Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kit for simultaneously detecting capecitabine and its metabolites in plasma, its detection method and application

A technology for capecitabine and its metabolites, which is applied in the field of kits for simultaneous detection of capecitabine and its metabolites in plasma, can solve the problems of large differences in physicochemical properties, different contents, and high technical difficulty, and achieve accurate The effect of high accuracy, high sensitivity, and simple pretreatment process

Active Publication Date: 2021-10-22
SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large differences in the physical and chemical properties of capecitabine and its metabolites, as well as the different levels in blood, the simultaneous detection technology is difficult. At present, there is no ultra-high performance liquid chromatography tandem mass spectrometry technology for the simultaneous detection of capecitabine in plasma. and its five metabolites reported in the literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kit for simultaneously detecting capecitabine and its metabolites in plasma, its detection method and application
  • A kit for simultaneously detecting capecitabine and its metabolites in plasma, its detection method and application
  • A kit for simultaneously detecting capecitabine and its metabolites in plasma, its detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Experimental materials

[0056] The plasma samples used came from the plasma of patients with colorectal cancer who took capecitabine in Shanghai Changzheng Hospital from October to November 2016.

[0057] (1) Instruments. Agilent 6460A triple quadrupole mass spectrometer; Agilent 1290Series ultra-high performance liquid chromatography; adjustable pipettes (Eppendorf, 0.5-10 μL, 10-100 μL, 100-1000 μL); one hundred thousandth balance (Sartorius company, CPA255D type); Vortex mixer (Labnet Company, VX-200 type); Centrifuge (Beili Company, DT-5 type); Freeze drying apparatus (Gene Company, Scanspeed Mari Vac Alpha type).

[0058] (2) Reagent consumables. Chromatographically pure methanol and analytically pure acetonitrile (Merck); chromatographically pure formic acid (Tiandi); double distilled water (Watsons); ethyl acetate (Caledon); isopropanol (Thermo Fisher).

[0059] (3) The reference substance of the compound to be tested and the internal standard reference su...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a kit for simultaneously detecting capecitabine and its metabolites in plasma. Capecitabine and its metabolites are respectively: capecitabine, 5'-deoxy-5-fluorocytidine, 5 '-deoxy-5-fluorouridine, 2'-deoxy-5-fluorouridine, 5-fluorouracil, 5-fluoro-5,6-dihydrouracil; the simultaneous detection of capecitabine and its The kit for metabolites includes the following reagents: eluent, reference substance mother solution, mixed internal standard solution, diluent, extraction solution, and quality control solution. The invention also discloses an application of the kit for simultaneously detecting capecitabine and its metabolites in blood plasma in the preparation of hematological diagnosis or screening kits for malignant tumors. The kit for simultaneously detecting capecitabine and its metabolites in blood plasma of the present invention is used to simultaneously detect capecitabine and its metabolites, which has high sensitivity, strong specificity, high accuracy and simple pretreatment process.

Description

technical field [0001] The invention belongs to the technical field of blood quantitative detection, and specifically relates to a kit for simultaneously detecting capecitabine and its metabolites in blood plasma, a detection method and an application thereof. Background technique [0002] Since the early 1990s, cancer has become the number one cause of death in my country, among which malignant tumors of the digestive tract occupy the forefront of the morbidity and mortality of all malignant tumors, seriously endangering people's health and life [item] Yong Bing. The prevalence and current situation of malignant tumors of the digestive tract. Tumors, 2002,22(4):336-339.]. According to statistics, in 2013, there were about 1.05 million new patients with digestive tract malignant tumors nationwide, accounting for about 30% of the total incidence of malignant tumors, including about 420,000 new gastric cancer patients, 350,000 new colorectal cancer patients, 280,000 patients; ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/045
Inventor 王志鹏高守红杨阳陈万生恽芸蕾张凤李明明仲人前
Owner SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com