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Preparation method of marine oligopeptide with uric acid reducing activity

An oligopeptide and uric acid-lowering technology, which can be applied to medical preparations containing active ingredients, pharmaceutical formulas, peptide/protein components, etc. High yield, reducing the effect of toxic and side effects

Inactive Publication Date: 2018-11-20
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Skipjack is a low-fat and high-protein seafood with rich nutritional value, but there is no report on the optimization of the preparation process of skipjack oligopeptides with uric acid-lowering activity and its biological activity.

Method used

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  • Preparation method of marine oligopeptide with uric acid reducing activity
  • Preparation method of marine oligopeptide with uric acid reducing activity
  • Preparation method of marine oligopeptide with uric acid reducing activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Pretreatment of bonito raw materials: Wash bonito → remove viscera, fish head, fish tail → collect meat → grind and mix well with high-speed tissue homogenizer → freeze for later use.

[0026] 2. Calculation of the yield of bonito oligopeptides:

[0027]

[0028] Among them, the acid-soluble protein hydrolyzate content: refer to the method of GB / T 22729-2008 for determination. Take 5 mL of skipjack protein enzymatic hydrolyzate, add 5 mL of 15% trichloroacetic acid, mix well, let stand for 10 min, centrifuge at 8000 r / min, 4°C for 10 min, and take the supernatant as the test solution. Weigh 3 g of the solution to be tested and put it in a digestion tube, remove water in a digestion furnace, add 1 piece of FOSS tablet and 15 mL of concentrated sulfuric acid after cooling, digest, cool, and use Kjeltec8400 automatic azotometer to determine acid-soluble protein hydrolyzate The content is in mg N / g.

[0029] Amino acid nitrogen content: determined according to GB500...

Embodiment 2

[0067] After 7 days of adaptive feeding, 96 ICR mice were randomly divided into six groups, 16 mice in each group, which were normal group, model group, positive control group (allopurinol group), and low-dose skipjack fish hydrolyzate group (200 mg / kg), middle-dose group (600 mg / kg), high-dose group (1000 mg / kg), each group was administered as shown in Table 5 for 7 consecutive days, and fasted on the sixth day of administration Overnight, on the seventh day, one hour after the intraperitoneal injection, the blood was collected from the eyeball. After the blood was allowed to stand for half an hour, it was centrifuged at 3000 rpm / min at 4°C for 10 minutes. The supernatant was serum, and stored at 4°C for testing. The liver was quickly taken out on the ice platform, put into liquid nitrogen for quick freezing, and then transferred to -80°C for storage for later use.

[0068] Table 5 Grouping and administration of animals

[0069]

[0070] 1. Effect of samples on body weigh...

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Abstract

The invention discloses a preparation method of marine oligopeptide with uric acid reducing activity, and relates to the technical field of deep processing of aquatic products. The preparation methodof the oligopeptide comprises the following steps: processed bonito surimi is subjected to enzymolysis by 4400 U / g of flavourzyme for 6.5 h at the temperature of 42 DEG C, freeze-drying is performed after enzymolysis, and the bonito oligopeptide is prepared. The prepared bonito oligopeptide can be used for antagonizing hyperuricemia in mice, reducing toxic and side effects of drug treatment and treatment cost, is good for reducing incidence of chronic diseases such as gout, and has good market prospect.

Description

technical field [0001] The invention belongs to the technical field of deep processing of aquatic products, and in particular relates to a preparation method of bonito oligopeptide which has a good antagonistic effect on mouse hyperuricemia. Background technique [0002] Skipjack, commonly known as bomb fish, belongs to the class of bony fishes, scombroids, and perciformes. Skipjack is extremely rich in reserves, and the fish is red meat, delicious and smooth. In addition, the nutritional value of its fish diet is extremely high. Not only is it rich in protein, but also the types of amino acids are relatively diverse and in reasonable proportions. [0003] Hyperuricemia is the detection of blood uric acid levels twice on an empty stomach on different days under the normal purine diet. It is the basis of gout, and it is also a factor leading to hypertension, hyperlipidemia, coronary heart disease, metabolic syndrome, kidney damage and other diseases. . As a disease with hi...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P19/06
CPCA61K38/012A61P19/06C12P21/06
Inventor 孔祥佳刘智禹李守江谢勇林埔吴仲
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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