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Application of rhizoma paridis extract in preparation of drugs for treating human prostate cancer

A technique for aesculus and prostate cancer, which can be used in drug combinations, antitumor drugs, pharmaceutical formulations, etc., and can solve problems such as unreported

Inactive Publication Date: 2018-11-23
HUBEI UNIVERSITY OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of PPI on autophagy and the therapeutic effect of combination drugs on human prostate cancer have not been reported so far.

Method used

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  • Application of rhizoma paridis extract in preparation of drugs for treating human prostate cancer
  • Application of rhizoma paridis extract in preparation of drugs for treating human prostate cancer
  • Application of rhizoma paridis extract in preparation of drugs for treating human prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, papaya saponin I inhibits the growth of prostate cancer cells

[0024] Prostate cancer DU145 and PC3 cell lines in the logarithmic growth phase were inoculated in 96-well plates (approximately 5000 cells per well, 90 μl medium), cultured (37 ° C, 5% CO2 incubator) for 24 hours and then used different Two kinds of cells were treated with polyphyllin I (PPI) with concentration gradient (0.1-3μmol / L). After culturing for 20 and 44 hours, add 10 μl of 5 mg / ml 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) solution to each well, continue Incubate for 4 hours. After the reaction was terminated, the culture medium was sucked off, 150 μl dimethyl sulfoxide was added to each well, shaken at a low speed to fully dissolve, and then the absorbance at 490 nm (OD490) was measured with a microplate reader to calculate the survival rate.

[0025] For experimental results, see figure 1 A and figure 1 b. The results show that different concentrations of ...

Embodiment 2

[0026] Example 2, papaya saponin I can induce the increase of autophagosomes

[0027] The LC3 overexpression vector was constructed, and the DsRed gene was added to the vector to express the red fluorescent protein. When the transfected vector entered the cells, the red fluorescent protein would also show red fluorescence under 554nm excitation light while the LC3 protein carried by the vector was expressed. Since the LC3I subtype is a water-soluble protein, it exists in a diffuse state in cells; while the LC3II subtype is a water-insoluble protein, which exists in an aggregated state in water. Therefore, by observing the distribution of red fluorescence in cells by immunofluorescence, we can understand the distribution of LC3I subtypes and LC3II subtypes in cells, and further determine whether autophagy occurs in cells.

[0028] 1. Plate the prostate cancer cell lines DU145 and PC3 at a density of 5×105 cells / mL in a 12-well plate covered with glass slides (four wells). After...

Embodiment 3

[0034] Example 3. Paprika saponin I can promote the increased expression of human prostate cancer cell LC3BII

[0035] LC3 is a landmark protein in the autophagy signaling pathway, including two subtypes, LC3I and LC3II. When cells do not undergo autophagy, the protein exists in the cytoplasm as a water-soluble LC3I subtype; when cells undergo autophagy, the protein converts into a water-insoluble LC3II subtype. By detecting the amount of LC3II in the cells, the strength of autophagy can be determined.

[0036] 1. Two PPI-treated cancer cells:

[0037] ① Divide DU145 cells into four groups, put them into DMEM medium containing 10% FBS with PPI concentrations of 0 μmol / L, 0.6 μmol / L, 0.7 μmol / L and 0.8 μmol / L respectively, and culture them at 37°C for 24 hours , respectively collected DU145 cells treated with 0 μmol / L PPI, DU145 cells treated with 0.6 μmol / L PPI, DU145 cells treated with 0.7 μmol / L PPI, and DU145 cells treated with 0.8 μmol / L PPI.

[0038]② Divide the PC3 ce...

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Abstract

The invention discloses application of a rhizoma paridis extract in preparation of drugs for treating human prostate cancer. According to the application, experiments shows that polyphyllin I has an inhibition effect on the human prostate cancer DU145 and PC3 cell lines, and the inhibition effect is improved along with the increase of dosage and time; in addition, the polyphyllin I can induce theincrease of autophagosomes and promote the expression increase of human prostate cancer cells LC3B-II, and therefore the growth of xenografted tumors of DU145 cells can be inhibited; and the polyphyllin I is a potential, novel and effective anti-prostate-cancer drug and can be used for preparing drugs for treating the human prostate cancer, a new way for treating the human prostate cancer is opened up, and the medical application range of the polyphyllin I is widened.

Description

technical field [0001] The present invention relates to a new application of the extract of Aescinus aescini in the field of medicine, more specifically, it relates to the application of papaya saponin I in the preparation of medicine for treating human prostate cancer. Background technique [0002] Prostate cancer is one of the most common malignancies in men. In 2012, there were approximately 1,111,700 new cases worldwide, and the death toll has reached 307,500. The incidence of prostate cancer in China has increased significantly in recent years. Although survival rates of >5 years have been reported for patients with localized prostate cancer, most patients with prostate cancer eventually experience castration resistance, leading to recurrence. Therefore, it is imminent to find new drugs that can inhibit the tumorigenesis, development and metastasis of prostate cancer cells. [0003] Autophagy, also known as type II programmed cell death, refers to the process in w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00A61P13/08
CPCA61K31/7048A61P13/08A61P35/00
Inventor 刘莹司渊张特刘鹏飞刘雪文许佳欣
Owner HUBEI UNIVERSITY OF MEDICINE
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