Chronic thromboembolic pulmonary hypertension rat model and building method thereof

Abstract

The invention belongs to the technical field of a new variety of vertebrates, and discloses a chronic thromboembolic pulmonary hypertension rat model and a building method thereof. Chloral hydrate ata concentration of 10 percent is injected through the abdominal cavity for anesthesia; under a sterile condition, through a left internal jugular vein, 1.5 mL of tranexamic acid-containing normal saline mixed with about 32 + / - 5 autologous thromboemboli embolizes the pulmonary artery at a rate of 0.5 ml / min; the rat is slowly turned in the embolizing process to enable a larger number of emboli toenter the lung circulation of the rat; after being embolized, the rat has cyanosis and breathes faster; on the fourth day and the eleventh day of the embolization, the rat is embolized for the secondtime and the third time according to the same method; the rat is observed for 5 weeks. The CTEPH model built by the building method disclosed by the invention takes the autologous emboli of the rat asemboli to embolize the pulmonary artery and enables the autologous emboli to embolize a blood vessel as long as possible under the help of pro-inflammatory effect of carrageenin, so as to really model the whole pathophysiological process of pulmonary embolism secondary CTEPH of a clinical CTEPH patient.

Description

technical field [0001] The invention belongs to the technical field of new varieties of vertebrates, and in particular relates to a chronic thromboembolic pulmonary hypertension rat model and a construction method thereof. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Chronic thromboembolic pulmonary hypertension (CTEPH) is acute pulmonary embolism (APE) or pulmonary artery thrombosis in situ, the thrombus persists due to various reasons without dissolution, through organization, fibrosis, severe Consequences of pulmonary vascular obstruction. Although the incidence of CTEPH is low, the prognosis is very poor. Continuously elevated pulmonary vascular resistance will eventually lead to the development of right heart failure and death. Research data show that the level of pulmonary artery pressure is closely related to the prognosis. Currently, pulmonary artery thromboendarterectomy (Pulmonary End...

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Problems solved by technology

[0005] (1) Animals have high fibrinolytic ability and no spontaneous lower extremity deep vein thrombosis, and it is difficult to establish an ideal animal model for CTEPH

[0006] (2) There have been models of embolic pulmonary hypertension of different animal species such as pigs, dogs, and rats, but the embolic substances are non-thrombotic substances such as small glass beads, polystyrene microspheres, and polidocanol, which cannot be truly simulated The pathophysiological process similar to clinical CTEPH patients, such as the stimulation of blood vessels by pulmonary thrombus

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