Microbial seedling culture medium and preparation method and application thereof

A technology for raising seedling substrates and microorganisms, which is applied in the field of agricultural production, can solve the problems of poor air permeability of the substrates, complicated formulas, and hardening, and achieves the effect of improving the germination rate and the growth amount.

Inactive Publication Date: 2018-11-27
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] High-quality seedlings are the prerequisite for high-yield vegetables, and the substrate is the material basis for high-quality seedlings. In production, vegetable seedlings often grow slowly, or grow weakly, or die in pieces, or the formula is cumbersome, or the management is complicated. And other problems, in response to these problems, we conducted a formula screening, focusing on several key points: 1. First, the substrate must hav

Method used

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  • Microbial seedling culture medium and preparation method and application thereof
  • Microbial seedling culture medium and preparation method and application thereof
  • Microbial seedling culture medium and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 7 recipe combinations:

[0035]

[0036] (+ stands for containing the ingredient, - stands for not containing the ingredient)

[0037] The preparation method of substrate microorganism seedling cultivation substrate:

[0038] (1) Mix peat soil, vermiculite, perlite, decomposed chicken manure, and diammonium according to the above proportions;

[0039] (2) Fermentation culture of Bacillus subtilis B579, slope-triangular flask-100 liters fermentation tank; the detailed process of fermentation and cultivation of Bacillus subtilis B579 is as follows:

[0040] (3) Centrifuge and concentrate Bacillus subtilis B579 at a speed of -2000r / min-. Reach 109cfu / ml;

[0041] (4) Spray 500 ml of the concentrated bacterial solution evenly into (1), stir evenly, and pack into bags;

[0042] The Bacillus subtilis B579 fermentation medium is beef extract peptone solid medium (slope) and liquid medium (fermentation tank). The medium components are: beef extract 5.0g, protein jelly 10...

Embodiment 2

[0045] Table 1 Conductivity and pH of substrates with different formulations

[0046]

[0047] By reviewing the literature, we preliminarily determined that formulas 3, 4, 5, 6, and 7 are closer to the standard values ​​and are more suitable for the growth of vegetable seedlings.

Embodiment 3

[0049] According to the combination of 7 formulas, four vegetable crops (tomato, cucumber, pepper, eggplant) were selected to test the influence on the germination rate of the above four crops, and 32-hole seedling trays were selected, each tray was filled with 3 liters of substrate, watered 500 ml, 4 kinds of crops were sown at the same time, one seed was sown in each hole of cucumber, two seeds were sown in each hole of tomato, pepper, and eggplant, and each treatment was repeated 5 times. .

[0050] Table 2 different seedling-raising matrix formulations are used for the impact of 3, the microbial seedling-raising matrix described in claim 1

[0051]

[0052] Through the above data results, we determine formulas 3, 4, 5, and 6 from the formula cost and the effect on vegetable crop seed germination.

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Abstract

The invention discloses a microbial seedling culture medium and a preparation method and application thereof. The microbial seedling culture medium mainly comprises peat soil, vermiculite, perlite, thoroughly decomposed chicken manure, diamonium and the like. The particle size of the microbial seedling culture medium is 1-10mm, the pH of the microbial seedling culture medium is 6.8-7.3, the conductivity of the microbial seedling culture medium is 51.5-60.4ms/cm, and the spore content of the microbial seedling culture medium is 105/g. The microbial seedling culture medium has the advantages that the microbial seedling culture medium can provide all nutrition need by the growth of vegetable seedlings, nutrient solution irrigation is not needed any more, and diseases such as damping off and blight of the vegetable seedlings can be effectively prevented and treated. The microbial seedling culture medium is applicable to the growth of the vegetable seedlings and the growth of pot-cultured flowers.

Description

technical field [0001] The invention belongs to the technical field of agricultural production, and specifically relates to a seedling-raising substrate which has nutrition, air permeability, water retention and disease prevention properties, and is suitable for industrial vegetable seedling raising and flower growth period use. Background technique [0002] High-quality seedlings are the prerequisite for high-yield vegetables, and the substrate is the material basis for high-quality seedlings. In production, vegetable seedlings often grow slowly, or grow weakly, or die in pieces, or the formula is cumbersome, or the management is complicated. And other problems, in response to these problems, we conducted a formula screening, focusing on several key points: 1. First, the substrate must have air permeability, that is, the requirement for the porosity of the substrate. Poor air permeability of the substrate will cause aerobic microbial activity to decrease. Strong water absor...

Claims

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Application Information

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IPC IPC(8): A01G24/28A01G24/20A01G24/15A01G24/10C05G3/00
CPCA01G24/10A01G24/15A01G24/20A01G24/28C05B7/00C05G3/00C05D9/00C05F3/00C05F11/02C05F11/08
Inventor 杨秀荣孙淑琴孙冰冰田涛刘亦学张惟
Owner 天津市农业科学院
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