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Preparation method for pasha worm extract with immunity regulating function

A technology for regulating immunity and crawling sandworms, applied in food extraction, function of food ingredients, food science, etc., to achieve the effect of cellular immune protection and enhancement of the body's immune function

Inactive Publication Date: 2018-11-06
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the eyes of the folks, sand worms are a delicacy in people's eyes. They mainly appear in restaurants. There are no processed sand worms on the market.
At present, there is no literature or patent report on the regulation of the immune function of the body by sand crawlers.

Method used

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  • Preparation method for pasha worm extract with immunity regulating function
  • Preparation method for pasha worm extract with immunity regulating function
  • Preparation method for pasha worm extract with immunity regulating function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of a sand crawling worm extract for regulating immune function, comprising the following steps:

[0022] 1) Preparation of sand crawling worm coarse powder: Wash the fresh and alive sand crawling worm with pure water, remove the head, tail and viscera, wash and drain, and freeze quickly at -80°C (freeze hard within 20 minutes) for later use;

[0023] 2) Before extracting the active ingredient, thaw it with warm water at 25°C at a constant temperature, crush it into small pieces of 5mm with a blender, and freeze-dry it in a freeze dryer at -80°C for 36 hours; beat the dried worm body into a large size with a pulverizer 40-mesh sieve, sealed and stored at -20°C for later use;

[0024] 3) Extraction of sandworm extract: Add distilled water at a ratio of 1:2 to g liquid and ml, soak in warm water at 25°C for half an hour, extract with an ultrasonic drug processor, filter, take the filtrate, and put the filtrate Dry in a freeze dryer at -80°C for 36 hou...

Embodiment 2

[0026] A method for regulating immune function of sand crawling worm defatted powder, comprising the following steps:

[0027] 1) Preparation of sand crawling worm coarse powder: Wash the fresh and alive sand crawling worm with pure water, remove the head, tail and viscera, wash and drain, and freeze quickly at -80°C (freeze hard within 20 minutes) for later use;

[0028] 2) Before extracting the active ingredient, thaw it with warm water at 25°C at a constant temperature, crush it into small pieces of 5mm with a blender, and freeze-dry it in a freeze dryer at -80°C for 36 hours; beat the dried worm body into a large size with a pulverizer 40-mesh sieve, sealed and stored at -20°C for later use;

[0029] 3), add petroleum ether to the spare substance finally obtained in step 2) according to the ratio of 1:5-1:10 of solid-liquid ratio, reflux in a water bath at 20-40°C for 2 hours, filter and recover petroleum ether, and obtain the defatted sand crawler pink.

Embodiment 3

[0031] A preparation process for regulating the immune function of sand crawling insect water extract coarse powder, comprising the following steps:

[0032] 1) Preparation of sand crawling worm coarse powder: Wash the fresh and alive sand crawling worm with pure water, remove the head, tail and viscera, wash and drain, and freeze quickly at -80°C (freeze hard within 20 minutes) for later use;

[0033] 2) Before extracting the active ingredient, thaw it with warm water at 25°C at a constant temperature, crush it into small pieces of 5mm with a blender, and freeze-dry it in a freeze dryer at -80°C for 36 hours; beat the dried worm body into a large size with a pulverizer 40-mesh sieve, sealed and stored at -20°C for later use;

[0034] 3), add petroleum ether to the spare substance finally obtained in step 2) according to the ratio of 1:5-1:10 of solid-liquid ratio, reflux in a water bath at 20-40°C for 2 hours, filter and recover petroleum ether, and obtain the defatted sand c...

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Abstract

The invention belongs to the field of functional food preparation, and specifically discloses a preparation method for a pasha worm extract with an immunity regulating function. The method comprises the following steps: step 1) cleaning fresh pasha worms with pure water, removing heads, tails and internal organs, cleaning and draining, and quickly freezing at a temperature of -80 DEG C for later use; 2) before extracting active ingredients, crushing into 5 mm small pieces after unfreezing at a constant temperature in warm water of 25 DEG C, freezing and drying for 36 hours in a freeze-drying machine, re-crushing until materials pass through a 40-mesh sieve, and sealing at the temperature of -20 DEG C for later use; 3) adding distilled water according to a solid-liquid ratio of 1 :2 to 1:4,soaking in warm water of 25 DEG C, using an ultrasonic drug processor to extract and filter, taking filtrate, and drying the filtrate on the freeze-drying machine to dry for 36 hours, and collectinga dried sample. The obtained pasha worm extract realizes immune protection on mice cells with low immunity; and pasha worm aqueous extract has obvious protection effect on special humoral immunity ofthe mice with low immunity.

Description

technical field [0001] The invention belongs to the field of functional food preparation, in particular to a preparation method of sand crawler extract for regulating immune function. Background technique [0002] Climbing sandworm is a famous and precious dietary tonic for ethnic minorities in my country. It is as famous as ginseng and antler, and it is also a good medicine respected by traditional ethnic minority medicine. The protein content in the dry matter of sand crawling worms is very high, 67.69%; the fat content is low, 10.4%; the total sugar content is very low, 1.59%; the ash content is 7.05%. Modern Chinese medical research believes that sandworms are sweet and warm in nature, enter the kidney and lung meridians, and have the effects of nourishing the lungs and kidneys, relieving coughs, benefiting deficiency, and strengthening essence. It is suitable for lung and kidney deficiency, insufficient essence, and impotence. Nocturnal emission, cough and shortness of...

Claims

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Application Information

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IPC IPC(8): A23L33/10
CPCA23V2002/00A23L33/10A23V2200/30A23V2200/324A23V2250/204A23V2300/14
Inventor 潘明张代芳陈欲云
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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