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A method for detecting ancient woolen fabric fragments based on Western blotting

An immunoblotting method and wool fabric technology, applied in the field of cultural relics detection, can solve the problems of wool cultural relics identification and protection research difficulties, the identification results are not fully accurate, and the analysis of fiber morphology is not enough, and the size can be adjusted and accurately The effect of high precision and high sensitivity

Active Publication Date: 2021-02-02
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After being unearthed, due to drastic changes in environmental conditions, the aging of fibers was further accelerated. Therefore, it is not difficult to imagine that in the long history, woolen fabrics buried in tombs or ruins have already lost their original appearance and degraded into Peptides or amino acids cannot be recognized by the naked eye, and the older the evidence, the harder it is to find, which brings great difficulties to the identification and protection of wool cultural relics
[0004] At present, ordinary analysis and detection techniques, such as Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction analysis, etc., are often at a loss when encountering such residue analysis. Most of the identification of ancient woolen fabrics at home and abroad still remain In the analysis of fiber morphology, the identification results obtained are not completely accurate; therefore, it is very necessary to find a more scientific method to identify ancient woolen fabrics

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1) Preparation of carbon quantum dots: Add water and sodium dodecylbenzenesulfonate at a molar mass ratio of 20:1 to 20 mL of organic solvent n-pentane to prepare an oil phase solution; add to the oil phase solution, Add an aqueous solution of biomass tar with a mass fraction of 50%, then add n-propanol and sodium dodecylbenzenesulfonate with a molar mass ratio of 2:1, shake vigorously to obtain an inverse microemulsion, and let it stand for 12 hours; Add 0.3 g of dodecylamine to the microemulsion, ultrasonicate for 10 min at room temperature, pour it into a reaction kettle containing a polytetrafluoroethylene liner, place it in a muffle furnace at 120 °C for 3 h, take it out, and cool it down to room temperature naturally. Demulsify with anhydrous methanol, wash several times, and centrifuge to obtain a carbon quantum dot solution. Half of the solution was dialyzed with a dialysis bag with a molecular weight cut off of 1000 for 24 h, and then freeze-dried to obtain car...

Embodiment 2

[0066] 1) Preparation of carbon quantum dots: Add water and sodium dodecylbenzenesulfonate at a molar mass ratio of 18:1 to 15 mL of organic solvent n-pentane to prepare an oil phase solution; add to the oil phase solution, Add an aqueous solution of biomass tar with a mass fraction of 45%, then add n-propanol and sodium dodecylbenzenesulfonate with a molar mass ratio of 1.8:1, and shake vigorously to obtain an inverse microemulsion, which is left to stand for 10 h; Add 0.2 g of dodecylamine to the microemulsion, ultrasonicate it for 8 min at room temperature, pour it into a reaction kettle containing a polytetrafluoroethylene liner, place it in a muffle furnace at 110 °C for 2 h, take it out, and cool it down to room temperature naturally. Demulsify with anhydrous methanol, wash several times, and centrifuge to obtain a carbon quantum dot solution. Half of the solution was dialyzed for 20 h with a dialysis bag with a molecular weight cut off of 1000, and freeze-dried to obtai...

Embodiment 3

[0080] 1) Preparation of carbon quantum dots: Add water and sodium dodecylbenzenesulfonate at a molar mass ratio of 22:1 to 25 mL of organic solvent n-pentane to prepare an oil phase solution; add to the oil phase solution, Add an aqueous solution of biomass tar with a mass fraction of 55%, then add n-propanol and sodium dodecylbenzenesulfonate with a molar mass ratio of 2.2:1, shake vigorously to obtain a reverse-phase microemulsion, and let it stand for 13 hours; Add 0.4 g of dodecylamine to the microemulsion, sonicate for 12 min at room temperature, pour it into a reaction kettle containing a polytetrafluoroethylene liner, place it in a muffle furnace at 130 °C for 4 h, take it out, and cool it down to room temperature naturally. Demulsify with anhydrous methanol, wash several times, and centrifuge to obtain a carbon quantum dot solution. Half of the solution was dialyzed for 28 hours with a dialysis bag with a molecular weight cut off of 1000, and freeze-dried to obtain ca...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Abstract

The invention relates to the field of detection of cultural relics, and discloses a method for detecting ancient wool fabric fragments based on immunoblotting. The invention firstly prepares a wool secondary antibody labeled with carbon quantum dots, and then uses metal salt method and reduction method to extract modern wool and ancient wool fabrics The fragments were subjected to SDS-PAGE gel electrophoresis, stained with carbon quantum dot solution, and the electrophoresis image was observed. The obtained protein bands were transferred to polyvinylidene fluoride membrane, and after incubation with the wool primary antibody and the carbon quantum dot-labeled secondary antibody, the immune bands could be observed in the gel imaging system to identify ancient wool fabrics. The invention has the advantages of less consumption of chemical reagents, mild reaction, environmental protection and harmlessness; when detecting ancient wool fabrics, it has the advantages of less consumption of samples, sensitivity, accuracy, intuition and the like.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for detecting ancient wool fabric fragments based on the immunoblotting method. Background technique [0002] China has a long history. A large number of wool products have been unearthed from early Chinese tombs. The quantity and the exquisite weaving are both amazing. The study of these unearthed wool fabrics plays a decisive role in understanding the origin of ancient wool fabrics, ethnic minority costumes, and inter-ethnic cultural exchanges. [0003] Among the remains of ancient wool fabrics, wool remains are mainly wool fabric fragments. Such remains have been buried in the underground environment for a long time. Under the influence of environmental factors such as water, heat, oxygen, and microorganisms, wool keratin will undergo denaturation and aging degradation. After being unearthed, due to drastic changes in environmental conditions, the aging of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 王秉王钟元胡铭周周莲彭志勤胡智文
Owner ZHEJIANG SCI-TECH UNIV
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