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Liquid-base cell preservation liquid as well as preparation method and application thereof

A liquid-based cell and preservation solution technology, applied in the biological field, can solve the problems of unstable performance, low market share, poor preservation effect, etc., and achieve the effect of good detection of cells, prevention of cell aggregation, and convenient industrial production

Inactive Publication Date: 2018-11-09
生工生物工程(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current domestic research and development of cell preservation solutions often have problems such as unstable performance and poor preservation effect, and the market share is not high.

Method used

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  • Liquid-base cell preservation liquid as well as preparation method and application thereof
  • Liquid-base cell preservation liquid as well as preparation method and application thereof
  • Liquid-base cell preservation liquid as well as preparation method and application thereof

Examples

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preparation example Construction

[0058] According to a second aspect of the present invention, there is provided a method for preparing a liquid-based cell preservation solution, which is characterized in that it comprises the following steps:

[0059] The liquid-based cell preservation solution is obtained by uniformly mixing the fixative, anticoagulant, buffer, ionic strength maintaining agent, antimicrobial agent, mucolytic agent and cleaning agent in the formulated amount.

[0060] In the preparation method, the liquid-based cell preservation solution is obtained by uniformly mixing the components in the formula quantity, and the preparation method is simple in operation and convenient for industrialized production.

[0061] According to the third aspect of the present invention, the application of liquid-based cell preservation solution in the preservation of human exfoliated cells is provided;

[0062] In some embodiments of the present invention, human exfoliated cells include one or more of cervical e...

Embodiment 1

[0066] A liquid-based cell preservation solution, comprising the following components in parts by weight: 12 parts of ethanol, EDTA·2Na·2H 2 8 parts of O, 2 parts of sodium acetate buffer, 0.85 part of sodium chloride, 2 parts of ampicillin, 0.2 part of N-acetyl-L-cysteine ​​and 0.1 part of Tween-20; among them, the pH of sodium acetate buffer The value is 4.

Embodiment 2

[0068] A liquid-based cell preservation solution, comprising the following components in parts by weight: 18 parts of ethanol, EDTA·2Na·2H 2 O2 parts, 10 parts of sodium acetate buffer, 0.5 parts of sodium chloride, 8 parts of ampicillin, 0.05 parts of N-acetyl-L-cysteine ​​and 0.4 parts of Tween-20; among them, the pH of sodium acetate buffer The value is 5.

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Abstract

The invention discloses liquid-base cell preservation liquid as well as a preparation method and application thereof, relating to the technical field of biology. The liquid-base cell preservation liquid contains the following components in parts by weight: 12-18 parts of a fixing agent, 2-8 parts of an anti-coagulation agent, 2-10 parts of a buffer agent, 0.5-0.8 part of an ion strength maintaining agent, 2-8 parts of an antimicrobial reagent, 0.05-0.2 part of a mucus dissolving agent and 0.1-0.4 part of a cleanser. The liquid-base cell preservation liquid has the advantages that the cell stability is high, cells are well fixed, the cost is low, the preserved cell forms are relatively complete, and the like. Besides, the invention further provides the preparation method of the liquid-basecell preservation liquid and application of the liquid-base cell preservation liquid in human body cast-off cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a liquid-based cell preservation solution and its preparation method and application. Background technique [0002] The incidence of cervical cancer ranks first in female reproductive tract malignancies. There are nearly 500,000 new cases of cervical cancer every year in the world, 83% of which occur in developing countries, and 131,500 new cases occur in my country, accounting for 28.8%. About 290,000 people die of cervical cancer every year in the world, and about 30,000 people in my country. Therefore, the prevention and treatment of cervical cancer has become a global public health problem. [0003] Thin-Cytologic Test (TCT) uses a liquid-based thin-layer cytology detection system to detect cervical cells and perform cytological classification and diagnosis. It is currently an advanced cervical cancer cell test in the world. Compared with the traditional cervical smear and Pap...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0231
Inventor 李乐林付康
Owner 生工生物工程(上海)股份有限公司
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