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Method for preparing senecio scandens root tip chromosome slide

A production method and a technology of thousand miles of light, applied in the field of chromosome production, can solve problems such as unspecified varieties, and achieve the effects of facilitating cell dispersion, preventing tissue overlap, and increasing the number of

Inactive Publication Date: 2018-11-02
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, some studies have found that the pyrrolizidine alkaloids in the plant components of Senecio have certain hepatotoxicity, mutagenic toxicity, embryotoxicity, etc., but did not point out the specific species. How to identify whether the poisoned species is similar to certain species in my country It is very important for the development and utilization of Chinese herbal medicines of the genus Senecio to be of the same species

Method used

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  • Method for preparing senecio scandens root tip chromosome slide
  • Method for preparing senecio scandens root tip chromosome slide
  • Method for preparing senecio scandens root tip chromosome slide

Examples

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Embodiment 1

[0025] A method for making a Senecio root tip chromosome specimen sheet: the material comes from the F1 generation obtained by crossing Zunyi wild high antibacterial Senecio and low antibacterial Senecio, and hydroponics For the young shoots of the F1 generation, when the root length is about 2-3cm, cut off the 0.5cm-long root tip, then put the root tip into the ice-water mixture for 17 hours, take it out after soaking, and use 0.075mol / L KCL solution for hypotonicity Treat for 30min. The root tips after hypotonic treatment were washed with double distilled water, and then fixed with Carnoy's fixative solution (absolute ethanol: glacial acetic acid = 3:1, V / V) for 36 h. The fixed root tips were taken out, washed with water for 30 minutes, and then enzymatically hydrolyzed with a mixed enzyme solution of 2.5% cellulase and 2.5% pectinase at 37°C for 90 minutes; after enzymatic hydrolysis, the root tips were hypotonic in double distilled water for 40 minutes. Production: Cut ou...

Embodiment 2

[0028]A method for making a chromosomal specimen sheet of Senecio root tip: hydroponic young young branches of wild Senecio in Zunyi, Guizhou, and when the root length is about 2 to 3 cm, cut the root tip with a length of 0.5 cm, and then put the root tip in ice Soak in the water mixture for 16 hours, take it out after soaking, and treat it with 0.075mol / L KCL solution for 30 minutes. The root tips after hypotonic treatment were washed with double distilled water, and then fixed with Carnoy's fixative solution (absolute ethanol: glacial acetic acid = 3:1, V / V) for 48 hours. The fixed root tip was taken out, washed with water for 30 minutes, and then hydrolyzed with a mixed enzyme solution of 2.5% cellulase and 2.5% pectinase at 37°C for 70 minutes, and then hypotonic in double distilled water for 40 minutes. Production: Cut out the root tip meristematic zone, place it on an ice slide, add a small amount of double distilled water dropwise to keep the root tip in a liquid enviro...

Embodiment 3

[0031] A method for making a Senecio root tip chromosome specimen sheet: the material comes from the F1 generation obtained by crossing Zunyi wild high antibacterial Senecio and low antibacterial Senecio, and hydroponics For the young shoots of the F1 generation, when the root length is about 2-3cm, cut off the 0.5cm-long root tip, then put the root tip into the ice-water mixture for 17 hours, take it out after soaking, and use 0.075mol / L KCL solution for hypotonicity Treat for 30min. The root tips after hypotonic treatment were washed with double distilled water, and then fixed with Carnoy's fixative solution (absolute ethanol: glacial acetic acid = 3:1, V / V) for 58 hours. Take out the fixed root tip, wash it with double distilled water for 3-5 times, and use a mixed enzyme solution of 2.5% cellulase and 2.5% pectinase to enzymolyze it at 37°C for 90 minutes; 40min. Production: cut the root tip meristematic zone, place it on an ice slide, add a small amount of double-distil...

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Abstract

The invention discloses a method for preparing a senecio scandens root tip chromosome slide in the technical field of chromosome slide preparation. The method comprises the following steps: firstly, selecting an intact root tip on a senecio scandens plant; secondly, placing the root tip in an ice water mixture for soaking pretreatment for 15-17 h; thirdly, performing hypotonic treatment on the root tip with a KCl solution; fourthly, cleaning the root tip after hypotonic treatment with double distilled water, and then performing fixing with Carnoy's fixative; fifthly, washing the fixed root tips with double distilled water, and then performing enzymolysis with a mixed enzyme solution; sixthly, washing the root tip after enzymolysis with double distilled water and performing hypotonic treatment with double distilled water for 40-60 min; seventhly, cutting the front end of the root tip by 2-3 mm and placing the front end of the root tip on an ice slide, then scattering root tip tissue, adding dropwise Carnoy's fixative and a modified phenol fuchsine solution, covering a cover slip after dyeing for 15-20 min, and then knocking, baking and pressing the cover slip to obtain the senecio scandens root tip chromosome slide. The senecio scandens root tip chromosome slide prepared with the scheme has the characteristics of good dispersion, clear shape and high recognition.

Description

technical field [0001] The invention belongs to the technical field of chromosome preparation, and in particular relates to a method for making a Senecio root tip chromosome specimen sheet. Background technique [0002] Senecio is a plant of the genus Senecio in the family Asteraceae, also known as Jiuliming. It is widely distributed in my country, with rich wild resources and complex chemical components. Light water extract has certain inhibitory effect on HIV-1 virus), anti-leptohelical, anti-trichomonas and other pharmacological effects. It is mainly used to treat influenza, pneumonia, mumps, acute enteritis, acute urinary tract infection, erysipelas, eczema, dry and wet ringworm sores, trichomonal vaginitis, burns and scalds, etc. With the continuous development of science and technology, the research on its chemical composition, pharmacological effects and toxicology has been continuously improved, so that Senecio has broad prospects for development and utilization. ...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/2813
Inventor 李林杨春先余金芮黄金金钱刚徐德林谢欣上官燕妮
Owner ZUNYI MEDICAL UNIVERSITY
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