Method for detecting red tide and toxicity of seawater algae based on three-dimensional fluorescence spectrum

Abstract

The invention discloses a method for detecting red tide and toxicity of seawater algae based on a three-dimensional fluorescence spectrum. The method comprises the following steps: irradiating to-be-detected seawater by monochromatic ultraviolet light; collecting seawater reflected fluorescence; enabling the reflected fluorescence to generate the three-dimensional fluorescence spectrum via a diffraction grating; converting the three-dimensional fluorescence spectrum in an optical signal form into a three-dimensional fluorescence spectrum in an electric signal form by virtue of a CCD (Charge Coupled Device) so as to obtain original three-dimensional fluorescence spectrum data of the seawater; performing parallel representation on a quaternion matrix of the original three-dimensional fluorescence spectrum data; extracting spectral features based on quaternion principal component analysis; and establishing a nonlinear mapping model for determining hemolytic activity of red-tide algae, substituting the extracted spectral features into the established model, outputting the hemolytic activity value by the model, and determining the toxicity of the algae based on the value. The method disclosed by the invention is used for detecting based on a spectroscopic method, and no chemical reagent is added; the equipment used by the detection method is small and high in integrated level, and continuous in-situ online measurement can be realized; and the detection accuracy is high.

Description

technical field [0001] The invention belongs to the technical field of photoelectric detection, and relates to a method for detecting seawater algae red tide and toxicity based on three-dimensional fluorescence spectrum. Background technique [0002] Harmful algal bloom (HAB), also known as red tide, is a harmful ecological phenomenon in which some phytoplankton, protozoa or bacteria explosively multiply or highly gather in seawater under certain environmental conditions, causing water discoloration. Red tides can sometimes cause great harm to fish and other aquatic animals. This is caused by red tide plankton clogging fish gills, causing mechanical obstacles, and decomposing after death, rapidly consuming oxygen, insufficient oxygen in the water, and secreting harmful substances. Ichthyotoxic red tide can produce a variety of toxic substances, causing a large number of fish and shellfish to die in a short period of time. Fish-toxic red tide algae have been widely distribut...

AI Technical Summary

Problems solved by technology

Because the absorption spectrum of phytoplankton is easily affected by yellow substances and photoprotective pigments, the further development of pigment spectroscopy is limited.

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