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Fermentation agent for degrading aflatoxin, and preparation method and application thereof

A kind of aflatoxin and fermentation agent technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of incomplete detoxification, strict reaction conditions, uncontrollable degradation effect, etc., and improve intestinal tract Bacteria and microenvironment, enhance digestion and absorption function, improve the effect of bioavailability

Active Publication Date: 2018-11-30
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional physical and chemical methods are used to treat aflatoxin-contaminated agricultural products or feed; however, these methods have problems such as incomplete detoxification, reduced nutritional value, high cost, and poor applicability
In recent years, biological control of aflatoxin pollution has become a research hotspot. Many microorganisms that inhibit the growth of Aspergillus flavus, inhibit the production of toxins by Aspergillus flavus and degrade aflatoxin have been isolated and cultivated; however, most of these microorganisms are limited to the laboratory research stage , there are problems such as strict reaction conditions, large differences in the degradation rate of Aspergillus flavus, and uncontrollable degradation effects in actual industrial applications.

Method used

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  • Fermentation agent for degrading aflatoxin, and preparation method and application thereof
  • Fermentation agent for degrading aflatoxin, and preparation method and application thereof
  • Fermentation agent for degrading aflatoxin, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A fermenting agent for degrading aflatoxin is composed of a compound microbial bacterial agent and a synergist according to a weight-to-volume ratio of 4:1;

[0037] The composite microbial agent is formed by mixing Streptococcus thermophilus, Bifidobacterium, Candida utilis and Bacillus licheniformis according to the weight ratio of 1:1:3:3.

[0038] The synergist is prepared by mixing plant essential oils and auxiliary agents in a volume ratio of 1:0.3.

[0039] The plant essential oil is formed by mixing tea tree essential oil and cinnamon essential oil in a volume ratio of 1:1.

[0040] The auxiliary agent is prepared by mixing ethanol, Tween-80 and Span-80 in a volume ratio of 1:1:1.

Embodiment 2

[0042] A fermenting agent for degrading aflatoxin is composed of a compound microbial bacterial agent and a synergist according to a weight-to-volume ratio of 4:1;

[0043] The composite microbial bacterial agent is formed by mixing streptococcus thermophilus, bifidobacterium, candida utilis and bacillus licheniformis according to the weight ratio of 3:3:5:5.

[0044] The synergist is formed by mixing plant essential oils and auxiliary agents in a volume ratio of 1:0.3-0.5.

[0045] The plant essential oil is formed by mixing cinnamon essential oil and litsea cubeba oil in a volume ratio of 1:1.

[0046] The auxiliary agent is formed by mixing glycerol, Tween-80 and Span-80 in a volume ratio of 1:1:1.

Embodiment 3

[0048] A fermenting agent for degrading aflatoxin is composed of a compound microbial bacterial agent and a synergist according to a weight-to-volume ratio of 4:1;

[0049] The composite microbial bacterial agent is formed by mixing Streptococcus thermophilus, Bifidobacterium, Candida utilis and Bacillus licheniformis according to the weight ratio of 5:5:8:8.

[0050] The synergist is prepared by mixing plant essential oils and auxiliary agents in a volume ratio of 1:0.5.

[0051] The plant essential oil is formed by mixing eucalyptus oil and litsea cubeba oil in a volume ratio of 1:1.

[0052] The auxiliary agent is formed by mixing propanol, ethylene glycol, Tween-80 and Span-80 in a volume ratio of 1:1:1:1.

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PUM

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Abstract

The invention discloses a fermentation agent for degrading aflatoxin, and a preparation method and application thereof, and belongs to the technical field of toxin degradation. The fermentation agentfor degrading the aflatoxin is formed by a compound microbial agent and a synergist according to the weight-to-volume ratio of 4:1, and the compound microbial agent is formed by Streptococcus thermophilus, bifidobacterium, candida utilis and bacillus licheniformis which are mixed according to the weight ratio of (1-5):(1-5):(3-8):(3-8). The fermentation agent can effectively degrade the aflatoxinB1 in crops or agricultural products, and a degraded product is safe and does not generate the toxic effect on animal bodies.

Description

technical field [0001] The invention belongs to the technical field of toxin degradation, and in particular relates to a starter for degrading aflatoxin, its preparation method and application. Background technique [0002] Aflatoxins are mainly secondary metabolites produced by Aspergillus flavus (A.flavus) and Aspergillus parasiticus (A.parasiticus), which are derivatives of dihydrofuranocoumarins, and were approved by the World Health Organization (WHO) in 1993. Cancer Research Institute delineated as a class I carcinogen. [0003] Traditionally, physical and chemical methods are used to treat aflatoxin-contaminated agricultural products or feeds; however, these methods have problems such as incomplete detoxification, reduced nutritional value, high cost, and poor applicability. In recent years, biological control of aflatoxin pollution has become a research hotspot. Many microorganisms that inhibit the growth of Aspergillus flavus, inhibit the production of toxins by As...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23L5/20C12R1/46C12R1/72C12R1/10C12R1/01
CPCA23L5/28C12N1/16C12N1/20A23V2400/51A23V2400/249
Inventor 杨庆利常鹏于春娣唐娟赵海燕朱英莲
Owner QINGDAO AGRI UNIV
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