Eurotium cristatum-fermented functional compound, and preparation method and application thereof
A coronoid saccharomyces and compound technology, which is applied in the fields of application, function of food ingredients, food science, etc., can solve the problems of simple and single ingredients of the medium, insufficient efficacy of health care products, etc., and achieve fragrant taste and diverse nutritional ingredients Effect
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Embodiment 1
[0034] Such as figure 1 Shown is a method for preparing a fermentation functional compound of Saccharomyces coronarii, comprising the steps of:
[0035] Step 1: Prepare tea extract
[0036] Weigh 30kg of water, add 1.8kg of black tea dry tea, add active additives, the weight ratio of water: tea leaves: active additives is 100: 4-8: 0.5-3, soak at a constant temperature of 60-120 ° C Extract for 20-60 minutes, filter to remove tea dregs to obtain tea extract;
[0037] Step 2: Preparation of Fermentation Medium
[0038] Mix the tea extract prepared in Step 1 with the carbon source nutrients in a volume-to-mass ratio of 100:4-8, and prepare the pH of the prepared solution at 5.0-5.5, and then set the temperature at 115-121°C , the pressure is under the condition of 0.1-0.15MPa, and the fermentation medium is obtained by continuous sterilization for 20-30min;
[0039] Step 3: Prepare the suspension of S. coronoidis
[0040] Spread the strains of P. coronoidis on the PDA agar ...
Embodiment 2
[0050] like figure 1 Shown is a method for preparing a fermentation functional compound of Saccharomyces coronarii, comprising the steps of:
[0051] Step 1: Prepare tea extract
[0052] Weigh 30kg of water, add 2.0kg of dry black tea, then add 0.15kg of dried medlar, extract at a constant temperature of 120°C for 40min, filter to remove the tea dregs to obtain the tea extract;
[0053] Step 2: Preparation of Fermentation Medium
[0054] Mix the tea extract prepared in step 1 with glucose in a volume-to-mass ratio of 100:4 and prepare the prepared solution with a pH of 5.0-5.5. , and continue to sterilize for 20-30min to obtain the fermentation medium;
[0055] Step 3: Prepare the suspension of S. coronoidis
[0056] Smear the strains of P. coronis on the PDA agar medium, and cultivate them for 6 days at a constant temperature of 28°C. After adding 10ml of sterile water to the solid medium, the spores of P. coronis Scrape it off and place it in a container filled with ste...
Embodiment 3
[0063] like figure 1 Shown is a method for preparing a fermentation functional compound of Saccharomyces coronarii, comprising the steps of:
[0064] Step 1: Prepare tea extract
[0065] Weigh 30kg of water, add 2.4kg of dry black tea, 0.5kg of dried medlar, and 0.5kg of red dates, extract at a constant temperature of 100°C for 20 minutes, and filter to remove the tea dregs to obtain the tea extract;
[0066] Step 2: Preparation of Fermentation Medium
[0067] Mix the tea extract prepared in step 1 with glucose in a volume-to-mass ratio of 100:4 and prepare the pH of the prepared solution at 5.0. Sterilize for 30 minutes to obtain the fermentation broth;
[0068] Step 3: Prepare the suspension of S. coronoidis
[0069] Smear the strains of P. coronis on the PDA agar medium, and cultivate them for 4 days at a constant temperature of 30°C. After adding 5ml of sterile water to the solid medium, the spores of P. coronis Scrape off, place in a container filled with sterile wat...
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