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Method for evaluating influence of to-be-tested objects on exercise tolerance of organic body and special equipment of method

A technology for sports endurance and objects to be tested, applied in the field of biological experiments, can solve problems affecting the accuracy of experimental data, unfavorable data stability control, unstable water flow, etc., to ensure accuracy and reproducibility, good promotion prospects, The effect of short experimental period

Pending Publication Date: 2018-11-13
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole set of equipment provided by Loligo is bulky, and the overall price of its hardware is about 220,000 RMB, which is relatively expensive, and the water flow in open water bodies is not stable, which is not conducive to the stable control of experimental data and affects the accuracy of experimental data. sex
[0005] In addition, after searching the Internet through different channels, it was found that the patents of the Yangtze River Fisheries Research Institute of the Chinese Academy of Fishery Sciences (CN103202254B) and the China Three Gorges University (CN204047610U) were designed to measure the swimming ability of large fish. The volume is too large and can be manufactured. Maximum flow rate is too low

Method used

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  • Method for evaluating influence of to-be-tested objects on exercise tolerance of organic body and special equipment of method
  • Method for evaluating influence of to-be-tested objects on exercise tolerance of organic body and special equipment of method
  • Method for evaluating influence of to-be-tested objects on exercise tolerance of organic body and special equipment of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Test of the influence of 0.1% ethanol solution on the endurance of zebrafish

[0065] 1. Correct the water flow rate of the system: turn on the equipment, adjust the water flow control valve and start the water pump to generate water flow, record and test the flow value corresponding to the frequency of the frequency conversion motor of the water pump, and observe the adjustment and correction through the flow control valve and flow meter.

[0066] 2. Screening experimental zebrafish experimental fish: before the experiment, put the zebrafish fish into a hollow transparent round tube filled with water, maintain an anhydrous flow rate, let the zebrafish adapt for 3 minutes, and gradually adjust the water flow rate to 6.8BL / sec , adapt for 1 minute, gradually adjust the water flow rate to 7.6BL / sec for 10 minutes, and screen zebrafish experimental fish that can complete this step.

[0067] 3. Swimming endurance test: Prepare a 0.1% ethanol solution, design a bl...

Embodiment 2

[0070] Example 2: Effects of creatine and β-alanine solutions on zebrafish endurance

[0071] 1. Correct the water flow rate of the system: turn on the equipment, adjust the water flow control valve and start the water pump to generate water flow, record and test the flow value corresponding to the frequency of the frequency conversion motor of the water pump, and observe the adjustment and correction through the flow control valve and flow meter.

[0072] 2. Screening experimental zebrafish experimental fish: before the experiment, put the zebrafish into a hollow transparent tube filled with water, let the zebrafish adapt for 3 minutes, gradually adjust the water flow rate to 6.8BL / sec, and adapt for 3 minutes. Gradually adjust the water flow rate to 7.6BL / sec for 10 minutes, and screen zebrafish experimental fish that can complete this step.

[0073] 3. Swimming endurance test: design a blank control group, an experimental group containing 500 μM creatine in the test contain...

Embodiment 3

[0076] Example 3: Comparison of Zebrafish and Catfish Maximum Swimming Endurance

[0077] 1. Correct the water flow rate of the system: turn on the equipment, adjust the water flow control valve and start the water pump to generate water flow, record and test the flow value corresponding to the frequency of the frequency conversion motor of the water pump, and observe the adjustment and correction through the flow control valve and flow meter.

[0078] 2. Screening experimental zebrafish experimental fish:

[0079] Before the experiment, put the zebrafish school into a hollow transparent tube filled with water, maintain an anhydrous flow rate, let the zebrafish adapt for 1 minute, gradually adjust the water flow rate to 6.8BL / sec, adapt for 1 minute, and gradually adjust the water flow rate to 7.6BL / sec was maintained for 10 minutes, and the zebrafish experimental fish that could complete this step were screened.

[0080]Before the experiment, put catfish fish into a hollow t...

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Abstract

The invention discloses a method for evaluating the influence of to-be-tested objects (an anti-fatigue drug, an anti-fatigue and a pollutant) on the exercise tolerance of an organic body and special equipment of the method. The method comprises the following steps: (1) correcting the flow speed of a system water body; (2) screening an experimental fish shoal; (3) testing the swimming resistance; and (4) collecting experimental data, and analyzing a result. The special equipment comprises a water tank, a water pump, a container for swimming of fishes, a flow regulation valve, a flow meter, a nitrogen bottle, an oxygen bottle, a refrigerator and a high-speed camera. The method can be used for carrying out pre-warning judgment on an abnormal environmental water body, testing a drug, evaluating the exercise tolerance of different species of small-sized fishes in different water body environments, evaluating and testing the a nutritious supplementary and the like. The equipment can be automatically assembled and occupies a small area, parameters such as the temperature, the water flow speed and the dissolved oxygen amount can be controlled, and the equipment is easy and convenient to operate, relatively low in manufacturing cost and convenient for the application of relevant biological experiments.

Description

technical field [0001] The invention relates to a method and special equipment for evaluating the effect of test objects (anti-fatigue drugs, anti-fatigue nutrients, pollutants) on the body's exercise endurance, and mainly relates to the field of biological experiments. Background technique [0002] Exercise endurance refers to the ability of the body to withstand the maximum exercise load, involving physiological load, exercise stress tolerance, etc., and is an important indicator reflecting the health status of the body. Appropriate experimental methods are used for evaluation, which is a powerful experimental tool for studying exogenous factors (such as environmental poisons, drugs, nutrition, etc.) on the body's exercise tolerance. Fish exercise endurance generally refers to the maximum continuous swimming ability of fish. The common indicators to measure the maximum continuous swimming ability of fish are the maximum continuous swimming speed and continuous swimming tim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K63/00A01K63/04A01K29/00
CPCA01K29/005A01K61/10A01K63/003A01K63/04A01K63/042Y02A40/81
Inventor 韩利文萧崇徳刘可春孔浩天李晓彬张云靳梦
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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