue, which greatly increases the difficulty in obtaining materials in pathology departments, especially lymph nodes. In the ex vivo tissue specimens of tumor lymph node dissection, thorough lymph node sampling is very important for tumor diagnosis and staging, formulation of treatment plans, and judgment of prognosis. However, neither conventional fixative solution n...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/28
CPCG01N1/28G01N1/30G01N2001/305
Inventor 陈洋张振华俞樟森焦青川张剑孙爱静
Owner SHAOXING UNIVERSITY
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