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Method for detecting tanshinol and its metabolites in biological sample

A technology of biological samples and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2018-11-13
北京鸿测科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The existing detection methods of danshensu metabolites can detect very few danshensu metabolites, and this information is far from enough to explain the metabolic mechanism of danshensu in vivo

Method used

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  • Method for detecting tanshinol and its metabolites in biological sample
  • Method for detecting tanshinol and its metabolites in biological sample
  • Method for detecting tanshinol and its metabolites in biological sample

Examples

Experimental program
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Effect test

Embodiment 1

[0056] 1 Experimental instruments and materials

[0057] DIONEX Ultimate 3000 ultra-high performance liquid chromatography analysis system: American ThermoFisher Company;

[0058] LTQ-Orbitrap XL mass spectrometer: American Thermo Fisher Company, equipped with electrospray ion source (ESI) and Xcalibur 2.1 workstation;

[0059] Grace Pure TM SPEC 18 -Low SPE cartridge (500mg / 3mL);

[0060] Milli-Q Synthesis ultrapure water purification system: Millipore, USA;

[0061] R200D electronic analytical balance (1 / 100,000): German Sartorius company;

[0062] KQ-250DE CNC ultrasonic cleaner: Kunshan Ultrasonic Instrument Co., Ltd.

[0063] Danshensu reference substance: product of Chengdu Master Biological Technology Co., Ltd. (purity greater than 98%);

[0064] Formic acid (chromatographically pure): Germany Merck company;

[0065] Methanol (mass spec pure) and acetonitrile (mass spec pure): American Thermo Fisher Company.

[0066] Sprague Dawley (SD) rats (male, weighing 220...

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Abstract

The invention discloses a method for detecting tanshinol and its metabolites in a biological sample. The method is characterized by comprising the following steps that the biological sample is treated; a solution to be detected is detected by adopting an ultra-high performance liquid chromatography-mass spectrometer. By adopting the detecting method, more tanshinol metabolites in the biological sample can be detected, and accordingly a foundation is laid for illumination of tanshinol metabolism pathways.

Description

technical field [0001] The invention relates to a detection method for danshensu and its metabolites in biological samples. Background technique [0002] Salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge., a plant in the Labiatae family. It is a traditional medicine for promoting blood circulation and removing stasis in my country. Modern research shows that the main chemical components of Danshen are fat-soluble diterpene quinones and water-soluble phenolic acids. Danshensu, as an index component of Danshen, has various pharmacological effects such as anti-inflammation, inhibition of thrombosis, neuroprotection, anti-tumor, improvement of heart function, and expansion of coronary arteries. It is often used as a quality control index of Danshen medicinal materials or Danshen compound preparations. However, the in vivo metabolic process and metabolites of danshensu are still unclear, and further research is urgently needed. [0003] "Study on the Ph...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/06
CPCG01N30/02G01N30/06G01N30/72G01N2030/062
Inventor 王志斌张加余张连中
Owner 北京鸿测科技发展有限公司
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