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Walnut leaf endophytic biocontrol bacterium as well as preparation method and application thereof

A technology of leaf and walnut leaf, which is applied in the field of endogenous biocontrol bacteria in walnut leaves and its preparation, to achieve low production and use costs, high safety, and good control effect

Pending Publication Date: 2022-01-07
HEBEI AGRICULTURAL UNIV.
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the control of these two diseases mostly focuses on chemical control. The inventors found that Bacillus subtilis Bacillus subtilis It has a significant inhibitory effect on the spore germination and mycelium growth of Fusarium laminarum and Glososporum anthracnose, but the antifungal activity of this fungus has not been reported in walnut leaf diseases

Method used

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  • Walnut leaf endophytic biocontrol bacterium as well as preparation method and application thereof
  • Walnut leaf endophytic biocontrol bacterium as well as preparation method and application thereof
  • Walnut leaf endophytic biocontrol bacterium as well as preparation method and application thereof

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Embodiment Construction

[0028] The present invention will be described in detail below in conjunction with examples, which are only preferred implementations of the present invention, and are not limitations of the present invention.

[0029] In a specific embodiment, Bacillus subtilis Bacillus subtilis The (TL3) strain has been deposited in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on June 15, 2021; the address of the preservation unit: Beijing, China. The Institute of Microbiology, Chinese Academy of Sciences; the preservation number is CGNCC No: 22705.

[0030] Bacillus subtilis Bacillus subtilis (TL3), which can effectively control walnut anthracnose or walnut leaf blight caused by Fusarium laminarum.

[0031] The preparation method of TL3 bacterial strain comprises the following steps:

[0032] S1. Wash the walnut leaves to remove impurities and then perform surface disinfection. After disinfection, grind them into a homogenate in a st...

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Abstract

The invention belongs to the technical field of microorganisms. The invention discloses a walnut leaf endophytic biocontrol bacterium, which belongs to bacillus, the preservation number of the walnut leaf endophytic biocontrol bacterium is CGNCC No: 22705, and the 16S rDNA nucleotide sequence of the walnut leaf endophytic biocontrol bacterium is shown as SEQ ID NO.1. The walnut leaf endophytic biocontrol bacterium is high in safety, capable of effectively preventing and treating walnut leaf blight caused by walnut anthracnose or fusarium laminar, the walnut leaf endophytic biocontrol bacterium has low production and use cost, is free of residues, is high in safety, is environmentally friendly and is good in prevention and treatment effect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an endogenous biocontrol bacterium in walnut leaves and a preparation method and application thereof. Background technique [0002] Walnut (Juglans regia) is an important woody oil crop in my country. Anthracnose caused by glenospora anthracnose and leaf blight caused by fusarium laminarum will cause a large area of ​​leaf death, seriously affecting the photosynthesis of the tree and the accumulation of nutrients in the tree. At present, the control of these two diseases mostly focuses on chemical control. The inventors found that Bacillus subtilis Bacillus subtilis It has a significant inhibitory effect on the spore germination and hyphal growth of Fusarium laminarum and G. anthracnose, but the antifungal activity of this fungus has not been reported in walnut leaf diseases. Contents of the invention [0003] The technical problem to be solved by the present inv...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/22Y02A50/30
Inventor 阎爱华王子夜
Owner HEBEI AGRICULTURAL UNIV.
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