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Greenhouse environment-friendly rapid propagation method of sweet potato detoxification test-tube seedlings

A technology for test-tube seedlings and sweet potatoes, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high economic cost, sweet potato's susceptibility to virus infection, aggravation, etc.

Active Publication Date: 2018-11-30
CROP RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are certain deficiencies in the traditional virus-free breeding technology: first, it takes 3-4 years from virus-free seedlings to production seeds, which is a long cycle; The number of generations increases and increases from generation to generation, and the quality of seed potatoes decreases; again, the manpower and material resources required in the breeding process increase, and the economic cost is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The Jishu 26 detoxified sweet potato test tube seedlings obtained by conventional technical means and tested for viruses were transplanted to the greenhouse, and a hole of 10 × 10 cm was punched in fertile loam covered with a black film with a hole puncher, and the Put the potato seedlings in the hole, cover with soil, and water. Cultivate and grow under the conditions of 24°C and 2500 lux, and complete acclimatization 7 days after transplanting.

[0027] When the seedlings grow a second new leaf, remove the tops to promote fork expansion. 45 days after transplanting, when the seedlings grow to 30cm, cut them into 1-leaf seedlings and cut them in the soil of the greenhouse at 30°C.

[0028] From the 35th day of the potato seedling growth period, hang the small peak card of Lily aphid at the base of the sweet potato leaves, and release it in 7 times, once every 10 days, 3000 bees / mu for the first time, and 2000 bees for the second time 3400 bees / mu, 3 to 7 times each r...

Embodiment 2

[0032] The virus-tested Jizishu No. 1 virus-free sweet potato test-tube seedlings obtained by conventional technical means were transplanted into the fertile sandy loam soil covered with black film in the greenhouse. Cultivate and grow at 25°C, 3000 lux, and complete acclimatization 7 days after transplanting.

[0033] When the seedlings grow a second new leaf, remove the tops to promote fork expansion. When the seedlings grow to 28cm 50 days after transplanting, cut them into 1-leaf seedlings and cut them in the soil of the greenhouse at 32°C.

[0034] From the 37th day of the potato seedling growth period, hang the small peak card of Lily aphid at the base of the sweet potato leaves, and release it in 7 times, once every 10 days, 2800 bees / mu for the first time, and 2200 bees for the second time / mu, 3-7 times each release 3000 bees / mu, a total of 2.0 × 10 bees 4 head / mu.

[0035] The above virus-free sweet potato seedlings were planted in the field on June 10 to obtai...

Embodiment 3

[0038] The Jishu 25 virus-free sweet potato test-tube seedlings obtained by conventional technical means and tested for viruses were transplanted to the greenhouse. Use a hole puncher to make a hole of 10×10 cm in the fertile loam covered with black film, put the potato seedlings with the medium into the hole, cover it with soil, and then water it. Cultivate and grow under the conditions of 23°C and 2000 lux, and complete the acclimatization after 7 days of transplanting.

[0039] When the seedlings grow a second new leaf, remove the tops to promote fork expansion. 48 days after transplanting, when the seedlings grow to 30cm, cut them into 1-leaf seedlings and cut them in the soil of the greenhouse at 28°C.

[0040] From the 35th day of the potato seedling growth period, hang the small peak card of Lily aphid at the base of the sweet potato leaves, and release it in 7 times, once every 10 days, 2500 bees / mu for the first time, and 2500 bees for the second time / mu, 3-7 time...

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Abstract

The invention provides a propagation method of sweet potato seedlings, which belongs to the technical field of rapid propagation. The method comprises the following steps: (1) transplanting the sweetpotato detoxification test-tube seedlings into a greenhouse, culturing the sweet potato detoxification test-tube seedlings for one to seven days, and obtaining a rapid propagation parent plant; (2) when a second new leaf grows on the parent plant, removing the top, performing the rapid propagation culture for 40 to 50 days, and obtaining the rapid propagation seedlings; (3) cutting the rapid propagation seedlings obtained in step (2) into primary rapid propagation seedlings, wherein each primary rapid propagation seedling is a rapid propagation seedling stem comprising one leaf; and (4) performing the cutting culture for the primary rapid propagation seedlings obtained in step (3), and obtaining the propagated sweet potato seedlings. By adopting the propagation method of the invention, thesurvival rate of the propagated sweet potato seedlings is 95.7 to 97.9 percent, the propagation multiplication is 660 to 750, and the virus carrier rate is reduced by 63 to 71 percent.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation, and in particular relates to a method for green rapid propagation of sweet potato virus-free test-tube seedlings in a greenhouse. Background technique [0002] Sweet potato is an important food crop in my country. In recent years, sweet potato virus disease has seriously endangered the production of sweet potato, which is one of the main reasons leading to the reduction of yield, quality and seed quality of sweet potato. Sweet potato is a vegetatively propagated crop. Due to long-term vegetative reproduction, the virus can be transmitted through tubers and seedlings, and the accumulation of the virus increases accordingly, resulting in a serious decrease in the yield and variety of sweet potato. At present, there is no effective chemical control method for sweet potato virus, and no sweet potato virus-resistant varieties have been bred in my country. Therefore, the virus-free breeding...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 侯夫云王庆美李爱贤董顺旭秦桢解备涛段文学张海燕张立明
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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